A Role for Human Renal Tubular Epithelial Cells in Direct Allo-Recognition by CD4+ T-Cells and the Effect of Ischemia-Reperfusion

Direct allorecognition is the earliest and most potent immune response against a kidney allograft. Currently, it is thought that passenger donor professional antigen-presenting cells (APCs) are responsible. Further, many studies support that graft ischemia-reperfusion injury increases the probability of acute rejection. We evaluated the possible role of primary human proximal renal tubular epithelial cells (RPTECs) in direct allorecognition by CD4+ T-cells and the effect of anoxia-reoxygenation. In cell culture, we detected that RPTECs express all the required molecules for CD4+ T-cell activation (HLA-DR, CD80, and ICAM-1). Anoxia-reoxygenation decreased HLA-DR and CD80 but increased ICAM-1. Following this, RPTECs were co-cultured with alloreactive CD4+ T-cells. In T-cells, zeta chain phosphorylation and c-Myc increased, indicating activation of T-cell receptor and co-stimulation signal transduction pathways, respectively. T-cell proliferation assessed with bromodeoxyuridine assay and with the marker Ki-67 increased. Previous culture of RPTECs under anoxia raised all the above parameters in T-cells. FOXP3 remained unaffected in all cases, signifying that proliferating T-cells were not differentiated towards a regulatory phenotype. Our results support that direct allorecognition may be mediated by RPTECs even in the absence of donor-derived professional APCs. Also, ischemia-reperfusion injury of the graft may enhance the above capacity of RPTECs, increasing the possibility of acute rejection.     

Electrospun microporous gelatin–polycaprolactone blend tubular scaffold as a potential vascular biomaterial

The work reported involved the fabrication of an electrospun tubular conduit of a gelatin and polycaprolactone (PCL) blend as an adventitia‐equivalent construct. Gelatin was included as the matrix for increased biocompatibility with the addition of PCL for durability. This is contrary to most of the literature available for biomaterials based on blends of gelatin and PCL where PCL is the major matrix. The work includes the assiduous selection of key electrospinning parameters to obtain smooth bead‐free fibres with a narrow distribution of pore size and fibre diameter. Few reports elucidate the optimization of all electrospinning parameters to fabricate tubular conduits with a focus on obtaining homogeneous pores and fibres. This stepwise investigation would be unique for the fabrication of gelatin–PCL electrospun tubular constructs. The fabricated microfibrous gelatin–PCL constructs had pores of size ca 50–100 μm reportedly conducive for cell infiltration. The measured value of surface roughness of 57.99 ± 17.4 nm is reported to be favourable for protein adhesion and cell adhesion. The elastic modulus was observed to be similar to that of the tunica adventitia of the native artery. Preliminary in vitro and in vivo biocompatibility tests suggest safe applicability as a biomaterial. Minimal cytotoxicity was observed using MTT assay. Subcutaneous implantation of the scaffold demonstrated acute inflammation which decreased by day 15. The findings of this study could enable the fabrication of smooth bead‐free microfibrous gelatin–PCL tubular construct as viable biomaterial which can be included in a bilayer or a trilayer scaffold for vascular tissue engineering. © 2019 Society of Chemical Industry     

旱地西红柿种植技术

随着人民生活水平的提高,旱地西红柿种植面积逐年增大,但由于种植技术的缺乏,产量和质量均得不到提高。文章通过多年的研究,对西红柿从育苗、施肥、覆膜种植到管理进行了系统的阐述,有效提高山区人民旱地西红柿的种植水平,增加农民收入。     

强捻棉纱针织产品的性能研究

通过对强捻棉纱针织产品进行服用性能测试，得出强捻纱织物具有良好的服用性能。     

运用NOAA AVHRR和Landsat TM数据估算多年水稻种植面积

介绍了综合运用NOAA AVHRR和Landsat TM数据进行多年水稻种植面积监测的一种方法,以湖北省为例,首先运用Landsat TM数据计算了该省1992年的水稻种植面积;接着运用1992年和1994年的NOAA AVHRR数据分别计算这两年的水稻像元数,以这两年水稻像元数的变化来反映水稻种植面积的变化;最后运用线性模型,估算1994年的水稻种植面积。所得的1994年水稻种植面积与湖北省农调队资料相比精度为84.5%。运用同样的方法估算1995年该省的水稻种植面积,精度达90%以上。     

秸杆材料用作草皮栽培基质的研究

本文介绍了秸杆毡的成型工艺 ,通过植物所需的水、肥料和空气三种因素对秸杆毡的化学和物理性能进行了研究 ,该材料可被用作草皮栽培基质     

复杂结构高耸建筑物爆破拆除切口研究

针对壁体材料多样、结构复杂的高耸建筑物的爆破拆除,通过实验研究确定了爆破切口形式,运用力学理论推导了余留支撑体所对应的圆心角和爆破切口高度计算公式,并在工程实践中得到验证.对爆破拆除类似建筑物具有较大的实用价值.     

环锭细纱机减少成纱毛羽的对策

通过对环锭细纱机成纱毛羽的成因分析 ,提出减少成纱毛羽的措施。     

植筋施工工艺

以某网球馆改造工程为例 ,就植筋技术的概念及所用材料进行了介绍 ,详细阐述了其施工工艺流程及措施 ,指出植筋技术具有工艺简单、操作方便、造价相对较低等优点     

简析Ⅲ类PHC管桩的成因及加固补强处理方法

本文主要通过Ⅲ类PHC管桩的形成分析,经过各方论证采用了合适的处理方法,取得了较好的效果。