Model selection, identification and validation in anaerobic digestion: a review

Anaerobic digestion enables waste (water) treatment and energy production in the form of biogas. The successful implementation of this process has lead to an increasing interest worldwide. However, anaerobic digestion is a complex biological process, where hundreds of microbial populations are involved, and whose start-up and operation are delicate issues. In order to better understand the process dynamics and to optimize the operating conditions, the availability of dynamic models is of paramount importance. Such models have to be inferred from prior knowledge and experimental data collected from real plants. Modeling and parameter identification are vast subjects, offering a realm of approaches and methods, which can be difficult to fully understand by scientists and engineers dedicated to the plant operation and improvements. This review article discusses existing modeling frameworks and methodologies for parameter estimation and model validation in the field of anaerobic digestion processes. The point of view is pragmatic, intentionally focusing on simple but efficient methods.     

Phosphorus concentrations in the River Dun, the Kennet and Avon Canal and the River Kennet, southern England

Variations in phosphorus (P) concentrations in an agriculturally impacted river draining a Chalk aquifer and an associated canal in the west of the Thames Basin, southern England are examined and linked to agricultural and sewage sources and within river/canal process controls. The study area comprises the River Dun, the adjacent River Kennet and the Kennet and Avon (K&A) Canal. Large seasonal variations are observed for soluble reactive phosphorus (SRP) and dissolved silicon (Si) with low concentrations in the spring and summer times when biological activity is high. The K&A Canal shows the largest SRP and Si concentration declines. This reflects high biological activity coupled with higher temperatures and higher water residence times. The extent of SRP removal is examined in relation to organic (uptake/release with phytoplankton growth/decay) and, to a lesser extent, inorganic (SRP coprecipitation with calcite) mechanisms. Boron (B) is used as a tracer of sewage sources. Agricultural inputs of both dissolved and particulate P (PP) can be important particularly under conditions where the catchment is wet and near surface/overland flow is important: sewage treatment works effluent and septic tank discharges to groundwater also probably provide a major component of the SRP occurring within the water column. The canal, and to a lesser extent the river, acts as sink for P in sewage effluent sources due to the high biological activity especially during the spring and summer. The aquifer probably acts as a major sink for agricultural and septic tank inputs of P.     

Effects of iron surface pretreatment on sorption and reduction kinetics of trichloroethylene in a closed batch system

The decline of trichloroethylene (TCE) in a metallic iron-water system results from the combination of reduction reaction and sorption onto iron surfaces. Sorption, particularly by highly impure iron, accelerates the removal of TCE from the aqueous phase, but delays the prevalence of steady-state conditions. In this case, an overly high value of reaction rate constant in the design of a treatment system would be used. In this work, the effects of an iron surface with 8.0% C, 6.1% O and 0.8% Si separately following HCl-washing and H2-reducing pretreatment on sorption and reduction rates were examined. The amounts of both aqueous and sorbed TCE were measured using a modified solvent-extraction method. TCE sorption onto an iron surface, as quantified by the Langmuir sorption maximum, followed the trend H2-reduced Fe0 > HCl-washed Fe0 > untreated Fe0 (0.887, 0.365 and 0.311 mg/g, respectively). Measurements of the concentration of sorbed TCE revealed that about 34-37% of the initial mass of TCE in the aqueous phase was removed by sorption by H2-reduced Fe0, 16-19% was removed by HCl-washed Fe0 and 13-16% was removed by untreated Fe0. A combination of new and previously reported data on cast iron's capacity to sorb TCE revealed a linear relationship between this capacity and the C fraction in the surface of the iron, with the coefficient of determination (r2) exceeding 0.99. The first-order observed rate constants (k(obs)) of the reduction of TCE in contact with Fe0 were obtained from the slope of a plot of total TCE loss rate (-dC(T)/dt) versus the amount of TCE in the aqueous phase (C(w)) using linear least-squares analysis. The k(obs) values were 0.080, 0.148 and 0.191 h(-1) for untreated, HCl-washed and H2-reduced Fe0, respectively. Normalized to iron surface area concentration, the specific rate constants (k(SA)) were 2.3 7x 10(-3) , 2.31 x 10(-3) and 5.62 x 10(-3) h(-1) m(-2) L, respectively. The results indicated that HCl-washing approximately doubled k(obs), primarily because of the increase in the surface area of the iron, and it slightly decreased k(SA) due to rapid corrosion during the rinsing process. Both the number of reactive sites and the sorption capacity per unit iron surface area through the H2-reducing pretreatment were increased due to the reduction of iron oxide layer and the carbonization of carbon-containing subjects on the iron's surface. Hence, the H2 reduction of cast iron promotes the removal of TCE from contaminated water by the concurrent sorption and reduction.     

