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排序方式: 共有4208条查询结果,搜索用时 15 毫秒
71.
Tingjie Huang Qiang Yuan Shenghao Zuo Caijun Shi 《Journal of the American Ceramic Society》2020,103(11):6490-6504
Elastic behavior is an important aspect of fresh cement-based materials. In this paper, the elasticity development of fresh pure alite paste within 5 hours was monitored in situ by small amplitude oscillation shear. Isothermal microcalorimetry, X-ray diffraction, scanning electronic microscopy, 1H nuclear magnetic resonance, zeta potential measurements were used to investigate the underlying mechanisms. It was found that the evolution of storage modulus can be characterized by three different stages. The first stage stems from the compression of electrical double layer and the slight increase of solid volume fraction; the second stage is only controlled by the colloidal interaction; and the third stage is associated to the significant growth of solid volume fraction, the enhancement of short-range attraction and the rigidification of calcium silicate hydrate (CSH) network. Moreover, the relationship between the formation of CSH and the growth of elasticity was also quantified. 相似文献
72.
73.
Donatella Restuccia Gianluca Giorgi Umile Gianfranco Spizzirri Fabio Sciubba Giorgio Capuani Vittoria Rago Gabriele Carullo Francesca Aiello 《International Journal of Food Science & Technology》2019,54(4):1313-1320
Seeds and skins from grape pomaces of Pecorello and Mantonico cv underwent extraction (ultrasound–assisted or maceration), in order to obtain added-value ingredients for the production of a functional pear jam. The antioxidant features of the extracts were tested by in vitro colorimetric assays. Among seeds, Mantonico by maceration (MSC) showed the best results, as well as Mantonico by ultrasound-assisted extraction (MBs) among skin extracts. The selected extracts were fully characterised by NMR and MS techniques, confirming the presence of many polyphenols, flavonoids and tannins among others. Pectin was then derivatised by the grafting procedure with the active molecules of MBs and MSC. The latter produced the best antioxidant polymer also without toxicity evaluated using Caco-2 cells and was used for the jam preparation. The functional pear jam showed improved antioxidant performances in comparison with its non-functional counterparts as well as its maintenance over time (15 days). 相似文献
74.
Jia-Liang Chen Xiao Wang Yu-Hao Xiao Xun-Cheng Su 《Chembiochem : a European journal of chemical biology》2019,20(21):2738-2742
Unstable and low-abundance protein complexes represent a large family of transient protein complexes that are difficult to characterize, even by means of high-resolution NMR spectroscopy. A method to assign the NMR signals of these unstable complexes through a combination of selective isotope labeling of amino acids in a protein and site-specific labeling the protein with a paramagnetic tag is presented herein. By using this method, the resonances of unstable thioester intermediate complex (lifetime <5 h and highest concentration ≈20 μm ) generated by Staphylococcus aureus sortase A and its peptide substrate under a real-time reaction have been assigned. 相似文献
75.
Transverse relaxation times (T2) and T2 spectrum for crude oil emulsions with different water content are obtained by low field nuclear magnetic resonance Carr-Purcell-Meiboom-Gill sequence and various methods to determine the water content are investigated. The results show that there are three methods to accurately determine the water content. When water content is greater than 20.0wt%, determination through T2 value of emulsions is fastest with the relative error below 2.5%. T2 spectrum show the characteristics of multiple peaks, and T2 value of the peaks originated from water are suitable for determination of water content for O/W emulsions. A method is developed and optimized to determine the water content by using the peak-area ratio in T2 spectrum, which is not limited by the water content and the form of emulsion. 相似文献
76.
