Aptamer/Peptide-Functionalized Genome-Editing System for Effective Immune Restoration through Reversal of PD-L1-Mediated Cancer Immunosuppression

Effective reversal of tumor immunosuppression is of critical importance in cancer therapy. A multifunctional delivery vector that can effectively deliver CRISPR-Cas9 plasmid for β-catenin knockout to reverse tumor immunosuppression is constructed. The multi-functionalized delivery vector is decorated with aptamer-conjugated hyaluronic acid and peptide-conjugated hyaluronic acid to combine the tumor cell/nuclear targeting function of AS1411 with the cell penetrating/nuclear translocation function of TAT-NLS. Due to the significantly enhanced plasmid enrichment in malignant cell nuclei, the genome editing system can induce effective β-catenin knockout and suppress Wnt/β-catenin pathway, resulting in notably downregulated proteins involved in tumor progression and immunosuppression. Programmed death-ligand 1 (PD-L1) downregulation in edited tumor cells not only releases the PD-1/PD-L1 brake to improve the cancer killing capability of CD8⁺ T cells, but also enhances antitumor immune responses of immune cells. This provides a facile strategy to reverse tumor immunosuppression and to restore immunosurveillance and activate anti-tumor immunity.     

水平受荷桩的截面优化设计

运用有限单元法和梯度法 ,尝试性地对端承桩进行变截面优化设计 ,编制了相应程序 ;并以一人工挖孔灌注桩为例 ,计算比较截面优化前后的内力、挠度。计算结果表明 ,变截面桩可节省混凝土材料约 45 %。     

空军汉口新机场试验路段石灰改性膨胀土试验研究

空军汉口新机场试验路段石灰改性膨胀土（简称灰土）试验研究包括室内和现场试验研究。室内试验研究包括：天然膨胀土与击实膨胀土的基本物理特性和胀缩特性试验、灰土击实试验以及膨胀土掺石灰改性试验等。室内试验结果表明：击实膨胀土比天然膨胀土的膨胀潜势更大；在道面下一定范围内，填料不能采用膨胀土，而必须用灰土；石灰能有效地对场区内的膨胀土进行改性，最优石灰掺合比为6％～8％：不同灰土层的最大干密度与最佳含水量差异较大，现场施工填料不能混填。现场试验包括：碾压试验、压实灰土基本物理特性和胀缩特性试验、浸水载荷试验、测定路基回弹模量和回弹弯沉试验等。现场试验结果表明：在有效控制灰土的石灰掺量和含水量情况下，采用激振力为450kN的碾压机对松铺厚度为50和30cm的灰土进行碾压，分别需碾压8和6遍，路基压路度才能达到95％，表面沉降才趋于稳定；现场压实灰土的膨胀潜势很低，仍有明显失水收缩特性，在施工时应注意采取保水措施；压实灰土具有较高承载能力、强度特性和吸水稳定性。     

Nanostructured active chitosan-based films for food packaging applications: Effect of graphene stacks on mechanical properties

Bioactive food-preserving materials are based on the use of a natural antimicrobial compound loaded in a carrier material, which is able to trigger its release when requested and to modulate the rate of release, thus using either toxic or inhibitory properties against pathogens or bacteria due to food decomposition. In this study, the Schiff base formation for chitosan functionalization was achieved by the reaction of chitosan with cinnamaldehyde at different concentrations. Cinnamaldehyde is an aromatic α,β-unsaturated aldehyde, and the major component in essential oils from some cinnamon species. It has been shown to exert antimicrobial action against a large number of microorganisms including bacteria, yeasts, and mould. The formation of the Schiff base is reversible under suitable conditions, and this might allow the release of the active cinnamaldehyde from chitosan, used as the carrier. The reaction kinetics was investigated by means of rheological measurements, while infrared spectroscopy was used to assess the efficacy of the functionalization. The addition of nanometric graphene stacks to the cinnamaldehyde-functionalized chitosan films was evaluated with the aim to increase the mechanical properties of the film. Finally, the films were tested for antifungal properties with bread slices against a selected mould line.     

