Microbial fuel cell (MFC)-based biosensor for combined heavy metals monitoring and associated bioelectrochemical process

To explore the feasibility and related mechanism of MFC biosensor for wastewater detection under the action of combined heavy metals. Cyclic voltammetry (CV) and scanning electrochemical impedance spectroscopy (EIS) were used to explore the related bioelectrochemical process. The response of the reactor to single/combined heavy metals, low/high heavy metal concentrations, and the differences in ohmic resistance (Rs) and charge transfer resistance (Rct) were investigated using Ni as the core heavy metal and the combined action of Cd, Cu and Zn. The results indicated that there was a linear relationship between the concentration and output voltage of the MFC biosensor under the action of combined heavy metals (R² = 0.8803–0.973). However, the internal resistance (Rint) of the MFC biosensor under the action of single heavy metal was far less than that of the combined heavy metal group, and the power density (19.849 W m^?3) was 4 times that of the combined heavy metal group (3.109–4.589 W m^?3). The Rs of the biosensors in the combined heavy metal group were 0.868Ω and 0.860, which were higher than 0.768Ω of the single heavy metal sensor. With the increase of the concentration of heavy metals in the influent, the increase of Rct was more obvious in the combined group, while the Rs in the single group significantly increased (P < 0.05). The results imply that it is possible for MFC biosensors to be used in the detection of actual water polluted by various heavy metals, but the biosensor performance is mainly limited by Rct, which needs to be further improved.     

个性化学习路径推荐综述

近年来，伴随着现代信息技术的迅猛发展，以人工智能为代表的新兴技术在教育领域得到了广泛应用，引发了学习理念和方式的深刻变革.在这种大背景下，在线学习超越了时空的限制，为学习者“随时随地”学习提供了更多的可能性，从而得到了蓬勃发展.然而，在线学习中师生时间、空间分离的特征，导致教师无法及时掌握学生的学习状态，一定程度上制约了在线学习中教学质量的提升.面对多元化的学习需求及海量学习资源，如何迅速完成学习目标、降低学习成本、合理分配学习资源等问题成为限制个人和时代发展的重大问题.然而，传统的“一刀切”的教育模式已经不能满足人们获取知识的需求了，需要一个更高效、更科学的个性化教育模式，以帮助学习者以最小的学习成本最大限度地完成学习目标.基于以上背景，如何自动高效识别学习者特征，高效地组织和分配学习资源，为每一位学习者规划个性化路径，成为面向个体的精准化教育资源匹配机制研究中亟待解决的问题.系统地综述并分析了当前个性化学习路径推荐的研究现状，并从多学科领域的角度分析了对于同一问题的不同研究思路，同时也归纳总结了当前研究中最为主流的核心推荐算法.最后，强调当前研究存在的主要不足之处.     

水力空化改质对重质原油沥青质及性质的影响

利用水力空化过程产生局部的高温、高压、高射流以及强大的剪切力等极端化学物理条件改质处理沙特重质原油，试验结果表明：沙特重质原油经过水力空化改质后粘度由13.61降低至7.22mm2/s，残碳由7.16%降低至6.48%，实沸点蒸馏后减压渣油降低1个百分点。进一步采用APPI FT-IR MS、XRD、FT-IR、SEM和粒度分布等技术研究了水力空化改质对沙重原油分子组成，沥青质团聚体微晶结构、沥青质胶束粒径分布、沥青质官能团、沥青质形貌等方面的影响，从分子角度阐述空化改质重油的机理。研究结果表明：水力空化改质后沙重原油分子量分布、芳烃类化合物缔合作用变小；沥青质对低DBE化合物吸附性能降低；沥青质团聚体微晶结构更加松散；沥青质胶束粒度分布降低；沥青质分子相互团聚作用力减弱。进一步考察了水力空化改质前后减压渣油延迟焦化性能，改质处理后焦炭产率降低1.85个百分点，液体收率和气体产率分别增加1.52和0.33个百分点，水力空化改质对沥青质性质、结构特点的改善能够有效的提高其加工性能。     

