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采用同位素内标气相色谱-质谱(GC-MS)联用法,快速测定调味品中3-氯-1,2-丙二醇(3-MCPD)的含量。试样中加入3-MCPD的氘代同位素作为内标,过柱,用正己烷淋洗,乙酸乙酯洗脱,经氮吹浓缩后采用七氟丁酰咪唑(HFBI)进行衍生化,采用GC-MS选择离子监测(SIM)模式进行定性定量分析。结果表明,本方法的添加回收率≥80%;相对标准偏差≤7%;检出限达到5.0μg/kg。本方法步骤简便,溶剂用量少,定性定量准确可靠。 相似文献
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为让调味品生产企业和消费者进一步认识、研发川菜风味的调味品,从调味品在川菜中的选择,调味的手段及成菜风味等3个方面说明并阐述其应用.根据社会发展所需,认为调味品在川菜中应用的趋势是大量使用复合调味品进行分段调味. 相似文献
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Gbenga Adedeji Adewumi Wahengbam Romi Thangjam Anand Singh Kumaraswamy Jeyaram 《Food Biotechnology》2014,28(3):250-268
Phylogenetic relationship and strains sub-typing of Bacillus species isolated from iru, a traditional fermented condiment in Africa were studied using polyphasic genomic approaches and the profiles compared with bacilli isolated from similar Asian condiments. The 16S rRNA gene sequencing identified the strains as Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus licheniformis, Bacillus pumilus, and Brevibacillus formosus. The phylogenetic analysis conducted showed five distinct clusters with genetic relatedness among B. subtilis and B. amyloliquefaciens strains from Africa and Asia. Amplified ribosomal DNA restriction analysis (ARDRA) successfully differentiated species of B. subtilis phylogeny from B. cereus. Combined analyses of ARDRA, internal transcribed spacer-polymerase chain reaction (ITS-PCR), ITS-PCR-restriction fragment length polymorphism (ITS-PCR-RFLP) and randomly amplified polymorphic DNA (RAPD-PCR) further confirmed B. subtilis and B. amyloliquefaciens as the dominant Bacillus species associated with fermentation of iru, and revealed high strains genetic diversity, while multilocus sequence analysis (MLSA) data distinguished B. cereus from B. thuringiensis. This information is essential for selection of starter cultures with desirable functional attributes to guarantee product consistency and safety quality of traditional fermented foods. 相似文献
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The gram-positive Enterococci bacteria are generally used as a starter and probiotic cultures in foods. However, they have emerged as one of the leading causes of nosocomial infections worldwide, and this feature is aggravated by the development of antibiotic resistance. Accurate identification of Enterococci at the species level is an important task in food microbiology. In this study, 144 strains of Enterococcus species were isolated from traditional fermented vegetable condiment and West African soft cheese (wara) with the most predominant species being E. gallinarum (75%) followed by E. faecium (14.5%), E. faecalis (7.6%), and E. casselliflavus (2.8%). The strains isolated were characterized and identified using the polyphasic taxonomy approach. Phenotypically, 108 strains were characterized and identified to be E. gallinarum, 21 strains as E. faecium, 11 strains as E. faecalis, and 4 strains as E. casselliflavus. Thirty representative strains were also subjected to genomic characterization, and the result obtained with the phenotypic approach was confirmed. Therefore, the polyphasic taxonomy approach was successful in the accurate identification of the Enterococcus species isolated. 相似文献
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吴正格 《扬州大学烹饪学报》2008,25(3):37-41
以味为核心应是厨师在烹肴制馔中的首要职掌。厨师对味的审鉴主要是对鲜、活原料的质味(本味)的熟谙和把握以及对调味料的应用。作为调味料主干的酱调料,对菜肴的食效将起到至关重要的影响。以酱调料为主要调料可以制作出品种繁多的美味佳肴。 相似文献
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“色”是评价菜肴质量优劣的重要因素之一.在烹调过程中,只要添加合适的调味品,掌握调味品的调色机理,运用独特的调味品调色工艺手段,就可以使菜肴达到诱人食欲的“色”,满足消费者的心理味觉. 相似文献
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调味品行业发展成就显著,总体安全质量较好,但仍然存在诸多质量安全问题,需要进一步加强安全监管。近些年,我国调味品监管机制不断完善,监管力度不断加强,调味品监管总体成效较好,但在监管主体、监管机制、监管依据、监管措施、监管保障等方面存在不足,需要完善相应的对策,充分发挥监管效能,保障调味品行业健康发展。 相似文献