Direct measurement method for plumb line deviations

The effect of irregularities of the refractive index of a terrestrial layer of the atmosphere on the slope of the polarization plane of an electromagnetic wave is evaluated numerically. An optical range polarimeter is developed having a sensitivity of the order of 1″. The possibility is demonstrated of practical application of the polarization plane as a basis for direct measurement of the relative angular positions (deviations) of a plumb line at scattered points of the earth’s surface.     

圆锥摩擦轮传动在大型锻形筒体环缝焊接中的应用

大型锥形筒体环焊缝是在专用滚轮架上用自动埋弧焊来完成的.由于滚轮架传动的缺陷以及筒体制造上的误差,使得滚轮架的滚轮与锥形筒体之间摩擦传动产生异常,导致锥形筒体旋转不稳定,焊接质量不能满足技术要求.为提高焊接质量,分析了上述两个影响焊缝质量的因素,提出滚轮架的滚轮摆放应符合圆锥摩擦传动原理,增加了滚轮轮缘摩擦装置来弥补筒...     

三维往复摆动式喷丸机械手

针对大型回转件外壁的人工喷丸存在的质量和效率问题，设计了三维往复摆动式喷丸机械手，可有效保护操作人员的健康，大大提离喷丸效率和质量，同时也减少了人工喷丸作业壁。     

WuT3无血清培养上清中单克隆抗体纯化工艺的建立

目的对WuT3杂交瘤细胞生物反应器无血清培养上清中单克隆抗体进行分离纯化,建立中试分离纯化工艺。方法采用两步离子交换层析法结合硫酸铵盐析法分离纯化WuT3单抗。在通过小量试验优化纯化参数后,进行中量试验和中试放大。结果建立的纯化方法可以放大到中试规模,每批投料30000ml培养上清,可收获单抗达1.5g,单抗总回收率大于60%。经分离纯化后,单抗IgG纯度大于95%,采用免疫荧光法检测抗体活性合格。结论建立了一条生物反应器无血清培养杂交瘤细胞大规模分离纯化单抗的工艺路线。     

The effects of oxygenation aeration treatment on dissipation behaviors of tricaine mesylate in carp (Cyprinus carpio) muscle and water

Tricaine mesylate (MS-222) is one of the most used anesthetics in fish. It can be absorbed by the human body via food consumption, with related detriments to human health. In this study, a modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) method was developed for the determination of MS-222 in carp muscle and water samples using liquid chromatography-tandem mass spectrometry (LC-MS/MS). For cleanup procedure, multiplug filtration cleanup (m-PFC) method with n-Hexane delipidation was adopted. The extraction solvent, cleanup methods and sorbents were optimized. All method validation parameters were in the acceptable range. The dissipation behavior study was followed by the method development. Firstly, the anesthesia dose and time were optimized in application study. Secondly, carps were revived for different period of time with (experimental group) and without (control group) the oxygenation aeration treatment to compare the dissipation rate of MS-222. After being anesthetized for 6 h at 50 mg/L and 12 h of elimination, the concentrations of MS-222 in crap muscle and water of experimental group was lower than those of control group. After 36 h of elimination under oxygenation aeration, over 90% of MS-222 was dissipated in carp muscle. The results showed that the half-life of MS-222 in carp muscle was 6.2 h. The findings suggested that the commonly-used oxygenation aeration treatment in aquaculture production had potential effects in accelerating the dissipation of MS-222 in carp and water. In this study, three days of withdrawal period was recommended in carps after MS-222 administration under oxygenation aeration.     

美术专业学生英语学习动机的调查研究

关于美术专业学生的大学英语教学的相关调查与研究长期以来较为缺乏．根据Gardner外语学习动机的分类，采用问卷抽样调查80名美术专业学生的英语学习动机，发现其融入型动机不明显，而工具型动机占主导地位．深化英语教学改革，可以通过改革评价目标和内容、改进教学手段等措施从动机的角度开展．