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排序方式: 共有143条查询结果,搜索用时 15 毫秒
11.
Barbara M. Rothen-Rutishauser Marius J. Messerli Hans van der Voort Maja Günthert Heidi Wunderli-Allenspach 《Journal of Computer-Assisted Microscopy》1998,10(3):103-111
The relative spatial distribution of proteins was investigated with immunofluorescent methods by confocal laser scanning microscopy and digital image restoration. For confocal data sets recorded with a voxel dimension of 50 × 50 × 150 nm noise and blur can be decreased and the resolution in the z-axis increased by applying the maximum likelihood estimation algorithm of the Huygens software. This approach was successfully applied to the study of tight and adherens junctions in relation to the actin cytoskeleton in Madin Darby Canine Kidney cells. Colocalization analysis was done for pairs of probes using a histogram-based method. F-actin, occludin, zonula occludens 1, and E-cadherin were included in the study. Double-labeled preparations were used. The combination of deconvolution with the colocalization of confocal data sets offers a powerful tool to investigate the spatial arrangement of proteins. 相似文献
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《International Journal of Hydrogen Energy》2023,48(44):16601-16613
The multistep kinetics of Fe2O3 reduction by H2 is investigated by data deconvolution in this study. The reduction process was conducted in a TGA apparatus isothermally in the temperature range of 750–950 °C. The stepwise reduction of Fe2O3, i.e. Fe2O3–Fe3O4, Fe3O4–FeO, and FeO–Fe, was successfully decoupled from each other without controlling the reduction gas atmosphere. The overlapping, as well as the reaction rate, of each reduction step can be described by the deconvoluted data with R2 > 0.995 for all the tested temperatures. Based on the deconvolution, relatively stable activation energy with increasing the conversion was obtained for each step with the model-free iso-conversion method, indicating the rationality of the decoupled multistep profiles. Master plot was then applied to evaluate the suitability of kinetic models reported in the open literature. The JMA model (Avrami-Erofe'ev equation), corresponding to the nucleation and growth mechanism, was found to be most suitable for describing each reduction step. The activation energies obtained by the JMA model fitting for Fe2O3–Fe3O4, Fe3O4–FeO, and FeO–Fe were 10.3, 26.7, and 24.8 kJ/mol, respectively, which also agree well with the Ea obtained by the model-free method. 相似文献
14.
V. Székely 《Microelectronics Journal》2010,41(9):560-565
Thermal transient recording and the time constant spectrum analysis are widely used methods in the thermal testing and qualification of IC packages. A limitation of these methods is that recording of the complete transient response requires long time. This limitation, however, can be overcome by sophisticated procedures. The first method is to apply short power pulse for excitation; the second one is the interruption of the transients long before the thermal equilibrium is reached. The paper offers algorithms to evaluate these short pulse and short time measurements. The presented methods are suitable if the extraction of the little time constants is needed. This is the case if the transient method is used e.g. for die attach quality checking. 相似文献
15.
Catalina E. Alupoaei 《Chemical Engineering Communications》2013,200(2):198-218
Multiwavelength UV-vis spectra of microorganisms and cell suspensions contain quantitative information on their properties such as number, size, shape, chemical composition, and internal structure. These properties are essential for the identification and classification of cells. The complexity of microorganisms in terms of their chemical composition and internal structure makes the interpretation of their spectral signature a difficult task. In this article a model is proposed for the interpretation of the multiwavelength spectra of microorganisms. The proposed interpretation model is based on light scattering theory, spectral deconvolution techniques, and the approximation of the frequency-dependent optical properties of the basic constituents of living organisms. The optical properties as functions of wavelength and available literature data on the size and chemical composition of E. coli cells and Bacillus globigii spores were used to explore the sensitivity of the calculated spectra to the model parameters. It is shown that the proposed model can reproduce the features of experimentally measured spectra. The sensitivity of the spectra to the model parameters suggests that the proposed model can be used for the quantitative deconvolution of the UV-vis spectra in terms of critical information necessary for the detection and identification of microorganisms. 相似文献
16.
Wang Yanfei 《电子科学学刊(英文版)》1997,14(2):112-116
A new deconvolution method has been presented in this paper. Like conventional Discrete Fourier Transform(DFT) method, it is also a frequency domain method. Since there are only DFT, IDFT and multiplication calculations in the new method, the singular problem appeared in the conventional DFT method can be avoid, and the deconvolution problem can always be solved with the new method. The relationship between the presented method and conventional DFT method is discussed. A numerical example is also presented to illustrate the new method. 相似文献
17.
本文研究了混合相位子波的反滤波因子、稳定性及最小绝对误差反滤波因子的渐近性质.叙述了输入信号与L_1模反褶积解的稳定性的关系.文中给出了白噪化的处理方法,并用实例说明了白噪化的效果. 相似文献
18.
Kernel deconvolution of stochastic volatility models 总被引:2,自引:0,他引:2
Fabienne Comte 《时间序列分析杂志》2004,25(4):563-582
Abstract. In this paper, we study the problem of the nonparametric estimation of the function m in a stochastic volatility model h t = exp( X t /2λ) ξ t , X t = m ( X t −1 ) + η t , where ξ t is a Gaussian white noise. We show that the model can be written as an autoregression with errors-in-variables. Then an adaptation of the deconvolution kernel estimator proposed by Fan and Truong [ Annals of Statistics , 21, (1993) 1900] estimates the function m with the optimal rate, which depends on the distribution of the measurement error. The rates vary from powers of n to powers of ln( n ) depending on the rate of decay near infinity of the characteristic function of this noise. The performance of the method are studied by some simulation experiments and some real data are also examined. 相似文献
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20.
Roman Hovorka Paul A. Soons Malcolm A. Young 《Computer methods and programs in biomedicine》1996,50(3):253-264
ISEC (Insulin SECretion) is a computer program which calculates pre-hepatic insulin secretion from plasma C-peptide measurements. The program uses a regression (population) model to derive parameters of C-peptide kinetics from subject's gender, type (normal, obese, non-insulin dependent diabetes mellitus), age, weight, and height. Insulin secretion is calculated as a piece-wise constant (step) function with flexible step length allowing for a fine resolution of the secretion profile between measurements. A constrained regularisation method of deconvolution is employed to carry out the calculations. The calculated profile satisfies three properties: (i) it fits the measurement within the given level of the measurement error, (ii) it is non-negative, and (iii) it has a minimum value of a regularisation criterion (norm of second differences) which quantifies the degree of deviation of the secretion profile from a straight line. Both theoretical aspects and specific features related to ISEC are considered. To exemplify the use of ISEC, pre-hepatic insulin secretion is calculated during meal tolerance test, frequently sampled intravenous glucose tolerance test, hyperinsulinaemic euglycaemic glucose clamp, and basal conditions with frequent sampling. 相似文献