富铁面包酵母的筛选及富集条件研究

本文探索获得富铁面包酵母的富集条件。筛选获得一株富铁能力较强的面包酵母菌株QN006。通过单因素实验确定该菌株富铁工艺条件为:铁盐种类为硫酸亚铁,亚铁离子浓度为900μg/mL,添加时间为0h,温度为28℃,pH为5.0。在此条件测得面包酵母QN006的铁富集量为13.92mg/g干菌体,不加糖面团发酵力达到623mL/h。     

Cultivating the marine microalga Nannochloropsis salina under various nitrogen sources: Effect on biovolume yields,lipid content and composition,and invasive organisms

Algae can be a viable source for biofuel production, but the source of nitrogen used to cultivate could affect algae yields. Here, we observe how various nitrogen treatments can impact the growth and biovolume of microalga Nannochloropsis salina as well as invasion of undesired organisms. Invading organisms increase the likelihood of crashes of the desired microalgae culture. Experiments were conducted over 28 days in open aquaria in a greenhouse. We used five different nitrogen treatments; ammonium chloride (NH₄Cl), ammonium hydroxide (NH₄OH), sodium nitrate (NaNO₃), urea (CH₄N₂O), and a mixture of all these sources. Highest values for Maximum Sustainable Yield (MSY), a measure of potential harvest rate based on population productivity, were observed in the urea treatment, but cell size was smaller compared to other treatments. Sodium nitrate and the mixture of nitrogen sources also had high MSY values but larger cell sizes, making them the treatments with highest total biovolume. The highest percentages of lipid by weight, but also highest densities of invading organisms were observed in the mixed treatment. Our results suggest that tradeoffs between biovolume and lipid yields as well as culture success can ultimately decide what nitrogen sources to use.     

Integrated LaB6/g-C3N4 solar absorber for solving dye accumulation during solar steam generation

Solar steam generation has attracted considerable interest due to its easy accessibility and sustainability. However, dye molecules were gradually concentrated on bulk water or the surface of solar absorbers during the disposal of dye wastewater. Herein, LaB₆/g-C₃N₄ composites were immobilized on porous cotton cloth, served as a solar absorber resistant to dye clogging. The optimal solar absorber possessed solar harvesting of 92.3% and showed great application potential in the field of the treatment of dye wastewater. This study presented a new approach for the treatment of dye wastewater.     

Bio-accumulation of metals on reef associated organisms of Lakshadweep Archipelago

Tissue samples of marine organisms from the coastal waters of Agatti Island were subjected to analysis of metals (cadmium, cobalt, copper, iron, magnesium, manganese, nickel, lead and zinc), using Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES) for the assessment of the present condition of the Island ecosystem and compiling the baseline data for future monitoring, with respect of metal accumulation of marine organisms. Tissue samples of fish, shellfish and seaweed revealed that the metals have different levels of accumulation viz. Cd = 0.08-0.14, Co = 0.01-0.02, Cu = 0.16-0.98, Fe = 3.19-5.3, Mg = 86.73-152.45, Mn = 0.17-0.55, Ni = 0.06-0.26, Pb = 0.11-0.46 and Zn = 3.26-14.2 μg g⁻¹ dry wt. Metal concentrations were more in shellfish and less in finfish. Concentrations of toxic metals such as Cd, Co, Ni and Pb were well below the permissible limits proposed by the World Health Organization.     

Effects of Different Oil Sources and Residues on Biomass and Metabolite Production by Yarrowia lipolytica YB 423-12

Yarrowia lipolytica is known to have the ability to assimilate hydrophobic substrates like triglycerides, fats, and oils, and to produce single-cell oils, lipases, and organic acids. The aim of the present study was to investigate the effects of different oil sources (borage, canola, sesame, Echium, and trout oils) and oil industry residues (olive pomace oil, hazelnut oil press cake, and sunflower seed oil cake) on the growth, lipid accumulation, and lipase and citric acid production by Y. lipolytica YB 423-12. The maximum biomass and lipid accumulation were observed with linseed oil. Among the tested oil sources and oil industry residues, hazelnut oil press cake was the best medium for lipase production. The Y. lipolytica YB 423-12 strain produced 12.32 ± 1.54 U/mL (lipase activity) of lipase on hazelnut oil press cake medium supplemented with glucose. The best substrate for citric acid production was found to be borage oil, with an output of 5.34 ± 0.94 g/L. The biotechnological production of valuable metabolites such as single-cell oil, lipase, and citric acid could be achieved by using these wastes and low-cost substrates with this strain. Furthermore, the cost of the bio-process could also be significantly reduced by the utilization of various low-cost raw materials, residues, wastes, and renewable resources as substrates for this yeast.     

The effect of biomass co-combustion in a CFB boiler on solids accumulation on surfaces of P91 steel tube samples

The paper describes the results of industrial tests associated with the co-combustion of biomass and agromass with coal and focused on diagnostics and assessment of the effect of fuel type on the accumulation of solids on the surface of P91 steel tube samples placed in the vicinity of steam superheater in a commercial large-scale (over 400 MW) circulating fluidized bed boiler.Analysis of samples indicated the effect of fuel type, as well as the location of the area on the steel tube on the rate of solids accumulation. Alkali compounds were quite uniformly distributed at both front and rear sections of the steel tube samples, while calcium and sulfur components were mainly found in the front area. The average solids accumulation rates calculated for the rear areas of P91 steel tube samples were roughly two times higher than those for the front ones. The highest solids accumulation rate on the front area (1.04·10⁻⁹ ms⁻¹) was calculated for the case of agromass co-combustion with coal.     

Modelling Gas Transmission in Cylindrical Dynamic Accumulation Oxygen Transmission Rate Chambers to Explore Implications of Oxygen Sensor Location Relative to Samples

A model was developed to simulate oxygen accumulation in space and time within cylindrical dynamic accumulation chambers that are used to measure oxygen transmission rate (OTR) of materials. The model is based on Fick's law of diffusion and was validated against actual OTR measurements of polymer film samples. Measured OTR values and thicknesses were inputted into the model and oxygen concentrations outputted by the model. OTRs determined from the output of the model was in close agreement to within 0.3–3% of the measured OTR. Oxygen concentration versus time curves generated from model output oxygen concentrations and experimentally measured oxygen concentrations for three actual films were also in agreement. The model was then used to simulate results from three hypothetical test films at varying chamber lengths in order to evaluate effects of accumulation chamber dimensions relative to films on resulting OTR measurements. A typical design scenario was used, where the oxygen sensor is mounted on the chamber wall opposite the sample film. Results demonstrate that dynamic accumulation OTR instrument designers have considerable flexibility in choosing accumulation chamber dimensions because deviations in OTR are only expected to occur at impractically extreme chamber lengths (>10 m) for the entire envelope of OTRs expected for typical packaging films. Copyright © 2014 John Wiley & Sons, Ltd.     

一种基于北斗信号的星载双站SAR成像频率同步的方法

针对基于北斗信号的星载双站SAR成像频率同步误差,提出一种在高噪声情况下新的频率同步方法。以北斗卫星直达波信号为同步信号源建立同步通道,使用单周期积累的扩频信号优化时频捕获算法得到较精确伪码时延,在码同步后使用已知时延的多周期积累的时频捕获算法获到频率误差。连续10次计算机仿真在SNR=-50db的高噪声环境下频率误差均不超过1.5Hz。