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June R. Lawton 《Journal of microscopy》1990,158(3):343-354
Malachite green and neutral red, when added to glutaraldehyde for fixation of various tissues, yielded high-contrast images of cell ultrastructure. Malachite green, in acid conditions, appeared to increase contrast of heterochromatin material in the nucleus whereas neutral red gave greater clarity to the nucleolus and to cytoplasmic ribosomes. Control tissue fixed under acid conditions showed little damage but there were ‘crystalline’ areas at the periphery of the nucleolus. RNase did not digest cytoplasmic ribosomes from tissue after neutral red glutaraldehyde fixation. These results suggested that neutral red became bound to RNA in the tissues. Fixation with malachite green, at a pH below 6, did not affect the digestion of RNA by RNase but did protect chromatin against the bleaching action of the chelating agent EDTA. The addition of malachite green (pH < 6) or neutral red to glutaraldehyde are useful techniques for the investigation of the ultrastructure of nuclear material and cytoplasmic ribosomes. 相似文献
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庞朝霞 《信息工程大学学报》1994,(1)
本文论述了英语科技术语的结构、形式、词义之间的联系,其目的是揭示英语词汇的某些基本特点,帮助读者分析、理解并恰当使用科技术语。 相似文献
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Abstract. A simplified version of the square root Kalman filter is obtained for a vector autoregressive moving-average (VARMA) model. The algorithm is computationally more efficient that the standard square root algorithm and its output can be used to compute the likelihood of a VARMA model accurately. 相似文献
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Tarun Ch. Sarmah Mahinath Dev Choudhury Monoranjan Goswami 《Journal of the science of food and agriculture》1987,41(2):107-113
Assay conditions in vivo for determination of nitrate reductase activity (NRA) in leaf and feeder root tissues of tea bushes (Camellia sinensis L.) were studied. The composition of the incubation mixture, pH, temperature, period of incubation and tea shoot components were varied. Maximum NRA was obtained in leaf with a medium containing 300 mg Polyclar AT with 300 mg leaf disc in 0.1 M phosphate buffer at pH 7-5, 0.02 M KNO3 in a total volume of 5 ml and incubated for 4 hat 30°C in the dark. Propan-1-ol inhibited NRA in tea leaf and root. Highest activity was found in the first leaf. 相似文献
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David B. Dusenbery 《Journal of chemical ecology》1987,13(7):1617-1624
A theory used to estimate the smallest relative chemical gradient that is potentially detectable is given approximately byG>(DCR
2t3)–1/2 whereD is the diffusion constant andC the concentration of the stimulus chemical,R is the effective radius of the receptor, the velocity of the organism, andt the time period within which the organism measures the concentration. Of these factors, time has the strongest influence. Combining this result with available information on gradients of carbon dioxide around roots and behavior of bacteria and nematodes leads to several conclusions. Bacteria can potentially detect carbon dioxide gradients in the layer of water in contact with the root but not further away. In contrast, plant-parasitic nematodes can potentially detect gradients one meter from a single long root fiber and over 2 m from a plant root mass using klinokinesis. A direct approach using klinotaxis can start a few centimeters from a single root fiber and half a meter from a root mass. These differences are due to differences in the time available to measure the concentrations. Increasing the length of a bacterium could increase its ability to detect gradients by reducing its rotational diffusion. Collimating stimuli that serve to maintain a straight path may provide a means of improving chemotaxis by permitting concentration to be measured for longer times during klinokinesis. More accurate predictions can be made when more precise data are available. The analysis is applicable to a wide variety of other organisms and stimuli. 相似文献
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次氯酸根(ClO-)作为消毒剂和漂白剂的有效成分,常用于的人类生产和生活中。在狭小密闭空间内使用含有次氯酸根的消毒剂存在一定安全隐患,同时ClO-在体内的参与人体的多项生命活动,过量的ClO-存在于人体会对人体的免疫系统造成极大危害,诱发多种疾病。因此检测ClO-是十分必要的。本文采用一锅法,以聚乙烯亚胺为配体合成一种硫化镉量子点荧光探针实现对ClO- 的检测,结果显示,探针对ClO- 的识别具有较高的选择性和抗干扰能力,其检测范围在0~40 nmol/L,检测限为0.5 nmol/L。 相似文献