Cell-Free Synthesis of Selenoproteins in High Yield and Purity for Selective Protein Tagging

The selenol group of selenocysteine is much more nucleophilic than the thiol group of cysteine. Selenocysteine residues in proteins thus offer reactive points for rapid post-translational modification. Herein, we show that selenoproteins can be expressed in high yield and purity by cell-free protein synthesis by global substitution of cysteine by selenocysteine. Complete alkylation of solvent-exposed selenocysteine residues was achieved in 10 minutes with 4-chloromethylene dipicolinic acid (4Cl-MDPA) under conditions that left cysteine residues unchanged even after overnight incubation. Gd^III−Gd^III distances measured by double electron–electron resonance (DEER) experiments of maltose binding protein (MBP) containing two selenocysteine residues tagged with 4Cl-MDPA-Gd^III were indistinguishable from Gd^III−Gd^III distances measured of MBP containing cysteine reacted with 4Br-MDPA tags.     

Characterization of a plasma polymer coating from an organophosphorus silane deposited at atmospheric pressure for fire-retardant purposes

Protective coatings from diethylphosphatoethyltriethoxysilane (DEPETS) have been deposited on different polymer substrates in a plasma discharge operated at atmospheric pressure. Plasma polymer chemistry and structure were characterized by means of Fourier transform infrared spectroscopy (FTIR), laser desorption ionization-mass spectrometry (LDI-MS), nuclear magnetic resonance (NMR) and scanning electron microscopy (SEM). A chemical structure of the plasma polymer has been proposed based on the coating molecular characterization. Coatings were deposited on polycarbonate (PC) and polyamide 6 (PA6) substrates. The flame retardant properties of coated substrate samples were assessed using cone calorimetry and compared to those of bare substrates. A significant increase in the time to ignition (TTI), up to +143%, was recorded after coating deposition due to the formation of a high-performance barrier layer at the surface of both polymer substrates.     

CO2驱沥青质沉积对致密储层的伤害机理——以鄂尔多斯盆地延长组长8储层为例

为了揭示CO2驱替过程中沥青质沉积对致密储层的伤害机理,文中基于岩心核磁共振T2谱测试原理,开展了CO2注入压力下的岩心驱替实验,研究了沥青质在岩心中的沉积特征,评价了沥青质沉积对储层的伤害程度。实验结果表明:沥青质沉积量和渗透率伤害率随着CO2注入压力的升高呈现先快速上升后趋于平缓的趋势;在CO2注入过程中,沥青质主要沉积在弛豫时间大于10 ms的大、中孔隙,导致大、中孔隙占比下降,微细、小孔隙占比增加,且随着CO2注入压力的升高,大、中孔隙占比下降幅度增大,微细、小孔隙占比上升幅度增大;此外,沥青质沉积会引起润湿性发生反转,随着沥青质沉积量的增加,润湿反转指数增大,岩心润湿性不断向强油湿方向转变。     

BaTiO3基Au纳米颗粒复合薄膜的制备及其光学性质研究

采用溶胶-凝胶（sol-gel）方法，成功的将高体积面分比的Au纳米颗粒复合到BaTiO3的非晶薄膜中，并对其光学性质进行了研究。用XRD、TEM、椭偏仪、吸收光谱、光克尔效应OKE（Optical Kerr Effcet）方法对薄膜进行了表征和测试。从吸收光谱观察到表面等离子体共振SPR（Surface Plasma Resonance)峰随热处理温度升高而红移的现象。光学非线性测试表明薄膜具有高的三阶非线性极化率Χ^(3)和超快的响应时间。     

多模石英光纤SBS相位共轭实验研究

采用芯径为100μm、长2m的无掺杂多模阶跃石英光纤,在1.06μm波段,研究了不同焦距下,光纤与耦合透镜间的距离以及泵浦能量对反射率和波形的影响。在不同的入射能量下,得到最大反射率为68.95%,脉宽压缩到入射光脉宽的1/3～1/2。     

流量比对等离子体基团分布和薄膜结构的影响

使用光强标定的发射光谱(AOES)测量了CHF3／C6H6混合气体的微波电子回旋共振(ECR)放电等离子体中基团的分布状态。实验发现随着CHF3流量的增加，成膜基团CF、CF2、CH等的相对密度增大，而刻蚀基团F的密度也会增加，从而使得a—C：F薄膜的沉积速率降低。同时红外吸收谱(IR)分析表明，在高CHF3流量下沉积的a—C：F薄膜中含有更高的C—F键成分。可见在a—C：F薄膜的制备中CHF3／(CHF3 C6H6)流量比是重要的控制参量。     

Noise-Assisted Signal Reception in Threshold Neuron

1IntroductionSometi mes,noise can i mprove signal transmission orsignal processing in a nonlinear system.This phe-nomenonis the so-called Stochastic Resonance(SR)[1].Most studies of SRinvolve asubthresholdsignal througha single nonlinear systemand the non…     

射频板条CO_2激光器并联谐振技术的研究

本文利用周期性网络模型计算了射频板条CO2激光器电极的纵向电压分布．探讨了并联谐振技术在板条器件中获得成功运用的原因．提出了利用并联谐振技术进一步提高电压分布均匀性的两个途径．     

一种新颖的表面等离子谐振效应光波导水质传感技术

光学表面等离子谐振（ＳＰＲ）效应是一种很有效的水质检测技术，经过详细的理论推导，同了ＳＰＲ反射光强的普遍适用公式。在纯水与受污染水两种情况下，根据一些已知参数计算出结果。由计算结果数据一出的曲线比较表明，ＳＰＲ效应在两种情况下有明显差别，这也正是ＳＰＲ效应用于水质检测的理论根据。