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61.
62.
建立了乳珍中纳他霉素的高效液相色谱测定方法,原国标方法1GB/T21915-2008中,测定乳珍的回收率不到80%,并对该方法进行了改进,改进后回收率在95%以上,检出限在0.5mg/kg,线性范围0.5~20μg/ml。该方法操作简单,重现性好。 相似文献
63.
Characterization and osteoblast-like cell compatibility of porous scaffolds: bovine hydroxyapatite and novel hydroxyapatite artificial bone 总被引:5,自引:0,他引:5
Gao Y Cao WL Wang XY Gong YD Tian JM Zhao NM Zhang XF 《Journal of materials science. Materials in medicine》2006,17(9):815-823
Three different porous scaffolds were tested. The first two were prepared by sintering bovine bone. The third scaffold was
prepared using three-dimensional gel-lamination, a new rapid prototyping method, and was named as hydroxyapatite artificial
bone.
X-ray diffraction and Fourier transform infrared spectroscopy analysis confirmed that the samples were mainly highly crystalline
hydroxyapatite ceramics. Scanning electron microscopy and mercury intrusion porosimetry measurement showed that the pores
were interconnected and pore sizes ranged from several microns to hundreds of microns.
Mouse osteoblast-like cells grown on the three scaffolds retained their characteristic morphology. Cell proliferation and
differentiation, analyzed by methylthiazol tetrazolium (MTT) and alkaline phosphatase activity assays, were significantly
higher on the hydroxyapatite artificial bone than on the other two scaffolds tested. All the scaffolds provided good attachment,
proliferation and differentiation of bone cells.
These results indicate that the scaffolds have a favorable interaction with cells, they support cell growth and functions,
and therefore these scaffolds may have great potential as bone substitutes. The three-dimensional gel-lamination method is
proven to be an attractive process to design and fabricate bone scaffolds with favorable properties, and therefore, has promising
potential for bone repair applications. 相似文献
64.
65.
Tu JF Huang XF Hu RY You HY Zheng XF Jiang FZ 《World journal of gastroenterology : WJG》2011,17(38):4339-4343
AIM:To investigate the feasibility and therapeutic effect of laparoscopic surgery for pyogenic liver abscess(PLA) with biliary pathology.METHODS:From January 2004 to October 2010,31 patients with PLA combined with biliary pathology meeting entry criteria received surgical management in our hospital.Of the 31 patients,13 underwent laparoscopic surgery(LS group) and 18 underwent open surgery(OS group).Clinical data including operation time,intraoperative blood loss,postoperative complication rate,length of po... 相似文献
66.
生物活性肽是一类对生物机体有益且易被机体吸收的小分子聚合物, 是由2~20个氨基酸组成的蛋白质片段, 其功能性质由氨基酸组成和排列顺序决定。植物是生物活性肽的天然宝库, 植物源生物活性肽种类丰富、结构新颖, 是近年来的研究热点。生物活性肽被视为营养保健品和功能食品成分, 除了含有丰富的营养价值之外, 还具有抗氧化、降血压、抑菌、降血脂和维持血糖水平平衡等功能, 可利用其功能治疗、控制和预防慢性疾病, 对食品、医药和美容等领域的发展具有重要意义。本文综述了近5年从植物中获取生物活性肽的最新进展, 系统地总结了抗氧化肽、降压肽、抗菌肽、降血糖肽和降血脂肽的作用机制, 与目前最常见的健康问题进行对应说明。对生物活性肽的制备方法、分离纯化和鉴定技术进行总结比较, 并对生物活性肽的未来发展趋势以及在实际应用中遇到的问题进行展望, 为未来植物源生物活性肽的进一步研究开发与应用提供参考。 相似文献
67.
