最新一代视频压缩标准VP9综述

VP9是一个由谷歌开发的开放格式、无使用授权费的视频压缩标准,并做为谷歌推行WebM工程的一部分.对VP9标准的主要技术特征进行了分析,包括帧内预测、帧间预测、变换和量化、熵编码、环路滤波等,同时与其他先进的视频压缩标准HEVC进行了比较.     

埃可病毒9型分离株MSH-KM812 VP1基因的遗传特征

目的分析2010年昆明市引起手足口病(Hand,foot and mouth disease,HFMD)的病原埃可病毒9型(Echovirus 9,E9)分离株MSH-KM812 VP1基因的遗传特征。方法分别采用RD细胞和Hep-2细胞对3份HFMD患儿粪便标本进行病毒分离,应用RT-PCR法分2段扩增病毒VP1基因,并进行序列测定。应用NCBI BLAST软件对所测定的节段序列进行比对;Omiga软件对所测定的节段序列的核苷酸及推导的氨基酸序列进行编辑、比对和拼接;应用Mega 5.05软件进行序列分析,并与15个E9参考株的VP1基因序列进行比较,构建基因系统进化树。结果从3份HFMD患儿粪便标本中分离到1株E9,命名为MSH-KM812,其VP1区的核苷酸长度为888 bp。MSH-KM812株与其他15个E9参考株的核苷酸和氨基酸序列同源性分别为78.6%～98.0%和87.5%～99.3%,与中国分离株的核苷酸和氨基酸序列同源性分别为92.7%～98.0%和97.0%～99.3%,其中与中国江苏分离株M10MF37 China2010核苷酸和氨基酸序列同源性最高,分别为98.0%和99.3%,而与德国分离株Hill的核苷酸和氨基酸序列同源性最低,分别为78.6%和87.5%。基因进化树分析显示,MSH-KM812分离株与中国江苏分离株M10MF37 China 2010和中国山东分离株05189-SD-CHN-2005-E9属于同一个进化分枝。结论 MSH-KM812分离株为E9,与中国其他分离株同属一个进化分枝。     

VP技术在煤矿综采工作面设备配套中的应用

分析了目前煤矿综采工作面设备的使用情况,总结了综采工作面设备配套中存在的问题。在介绍VP技术的基础上,阐述了煤矿综采工作面设备配套VP系统的系统结构、各子系统的功能以及系统的实现方式。     

Palladium Nanoparticles on Thermoresponsive Hydrogels and their Application as Recyclable Suzuki–Miyaura Coupling Reaction Catalysts in Water

Palladium nanoparticles were immobilized on PNIPAM:4‐VP hydrogels. The resultant püalladium catalysts showed high activities for Suzuki–Miyaura coupling reactions in water. The recyclability of the catalysts was improved by using 4‐VP as a co‐monomer in the hydrogels due to reduced Pd leaching during the reactions.     

Ceramic and polymeric solid electrolytes for lithium-ion batteries

Lithium-ion batteries are important for energy storage in a wide variety of applications including consumer electronics, transportation and large-scale energy production. The performance of lithium-ion batteries depends on the materials used. One critical component is the electrolyte, which is the focus of this paper. In particular, inorganic ceramic and organic polymer solid-electrolyte materials are reviewed. Solid electrolytes provide advantages in terms of simplicity of design and operational safety, but typically have conductivities that are lower than those of organic liquid electrolytes. This paper provides a comparison of the conductivities of solid-electrolyte materials being used or developed for use in lithium-ion batteries.     

ATM自愈网中VP交换机对备用VP自愈算法性能的影响分析

在ATM自愈网采用备用VP自愈算法的条件下,对VP交换机中几种典型的自愈功能实现方案建立了相应的排队模型并进行了大量的计算机模拟试验,详尽分析了VP交换机中自愈功能实现方案以及一些重要结构参数对自愈性能的影响。所用的分析方法具有普遍意义,得到的分析结果对于如何确定VP交换机中自愈功能实现方案、重要结构参数、备用VP的数目,以实现最好的自愈性能,具有指导意义。     

虚拟样机技术在变速箱设计中的研究与应用

以圆柱齿轮变速箱为例对虚拟样机技术、仿真技术进行了深入的分析研究,提出了实施虚拟样机的技术路线和具体的实现步骤,研究了三维建模软件Pro/E与动力学仿真分析软件AD-AMS之间的接口技术,成功实现二者的联合仿真,为以后的产品设计开发提供了好的借鉴方法。     

基于XML的智能车载导航系统和富信息交互平台

近年来,广大普通汽车用户越来越渴求大量有效的数据服务、通信服务、强大的娱乐支持和高保障的安全防盗系统.本设计充分利用了Intel Gene8310板的硬件资源,扩展了大量外围硬件,并配以拥有强大图形处理能力的Windows系统,呈现了十分良好的用户接口界面.系统采用了丰富的应用技术实现了双屏幕独立显示、双触摸屏、个性化的人机交互方式、手势及语音命令识别、GPS汽车导航、卫星地图导航、富信息、多媒体等等功能,极大满足了普通汽车用户的增值享受需求,给出了富信息、多功能型车载系统的完整的解决方案,真正构建了一个功能强大、可扩展性强的智能车载导航系统和富信息平台.     

Quantitative Proteomic Analysis of Escherichia coli Heat-Labile Toxin B Subunit (LTB) with Enterovirus 71 (EV71) Subunit VP1

The nontoxic heat-labile toxin (LT) B subunit (LTB) was used as mucosal adjuvant experimentally. However, the mechanism of LTB adjuvant was still unclear. The LTB and enterovirus 71 (EV71) VP1 subunit (EVP1) were constructed in pET32 and expressed in E. coli BL21, respectively. The immunogenicity of purified EVP1 and the adjuvanticity of LTB were evaluated via intranasal immunization EVP1 plus LTB in Balb/c mice. In order to elucidate the proteome change triggered by the adjuvant of LTB, the proteomic profiles of LTB, EVP1, and LTB plus EVP1 were quantitatively analyzed by iTRAQ-LC-MS/MS (isobaric tags for relative and absolute quantitation; liquid chromatography-tandem mass spectrometry) in murine macrophage RAW264.7. The proteomic data were analyzed by bioinformatics and validated by western blot analysis. The predicted protein interactions were confirmed using LTB pull-down and the LTB processing pathway was validated by confocal microscopy. The results showed that LTB significantly boosted EVP1 specific systematic and mucosal antibodies. A total of 3666 differential proteins were identified in the three groups. Pathway enrichment of proteomic data predicted that LTB upregulated the specific and dominant MAPK (mitogen-activated protein kinase) signaling pathway and the protein processing in endoplasmic reticulum (PPER) pathway, whereas LTB or EVP1 did not significantly upregulate these two signaling pathways. Confocal microscopy and LTB pull-down assays confirmed that the LTB adjuvant was endocytosed and processed through endocytosis (ENS)-lysosomal-endoplasmic reticulum (ER) system.