Analysis of the Structure and Biosynthesis of the Lipopolysaccharide Core Oligosaccharide of Pseudomonas syringae pv. tomato DC3000

Lipopolysaccharide (LPS), the major component of the outer membrane of Gram-negative bacteria, is important for bacterial viability in general and host–pathogen interactions in particular. Negative charges at its core oligosaccharide (core-OS) contribute to membrane integrity through bridging interactions with divalent cations. The molecular structure and synthesis of the core-OS have been resolved in various bacteria including the mammalian pathogen Pseudomonas aeruginosa. A few core-OS structures of plant-associated Pseudomonas strains have been solved to date, but the genetic components of the underlying biosynthesis remained unclear. We conducted a comparative genome analysis of the core-OS gene cluster in Pseudomonas syringae pv. tomato (Pst) DC3000, a widely used model pathogen in plant–microbe interactions, within the P. syringae species complex and to other plant-associated Pseudomonas strains. Our results suggest a genetic and structural conservation of the inner core-OS but variation in outer core-OS composition within the P. syringae species complex. Structural analysis of the core-OS of Pst DC3000 shows an uncommonly high phosphorylation and presence of an O-acetylated sugar. Finally, we combined the results of our genomic survey with available structure information to estimate the core-OS composition of other Pseudomonas species.     

阶梯形零件的冷挤压工艺研究

详细介绍了阶梯形零件冷挤压工艺的制订过程，根据实践经验总结了其冷挤压模具设计的结构特点。     

3Cr2MnNiMo钢大型模块的预硬化处理

本文试验研究了八种淬火冷却方式和四种回火工艺对3Cr2MnNiMo钢预硬化处理后的硬度的影响，在所得的试验数据的基础上，分析估计了3Cr2MnNiMo钢大模块硬化后的表心硬度差，该差值与工业生产结果大致相吻合。     

一种基于分布约简的规则获取方法*

基于决策表分布约简定义规则的分布核与分布约简概念,提出一种基于分布约简的规则获取方法.首先确定条件类的分布核,进而采用启发式算法计算其分布约简,挖掘出最简产生式规则.该方法能适应决策表的不一致情形,且运用此方法所提取的规则集能够保持与原信息系统一致.     

加芯搅拌桩芯桩定位限垂技术的研发与应用

为控制水泥土加芯搅拌桩的芯桩贯入质量,研发了一种基于芯桩定位导向架的定位限垂挑臂装置,并通过采用不同材质、不同尺寸的方法,反复摸索、实施,分析得出了芯桩定位导向架的定位限垂挑臂装置的最优方案。同时,通过运用数据比对、图表说明的方法,有效验证了加芯搅拌桩芯桩定位限垂挑臂装置对于现场施工的重要作用。在新疆地区23450 根加芯搅拌桩工程中进行首次应用,实践表明研发的定位限垂技术可有效控制芯桩贯入的定位和垂直度,提高施工效率。该定位限垂技术经济效益良好,可为今后新疆地区特殊软基处理以及加芯搅拌桩施工提供参考价值。     

方波激励下纳米晶体铁心损耗模型建立与验证

为了实现电力机车小型化轻量化的发展要求,国内外专家考虑通过采用提高变压器的工作频率减小变压器整体的体积和质量。铁心作为高频变压器的关键部件,可以准确计算铁心损耗,对中高频变压器的设计和优化具有重要作用。针对高频变压器铁心通常是工作在方波或脉宽调制(PWM)波等非正弦激励下的特点,对传统Steinmetz损耗计算模型进行了优化改进,给出了考虑磁密的变化率及波形系数对损耗的影响的Steinmetz改进损耗模型。同时,为了提高损耗模型在不同特征频率下的通用性,进一步对损耗模型系数的非线性进行研究,给出了模型系数随频率变化的非线性函数。最后,利用有限元计算结果同实物测量结果进行比较,证明了所提损耗模型在方波激励下对纳米晶体铁心计算的准确性。     

高压输电线路在线监测设备无线供电磁耦合机构优化

对高压输电线路进行实时精准的在线监测是保障电网稳定运行的有效手段。磁耦合共振无线供电为解决在线监测设备供电困难的问题提供了一种可行方案,但传统磁耦合共振系统耦合程度弱,传输效率对传输距离变化敏感,总体性能欠佳。基于电路模型分析了耦合线圈互感对传输效率的影响,提出了考虑线圈与磁芯耦合影响关系的联合优化方案从而提升传输性能。仿真和实验结果验证了在同轴间距、水平和角度偏移变化时的系统效率提升。为提升磁耦合系统传输效率和距离提供了一种有效方案。     

Effects of micro-sized mullite on cristobalite crystallization and properties of silica-based ceramic cores

Silica-based ceramic cores are extensively used in investment casting process, during which they must exhibit sufficient flexural strength and deformation resistance. In this study, micro-sized mullite was used as an additive to silica-based ceramic cores to optimize their high temperature properties. To investigate the effects of micro-sized mullite on cristobalite crystallization, mechanical and thermal properties of silica-based ceramic cores, ceramic cores with different amounts of micro-sized mullite were fabricated. The XRD results showed that additional micro-sized mullite diminished the crystallization of cristobalite at high temperatures, primarily caused by the mullite related compressive stresses on the surface regions of fused silica particles. Three-point bending tests and SEM results showed that micro-sized mullite had a more significant effect on the flexural strength of ceramic cores compared with conventional additives. Particularly, the fracture mechanism of silica-based ceramic cores had been changed from intergranular fracture into a mixed fracture consisting of both intergranular and transgranular fracture. The mechanical and thermal properties of ceramic cores were all reduced slightly as the mullite content exceed 4.6 wt%. Hence, to optimize the properties of silica-based ceramic cores, the micro-sized mullite content should not exceed 4.6 wt%.     

铜基薄壁热管应用现状及发展趋势

在5G通信技术及消费电子行业快速发展的背景下,铜基薄壁热管被广泛应用于大功率电子设备散热领域。通过介绍目前应用较广的几种热管,对其结构、特点及应用范围进行了对比 ;阐述热管在加工制备和使用过程中存在的一些问题和缺陷,对表面缺陷形成机理进行分析,并对热管未来的发展趋势进行了展望。     

变压器叠铁心智能制造技术研究

重点论述了智能制造的重要性及叠铁心材料和智能制造技术中的智能物料、智能柔性产线、智能装备、智能数字化设备模型、智能数字化质量模型、智能物流、智能服务、极致呵护等方面内容,对铁心产业发展有一定的借鉴价值。