Flow Cytometry: From Experimental Design to Its Application in the Diagnosis and Monitoring of Respiratory Diseases

Recent advances in the field of flow cytometry (FCM) have highlighted the importance of incorporating it as a basic analysis tool in laboratories. FCM not only allows the identification of cell subpopulations by detecting the expression of molecules in the cell membrane or cytoplasm, but it can also quantify and identify soluble molecules. The proper functioning of the FCM requires six fundamental systems, from those related to the transport of events to the systems dedicated to the analysis of information. In this review, we have identified the main considerations that every FCM user must know for an optimal antibody panel design, the quality systems that must govern the FCM protocols to guarantee reproducible results in research or clinical laboratories. Finally, we have introduced the current evidence that highlights the relevance of FCM in the investigation and clinical diagnosis of respiratory diseases, establishing important advances in the basic and clinical study of diseases as old as Tuberculosis along with the recent proposals for the monitoring and classification of patients infected with the new SARS-CoV2 virus.     

枞树型核电轮槽精铣刀制造技术优化设计

以枞树型叶根精铣刀为例,阐述了可换硬质合金型线刀体和刀片精铣刀的优化设计、制造工艺、建模及优化设计,进行切削过程加工策略优化和加工过程模拟,设计制造了锥柄专用工装及检测环规、磨削刀片后角专用工装,采用NUMROTO软件编制刀片加工工步。该项目研制的可换刀片精铣刀型线精度完全能达到进口铣刀水平,能满足大部分轮槽与叶根加工对型线精度的要求,可以大幅度节约刀具费用。     

Synthesis of colloidal nanosilica from waste glass powder as a low cost precursor

Waste glass powder was used as a low cost precursor for production of colloidal nanosilica for the first time. The process includes production of wet silica gel and thermal peptization of the wet gel. Purification of the glass powder and wet gel production were initiated by acid washing. The obtained powder was reacted with sodium hydroxide to produce wet silica gel. Type of the applied acid was examined in one factor at a time route. Temperature of the alkaline step and concentrations of the applied acid and base were investigated using Taguchi design of experiments. After finding the best combination of the investigated factor levels in production of the wet gel, time of the stabilization in thermal peptization was studied. Characterizations of the wet gel and colloidal silica were performed by XRF, DLS, FESEM, TEM, FTIR and N₂ sorption evaluation. Accordingly pure and stable colloidal nanosilica (98.50%) with average particle size of 21.9?nm was produced from the glass powder successfully. Specific surface area of the dried porous optimum sample was 83.63?m²/g.     

Patient involvement in Danish hospital design

This paper examines patient-involvement in the process used to design new hospitals. Denmark is in the midst of a phase of hospital building. This recent experience is studied by means of expert interviews with senior practitioners involved in three projects. Examined thematically, the data covers the structure of the design process, identification and ranking of stakeholders, the methods of user-involvement and approaches to accessibility. The paper adds new insight on an under-studied area, design for hospitals and makes recommendations for a change of approach to user-participation in their design. It highlights the difficulties in applying the methods of user-centred design and Design for All in large projects.     

深水水下管汇的结构设计概述

以南海某油气田的深水水下管汇为例,结合管汇的功能需求、结构组成、结构计算等,对深水水下管汇的结构设计进行了概述。     

基于CFD-DEM方法的净化器流场模拟与结构优化

针对空气净化器能耗高的问题，使用离散元方法(DEM)在吸附滤网中建立随机堆积柱形活性炭模型，采用计算流体力学(CFD)方法对空气净化器内部流场进行数值模拟，在模拟与实验验证的基础上，考察了压降最小、流场最均匀的吸附滤网结构。结果表明，空气净化器压降主要发生在轴向，活性炭吸附滤网中回流、沟流现象严重，流体阻力是其他两种滤网的3倍。边数对多边形填充孔结构吸附滤网内压降与流场均匀性无影响，当孔结构改为圆形时，压降减小约52 Pa，节能18.4%(49 W)；当孔直径由8 mm增至12 mm，压降减小约48 Pa，节能19.4%(45 W)；滤网间距对空气净化器压降无影响，圆形、小孔径的吸附滤网内流场最均匀。     

实用型气动旋转装夹工作台的设计

该设计实例为一种气动旋转装夹工作台,详细介绍该装置的设计思路,分析其结构和动作原理。该装置在实际应用中,完全满足了特殊工况需求,性能稳定,具有一定的推广价值。     

大块煤破碎装置的设计与应用

岳城煤矿2135厚煤层工作面在开采实践过程中,片帮大块煤会造成带式输送机的堵塞,引发运输故障,需要停机检修。基于该问题设计了一种对大块煤进行有效破碎的装置,应用中设备操作简便,有效地解决了带式输送机机头卸载点和转载机入口的堵塞问题,为企业创造了较好的经济效益。     

高等级生物安全实验室通风空调系统冗余设计原则探讨

对比分析了国内外标准有关高等级生物安全实验室通风空调系统冗余设计的要求。结合我国已有高等级生物安全实验室,重点分析了送风机、排风机、排风高效过滤器冗余设计的具体实施形式和存在的问题,给出了冗余设计原则建议。