下扬子地区下志留统风暴沉积特征及风暴作用对泥页岩品质的影响

近年来在下扬子地区页岩气地质调查井所获取的地层岩心资料，揭示该地区下志留统风暴沉积较为发育。基于精细的岩心观察描述，详细分析了下扬子地区下志留统高家边组下段中上部风暴沉积的特征，并阐明了风暴作用对泥页岩的影响。研究表明：研究区风暴岩岩性偏细，以粉砂岩为主，石英含量较高，结构成熟度较低，具2种不同的粒度概率曲线；主要发育底部冲刷面、块状层理、平行层理、丘状交错层理、波纹交错层理、准同生变形构造等沉积构造；垂向上主要识别出4种风暴沉积序列，均具有从底到顶粒度变细的沉积特征，不同的沉积序列与风暴流能量的强弱有关。下扬子地区早志留世频繁的风暴活动与研究区的古地理背景和古气候条件具有内在联系。特大型风暴对浅海陆棚深水区的细粒泥质沉积物特征产生重要影响，包括降低有机质含量、减少单层厚度、破坏垂向连续性、改变原始沉积构造和侧向稳定性等，总体上降低了泥页岩的品质。因此，研究认为在页岩气的勘探和评价过程中应重视风暴因素对陆棚深水区原始泥质沉积物的影响。     

基于人工神经网络的智能化架空线路应变快速解调方法北大核心

为了提高智能化光纤复合架空线路态势感知的实时性,将人工神经网络方法应用于光纤沿线应变解调,确定了神经网络的结构。编程实现了基于洛伦兹模型的最小二乘谱拟合方法和神经网络方法,采用不同信噪比和布里渊频移的布里渊谱训练神经网络,将它们应用于某光纤复合架空线路沿线光纤应变的测量,从不同角度比较了两种方法的计算结果。计算结果表明,神经网络方法能有效获得光纤沿线的布里渊频移进而获得应变,具有与谱拟合方法相似的准确性,但应变解调时间仅约为谱拟合方法的1/20000。研究结果为提高智能光纤复合架空线路态势感知的实时性提供了参考。     

具有保护肠上皮细胞HT-29免受大肠杆菌和H2O2损伤潜力的植物乳杆菌的筛选

益生菌可在肠道定植从而发挥抗炎或抗氧化活性，有利于宿主肠道健康。本实验研究了从新疆传统发酵乳制品中分离得到的8?株植物乳杆菌对大肠杆菌侵袭和过氧化氢刺激肠上皮细胞HT-29的保护作用。结果表明：在8?株植物乳杆菌中，植物乳杆菌35具有最高的黏附能力。植物乳杆菌35可通过取代、竞争、排阻的方式抑制大肠杆菌对HT-29细胞的黏附，抑制率分别为42.60%、59.17%、60.19%。植物乳杆菌35及其多糖可抑制大肠杆菌刺激HT-29细胞产生白细胞介素-8；同时保护HT-29细胞免受过氧化氢的损伤，增加超氧化物歧化酶、谷胱甘肽过氧化物酶活力水平并降低丙二醛含量。结论：植物乳杆菌35及其粗胞外多糖具有抑制大肠杆菌O157诱导的炎症性肠病的潜力。     

四氢姜黄素经PI3K/Akt信号通路对人血小板活化和聚集的调控作用

目的：探讨姜黄素的主要肠道代谢物四氢姜黄素（tetrahydrocurcumin，THC）对血小板活化和聚集的影响及其可能的分子机制。方法：在体外实验中，用不同浓度的THC（0、0.5、1、10 μmol/L）提前与健康人纯化血小板共同孵育40 min，然后加入凝血酶激活血小板2 min，用流式细胞术测定血小板表面CD62P和CD63的表达量，用酶联免疫吸附法测定血小板释放血小板因子-4（platelet factor-4，PF4）和趋化因子配体-5（chemokine ligand 5，CCL5）水平，用血小板聚集仪检测血小板释放ATP水平和血小板最大聚集率，用Western blot蛋白免疫印迹法检测血小板磷酸肌醇-3-激酶（phosphoinositide 3-kinase，PI3K）和Akt蛋白的磷酸化水平。结果：与模型组（血小板悬液中加入0.05%二甲基亚砜）相比，THC能抑制凝血酶诱导的血小板表面CD62P和CD63的表达，抑制PF4、CCL5和ATP的释放，降低血小板最大聚集率，下调PI3K和Akt蛋白的磷酸化水平，且呈浓度依赖效应，其中10 μmol/L的浓度下作用效果显著（P＜0.01、P＜0.001）。PI3K的特异性激动剂740 Y-P可部分逆转THC对PF4和CCL5释放和血小板聚集的抑制作用（P＜0.05、P＜0.01）。结论：THC具有显著抑制血小板活化和聚集的作用，其机制可能是THC可下调PI3K/Akt介导的信号通路。     