Takeshi Tenno Kohki Kataoka Natsuko Goda Hidekazu Hiroaki 《International journal of molecular sciences》2021,22(5)
Bioavailability is a major bottleneck in the clinical application of medium molecular weight therapeutics, including protein and peptide drugs. Paracellular transport of these molecules is hampered by intercellular tight junction (TJ) complexes. Therefore, safe chemical regulators for TJ loosening are desired. Here, we showed a potential application of select non-steroidal anti-inflammatory drugs (NSAIDs) as TJ modulators. Based on our previous observation that diclofenac and flufenamic acid directly bound various PDZ domains with a broad specificity, we applied solution nuclear magnetic resonance techniques to examine the interaction of other NSAIDs and the first PDZ domain (PDZ1) of zonula occludens (ZO)-1, ZO-1(PDZ1). Inhibition of ZO-1(PDZ1) is expected to provide loosening of the epithelial barrier function because the domain plays a crucial role in maintaining TJ integrity. Accordingly, diclofenac and indomethacin were found to decrease the subcellular localization of claudin (CLD)-2 but not occludin and ZO-1 at the apicolateral intercellular compartment of Madin–Darby canine kidney (MDCK) II cells. These NSAIDs exhibited 125–155% improved paracellular efflux of fluorescein isothiocyanate insulin for the Caco-2 cell monolayer. We propose that these NSAIDs can be repurposed as drug absorption enhancers for peptide drugs. 相似文献
77.
Benjamin Diethelm-Varela 《ChemMedChem》2021,16(5):725-742
Against the challenge of providing personalized cancer care, the development of targeted therapies stands as a promising approach. The discovery of these agents can benefit from fragment-based drug discovery (FBDD) methods that help guide ligand design and provide key structural information on the targets of interest. In particular, nuclear magnetic resonance spectroscopy is a promising biophysical tool in fragment discovery due to its detection capabilities and versatility. This review provides an overview of FBDD, describes the basis of NMR-based fragment screening, summarizes some exciting technical advances reported over the past decades, and closes with a discussion of selected case studies where this technique has been used as part of drug discovery campaigns to produce lead compounds towards the design of anti-cancer targeted therapies. 相似文献
78.
Xianlong Wang Chengfei Wang Hui Zhao 《International journal of molecular sciences》2012,13(11):15420-15446
Computational chemistry is an important tool for signal assignment of 27Al nuclear magnetic resonance spectra in order to elucidate the species of aluminum(III) in aqueous solutions. The accuracy of the popular theoretical models for computing the 27Al chemical shifts was evaluated by comparing the calculated and experimental chemical shifts in more than one hundred aluminum(III) complexes. In order to differentiate the error due to the chemical shielding tensor calculation from that due to the inadequacy of the molecular geometry prediction, single-crystal X-ray diffraction determined structures were used to build the isolated molecule models for calculating the chemical shifts. The results were compared with those obtained using the calculated geometries at the B3LYP/6-31G(d) level. The isotropic chemical shielding constants computed at different levels have strong linear correlations even though the absolute values differ in tens of ppm. The root-mean-square difference between the experimental chemical shifts and the calculated values is approximately 5 ppm for the calculations based on the X-ray structures, but more than 10 ppm for the calculations based on the computed geometries. The result indicates that the popular theoretical models are adequate in calculating the chemical shifts while an accurate molecular geometry is more critical. 相似文献
79.
An NMR-based metabolomic assessment of cultured cobia health in response to dietary manipulation 总被引:1,自引:0,他引:1
Commercial aquaculture feeds rely heavily on fishmeal and fish oil, which can be expensive and ecologically unsustainable. To evaluate the efficacy of reduced fishmeal diets for outgrowth, a dietary study was conducted on the finfish cobia, Rachycentron canadum. NMR-based metabolomic techniques were used to assess the effect of decreasing dietary fishmeal on the health of the cobia. Filtered serum 1H NMR spectra analysed by principal components analysis (PCA) showed cobia fed reduced fishmeal diets were metabolically different than cobia on control diets. In particular, tyrosine and betaine increased in cobia fed reduced fishmeal diets while glucose decreased, suggesting that these cobia were not receiving the necessary nutritional components required for energy and growth. The formulated control diet contributed to enriched growth and significantly elevated lactate levels suggesting enhanced gut microflora metabolism in response to dietary components. The results show that NMR-based metabolomic analysis is a useful tool in aquaculture studies. 相似文献
80.