腺嘌呤磷酸盐锁核酸的合成

以双丙酮-D-葡萄糖为起始原料,通过氧化,还原,亲核取代,水解等19步反应合成标题化合物,总产率8.5%,其结构通过1HNMR和MS表征。     

一种苯磺酸酯类光致产酸剂的合成与性能研究

以苯酐、盐酸羟胺和4-甲基苯磺酰氯为原料,合成了N-羟基邻苯二甲酰亚胺对甲苯磺酸酯,并利用红外、核磁共振和紫外对其进行表征,研究了溶解性、紫外吸收和光刻等性能。结果表明,该产酸剂在常见有机溶剂中表现出良好的溶解性,并在248 nm处有好的透明性,可应用于深紫外光致抗蚀剂体系。     

STUDY ON BIOLEACHING OF PRIMARY CHALCOPYRITE ORE WITH THERMOACIDOPHILIC ARCHAE

1. Introduction There have been two difficulties encountered with the leaching of low-grade primary chalcopyrite oresusingmesophile culture-thiobacillusferrooxidans(Tf) and thiobacillusthiooxidans(Tt), and so on[1-3].The first is the poor leaching effect and the second is the resultant increase in temperature during the middle and the later phase ofheap-leachingperiod, which is unfavorable for the growth and the culture of Tf and Tt. Being widely used for mineral leaching at present, Tf and …     

Impact toughness and fracture toughness of austempered ductile iron

1.IntFOdUction AustemPered Duetile Iron(ADI)15 a relatively newmaterial in east alloy family.Its mierostrueture eonsists ofaeieular ferrite in a high earbon austenite matrix ealledausferrite.This very fine mierostrueture offers     

Effects of Cerium on Rooting and Physiological-Biochemical Metabolism of Tissue Culture Plantlet from Dioscorea Zingberensis

Effects of Cerium on Rooting and Physiological-Biochemical Metabolism of Tissue Culture Plantlet from Dioscorea Zingberensis     

Polyol-Mediated Synthesis and Photoluminescent Properties of LaPO_4∶Eu ~( 3+) Nanoparticles

Polyol-Mediated Synthesis and Photoluminescent Properties of LaPO_4∶Eu ~( 3+) Nanoparticles     

0.18μm CMOS, Monolithic MSTP ASIC

I. Introduction Motivated by adopting both telecom and data-com traffic into Synchronous Digital Hierarchy (SDH)[1] transport payloads, we develop the mono-lithic Multi-Service Transport Platform (MSTP)[2] Application Specified Integrated Circuit (ASIC) MSEOSX8-6, which is a highly integrated device capable of mapping 10/100/1000Mbit/s Ethernet[3], 155Mbit/s Resilient Packet Ring (RPR)[4], as well as 2.048Mbit/s E1 traffic into SDH STM-1 payloads. On the line side, the chip s…     

Microstructure refinement of Sn-Sb Peritectic alloy by power ultrasonic

Sn-Sb peritectic alloy was treated with 400 W, 600 W and 800 W ultrasonic, respectively, to study the effect of power ultrasonic on solidification microstructure. Results show that power ultrasonic can refine α phase and β phase of the alloy, as well as eliminate gravitational segregation. Moreover, 600 W ultrasonic demonstrated the best refining effect, not only homogenous and fine solidification microstructure can be obtained, the cubical β phase crystals can also be spheroidized.     

基于LESO的海上风机塔架振动无源控制

以海上永磁同步风力发电系统为研究对象,考虑风、波浪载荷对海上风机支撑结构的影响,研究塔架前后/两侧振动的控制及干扰抑制问题。将波浪载荷作为外部干扰,提出基于线性扩张状态观测器的塔架振动无源控制方法。首先,根据海上塔架振动的基本模型建立反映系统物理结构特性的端口受控哈密尔顿(port-controlledHamiltonian,PCH)模型。然后,进行无源控制器(passivity-based controller,PBC)和线性扩张状态观测器(linear extended state observer,LESO)的设计,通过LESO对干扰进行观测,无源控制器用于实现塔架振动的抑制,并对LESO的估计能力和闭环系统的稳定性进行理论分析。最后,与线性自抗扰控制(linearactive disturbancerejectioncontrol,LADRC)策略和无源控制策略进行仿真对比,仿真结果表明提出控制策略的有效性。     

Preparation of liposome-coated oligonucleotide labeled with ^99mTC and its uptake in vascular smooth muscle cells

To explore the preparation method of liposome-coated 99mTc-labeled antisense oligonucleotide (ASON),targeteing the proliferating cell nuclear antigen (PCNA), and to explore the biological characteristics and the uptake kinetics of a radiolabeled probe in vascular smooth muscle cells, an 18-base single-stranded antisense oligonucleotide targeting PCNA mRNA and the complementary strand (sense oligonucleotide, SON) were synthesized. The ASON (SON) was labeled with 99mTc, by conjugating the bifunctional chelator (hydrazino nicotinamide, HYNIC), and purified through a gel filtration column of Sephadex G-25. The product was then encapsulated in cationic liposome (oligofectamineTM). The radiolabeling efficiency, radiochemical purity, stability of the liposome-coated 99mTc-HYNIC-ASON in a phosphate buffered solution (PBS), and fresh human serum and its uptake rate were studied. There was no significant difference between the 99mTc radiolabeling efficiencies of HYNIC-ASON and HYNIC-SON, which were 60.04% ± 1.92% and 59.60% ± 2.53%, respectively (P ＞ 0.05, n = 5). The radiochemical purity of the liposome-coated 99mTc-HYNIC-ASON was 94.70% ± 1.90% (n = 5). And after incubation with PBS and fresh human seAt 90 min after transfection, the uptake rate of the liposome-coated 99mTc-HYNIC-ASON reached its peak of 83.8% ±5.92% in vascular smooth muscle cells (VSMCs) and was much higher than that of the nonliposome-coated 99mTc-HYNIC-ASON, which was 11.16% ± 0.54% (P ＜ 0.01, n = 4). The labeling method of PCNA ASON (SON) conjugated by HYNIC has been proved successful. The liposome was able to enhance the ASON (SON) uptake in VSMCs,and could be widely used as a safe, convenient, effective gene transfer carrier.