Ferulic acid and EGCG alter the structural characteristics of ovalbumin and its application in mineral loading

This study aims to fabricate mineral-loading nanocarriers using natural materials. The interaction patterns between ovalbumin (OVA) and four water-soluble polyphenols, namely ferulic acid (FA), (-)-Epigallo-catechin 3-gallate (EGCG), gallic acid (GA) and epicatechin (EC), were investigated. Results showed that the optimised conditions for preparing stable OVA–polyphenol complexes are at the OVA–polyphenol ratio of 4:1 at pH 6, under which OVA–FA and OVA–EGCG showed the highest stability and mineral-loading capacity among four OVA–polyphenol complexes. The fluorescence results indicated that the addition of EGCG and FA induced a significant fluorescence quenching to OVA. The interaction between OVA and polyphenols involved hydrogen bonding, hydrophobic interaction and electrostatic interaction. Fourier transform infrared spectroscopy (FTIR) analysis suggested that both FA and EGCG enhanced the stability and orderliness of the structure of OVA. The transmission electron microscopy images also exhibited the spherical structure of OVA after the addition of FA and EGCG. Furthermore, scanning electron microscope–energy dispersive X-ray spectrum results suggested that OVA–FA and OVA–EGCG complexes were better mineral carriers than OVA–GA and OVA–EC. This study may serve as the theoretical support for the promising application of OVA in the fabrication of mineral-loading nanocarriers in functional food and pharmaceutic.     

In-situ measurement of mineral phase transition and kinetics in roasting process of Bayan Obo mixed rare earth concentrate by sodium carbonate

In this study, the Bayan Obo rare earth concentrates mixed with Na₂CO₃ were used for roasting research. The phase change process of each firing stage was analyzed. The kinetic mechanism model of the continuous heating process was calculated. This study aims to recover valuable elements and optimize the production process to provide a certain theoretical basis. Using X-ray diffraction (XRD), Fourier infrared spectroscopy, scanning electron microscopy with energy dispersive spectrometry, the reaction process and the existence of mineral phases were analyzed. The variable temperature XRD and thermogravimetric method were used to calculate the roasting kinetics. The phase transition results show that carbonate-like substances first decompose into fine mineral particles, and CaO, MgO, and SiO₂ react to form silicates, causing hardening. Further, REPO₄ and NaF can directly generate CeF₃ and CeF₄ at high temperatures, and a part of CeF₄ and NaF forms a solid solution substance Na₃CeF₇. Rare earth oxides calcined at a high temperature of 750 °C were separated to produce Ce_0.6Nd_0.4O_1.8, Ce₄O₇, and LaPrO_3+x. Then, BaSO₄, Na₂CO₃, and Fe₂O₃ react to form barium ferrite BaFe₁₂O₁₉; the kinetic calculation results show that during the continuous heating process, the apparent activation energy E reaches the minimum in the entire reaction stage in the temperature range of 440–524 °C, and the reaction order n reaches the maximum, which indicates that the decomposition product REFO significantly impacts the reaction system and reduces the activation energy. The mechanism function is F(α) = [?ln (1?α)]^1/3. The reaction order n reaches the minimum in the temperature range of 680–757 °C, and the apparent activation energy E is large. The difficulty of the reaction increases during the final stage. The reaction mechanism function is F(α) = [1?(1?α)^1/3]². Observing the entire reaction stage, the step of controlling the reaction rate changes from random nucleation to three-dimensional diffusion (spherical symmetry).