CRISPR/Cas生物传感器检测食源性病原体的研究进展

食源性病原体引起的食源性疾病严重威胁着人类的健康，灵敏且快速检测食源性病原体成为预防疾病发生的重要手段。生物传感器具有灵敏度高，无需富集即可实时定量，易于现场检测等优点。CRISPR/Cas系统与生物传感器结合可提高生物传感器检测的灵敏度和准确性。本文介绍CRISPR/Cas系统的分类及作用机制，回顾基于CRISPR/Cas的各种生物传感器检测食源性病原体的方法，包括荧光传感器、电化学传感器和侧流层析试纸，分析应用不同Cas蛋白检测的优缺点，为食源性病原体的检测提供借鉴。     

Effects of LaCl_3 on photosynthesis and the accumulation of tanshinones and salvianolic acids in Salvia miltiorrhiza seedlings

The effects of LaCl3 on the growth,photosynthetic gas-exchange characteristics,chlorophyll fluorescence,and the accumulation of tanshinones and salvianolic acids in Salvia miltiorrhiza seedlings were investigated. The results showed that the increase in photosynthesis induced by LaCl3 might be attributed to the enhanced stomatal conductance of the leaves and the increased level of the photochemical efficiency of PS II. The accumulation of tanshinone IIA and cryptotanshinone was markedly increased with the application of LaCl3 at 20 and 60 mg/L,while tanshinone I was only slightly increased. The content of salvianolic acid B was,however,decreased with the treatment of LaCl3 at 200 mg/L.     

中药区划研究进展

摘 要 在分析过去中药区划研究工作的基础上,对中药区划的类型和研究方法进行了归纳和总结。中药 区划类型,按照区划对象可分为中药资源区划和外部环境因素区划。中药资源区划按照区划地域的大小可 分为全国中药资源区划和地区中药资源区划,外部环境因素区划按照区划要素的构成可划分为中药生态区 划和中药生产区划。中药区划方法包括:定性描述法、模糊数学方法、构建模板法、构建模型法和基于遥 感图像的区划方法。