Molecular subtypes of Campylobacter spp., Salmonella enterica, and Escherichia coli O157:H7 isolated from faecal and surface water samples in the Oldman River watershed, Alberta, Canada

Campylobacter spp., Salmonella enterica, and Escherichia coli O157:H7 isolated from 898 faecal, 43 sewage, and 342 surface water samples from the Oldman River were characterized using bacterial subtyping methods in order to investigate potential sources of contamination of the watershed. Among these pathogens, Campylobacter spp. were the most frequently isolated from faecal, sewage, and surface water samples (266/895, 11/43, and 91/342, respectively), followed by Salmonella (67/898, 8/43, and 29/342, respectively), and E. coli O157:H7 (16/898, 2/43, and 8/342, respectively). Salmonella Rubislaw was the most common serovar isolated from water. This serovar was also isolated from two wild bird species. Most other serovars isolated from water were either not isolated from animals or were isolated from multiple species. E. coli O157:H7 was predominantly isolated from cattle. The most common phage-types of this pathogen from cattle were also the most common among water isolates, and there were exact pulsed field gel electrophoresis and comparative genomic fingerprint matches between cattle, sewage, and water isolates. Campylobacters were commonly isolated from surface waters and faeces from most animal species. Restriction fragment length polymorphism of the Campylobacter flaA gene identified several location and host species-specific (cattle, goose, pig) fingerprints. Molecular subtyping of these bacterial pathogens shows considerable promise as a tool for determining the sources of faecal pollution of water.     

Evaluation of the migration of mutagens/carcinogens from PET bottles into mineral water by Tradescantia/micronuclei test,Comet assay on leukocytes and GC/MS

This study monitored the release of mutagenic/carcinogenic compounds into mineral water (natural and carbonated) from polyethylene terephthalate (PET) bottles, using a plant mutagenicity test which reveals micronuclei formation in Tradescantia pollen cells (Trad/MCN test), a DNA damage assay (Comet assay) on human leukocytes and gas chromatography/mass spectrometry (GC/MS) for the characterisation of migrants. The water samples were collected at a bottling plant and stored in PET bottles for a period ranging from 1 to 12 months. Every month some samples were randomly collected and lyophilised, the residual powders were extracted with organic solvents and then analysed by GC/MS and tested for DNA damage in human leukocytes, or reconstituted with distilled water to obtain concentrates for the exposure of Tradescantia inflorescences. Micronuclei increase in pollen was found only in natural mineral water stored for 2 months. DNA-damaging activity was found in many of the natural and carbonated water samples. Spring water was negative in the plant micronuclei test and the Comet assay, whereas distributed spring water showed DNA-damaging effects, suggesting a possible introduction of genotoxins through the distribution pipelines. GC/MS analysis showed the presence in mineral water of di(2-ethylhexyl)phthalate, a nongenotoxic hepatocarcinogenic plasticizer, after 9 months of storage in PET bottles.     