Kang YM Zhang AQ Zhao XF Cardinale JP Elks C Cao XM Zhang ZW Francis J 《Basic research in cardiology》2011,106(3):473-483
Recent studies indicate that systemic administration of tumor necrosis factor (TNF)-α induces increases in corticotrophin
releasing hormone (CRH) and CRH type 1 receptors in the hypothalamic paraventricular nucleus (PVN). In this study, we explored
the hypothesis that CRH in the PVN contributes to sympathoexcitation via interaction with neurotransmitters in heart failure
(HF). Sprague–Dawley rats with HF or sham-operated controls (SHAM) were treated for 4 weeks with a continuous bilateral PVN
infusion of the selective CRH-R1 antagonist NBI-27914 or vehicle. Rats with HF had higher levels of glutamate, norepinephrine
(NE) and tyrosine hydroxylase (TH), and lower levels of gamma-aminobutyric acid (GABA) and the 67-kDa isoform of glutamate
decarboxylase (GAD67) in the PVN when compared to SHAM rats. Plasma levels of cytokines, NE, ACTH and renal sympathetic nerve
activity (RSNA) were increased in HF rats. Bilateral PVN infusions of NBI-27914 attenuated the decreases in PVN GABA and GAD67,
and the increases in RSNA, ACTH and PVN glutamate, NE and TH observed in HF rats. These findings suggest that CRH in the PVN
modulates neurotransmitters and contributes to sympathoexcitation in rats with ischemia-induced HF. 相似文献
68.
69.
目的分析鼠李糖乳杆菌(Lactobacillus rhamnosus GG,LGG)对黄曲霉毒素B_1(aflatoxin B_1,AFB_1)的降解能力,阐述LGG对由AFB_1引起的肝损伤的保护作用。方法利用LGG培养液、LGG上清液组、LGG菌体、热处理后上清液和热处理后LGG菌体处理AFB_1,利用高效液相色谱法测定AFB_1的残留量,分析LGG对AFB的降解能力。利用低、中和高剂量的LGG菌液灌胃由AFB_1引起肝损伤的大鼠,并以空白和阳性作为对照组,测定大鼠的血清肝功能及肝组织抗氧化指标,分析LGG对肝损伤的保护作用。结果 AFB_1降解实验表明LGG菌液对AFB_1具有显著的降解作用(P0.05),36 h能够降解(92.01±2.02)%的AFB_1。降解作用是由菌体和LGG的代谢产物共同作用的结果。大鼠血清肝功能及肝组织抗氧化指标结果表明低、中和高剂量的LGG灌胃给药均能显著改善因AFB_1引起的大鼠肝功能和肝组织抗氧化指标异常(P0.05)。结论 LGG对AFB_1具有良好的降解作用,且能够有效抑制因AFB_1引起的肝损伤。 相似文献
70.
目的确定浙江红茶化学成分与感官品质关系。方法以龙井43和迎霜春梢一芽二叶为原料,采用不同萎凋工艺处理的红茶,通过主要化学成分测定和感官品质鉴定,结合通径分析,探究红茶主要化学成分与感官品质的关系。结果茶多酚及其衍生物之间与红茶感官品质之间存在一定相关性,表现相对较弱;茶多酚(X_1)、游离氨基酸(X_2)、茶黄素双没食子酸酯(theaflavin-3,3′-digallate,TFDG,X_4)、表儿茶素(epicatechin,EC,X_7)、表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG,X_8)和表儿茶素没食子酸酯(epicatechin gallate,ECG,X_9)与感官品质呈负相关,茶黄素(theaflavin,TF1,X__3)、茶黄素-3-没食子酸酯(theaflavin-3-gallate,TF-3-G,X_5)和茶黄素-3′-没食子酸酯(theaflavin-3′-gallate,TF-3'-G,X_6)与感官品质呈正相关,X_1、X__3、X__4、X__5和X__7通过X_6间接作用的通径系数均在1.3以上,远远高于其他间接作用的通径系数。结论影响浙江龙井43和迎霜所制的春季红茶品质的最主要成分是茶黄素单体TF-3'-G。 相似文献