提高社会对核废处理的认识

一九五七年,一个从国家科学院—国家研究委员会挑选的地质学家和地球物理学家组成的委员会,提出了在洞室中隔离放射性废物的概念。在研究进行的初期阶段,研究和发展的方向基本上是在盐层中建造核废物隔离室。一九七○年到一九八○年的后五年中,美国、加拿大、瑞典和南德投入废物技术计划的财力和人力都有很大的增加,从而该计划的范围,也扩大到对包括在岗岩、玄武岩、混灰岩和页岩在内的地质介质的研究。那么,二十五年以后,放射性废物洞室地质隔离的原始概念,又将是一个什么样的状况呢?最重要的是,目前美国投入这项活动的资金,已经比一九七○年的金额大得多。根据放射性废物处理国内检查团所作的调查和介绍,卡特总统代表政府于一九八○年二月制订了一个全面的能源部废物处理计划。该计划主要要求在若干岩石类型各异的不同地质环境中,确定四到五个恰当的、具有潜力的隔离室建筑场地,并在一九八五年左右选择其中一个场地,     

Modeling the decay of ammonium oxidizing bacteria

A bench-scale sequencing batch reactor was used to study factors affecting the endogenous decay of the ammonium oxidizing biomass (AOB) in different operating conditions. AOB decay was very sensitive to oxygen concentration, and increased up to 0.4 d⁻¹ for oxygen concentration of 7 mg O₂ L⁻¹. The decay in anaerobic conditions was shown to be very low (0.03 d⁻¹) when compared to literature data.The effect of nitrite and nitrate on AOB decay was also studied. The correlation was quite weak suggesting that both nitrate and nitrite absence had little impact on decay which is contrary to what is typically assumed in some of the existing process models. A simple expression for the decay of AOB was proposed, calibrated and validated using the results of batch kinetic tests and of the continuous sequencing batch reactor monitoring.     

发人深思的堪萨斯城

引人注目的贴在很多墙上为1981年6月8～10日召开的美国地下空间会议和展览会(nSCE—81)所做的新广告是值得的,因为它准确地和形象地反映了砂岩的结构和颜色。然而其更恰当的是广告的标题——“想想地下”(THIHK EEP),它确切地号召我们在工作之前经常地想想地下,想想美国地下空间81年会议和展览会的全部目的。在全国范围内我们正处在个一进一步工业化和改变生活习惯的转变时期,作为始终的,我们应对将来作出聪明的决定,否则我们将会陷入无知识的行动面遗憾。现在是思考的时候     

为民防准备地下建筑物

最近议论的焦点,集中在对美苏间民防备战方面,令人不安的差距,这种议论给美国发展地下设备带来了新的活力,一旦受到核袭击,这些地下设备即可转为防核掩蔽所。据现在的估计,在一场核战争中,受害的美国居民将十倍于苏联。估计,如果苏联人来得及进入其广大的防护系统并执行其严密规划的城市疏散计划,则在全面相互核攻击中,他们的人口损     

Effect of different land use on soil erosion in the pre-alpine fringe (North-East Italy): Ion budget and sediment yield

Data concerning ion budget from rainfall, runoff and soil loss measurements from two experimental plots located in North-East Italy, under different land cover (beech forest and vineyard, respectively), are reported. The chemical composition of rainfall, soil solution and runoff was determined to study the solute cycle within the soil, and the relationships between water, soil and vegetation. The forest ecosystem is more prone to acidification than the agricultural one; the elemental concentration of runoff water is considerably lower than that of soil solution. Comparing the ion input (rainfall) and output (runoff) at the two sites, it was possible to estimate the ion budget in the two soil-vegetation systems. The runoff amount and the ion balance are related to rainfall volumes and composition; the measured runoff under vineyard is 59% in comparison to the one under beech. The soil loss at the two experimental sites (170 kg/ha and 132 kg/ha, respectively) is quite limited. The measured sediment yield of the two catchments (0.24-0.19 t/ha/year, respectively) is consistent with data reported for native forests of western Europe. Comparing rainfall and soil loss at the two sites proved that maintenance of some form of land cover is advisable if runoff and erosion are to be minimized. In the European context, where the most important consequences of erosion are sedimentation downstream and loss of productivity, this type of data will yield valuable information for the understanding of such processes occurring at catchment scale, and will help policy-makers develop appropriate programs for the territory safeguard.