Temperature-responsive culture surfaces for insect cell sheets to fabricate a bioactuator

ABSTRACT

For the first time, we have fabricated insect-derived cell sheets by using temperature-responsive culture surfaces having a phase-transition temperature below 25°C. We prepared the temperature-responsive cell culture surfaces (tissue culture polystyrene, TCPS) by grafting a copolymeric gel consisting of hydrophobic N-tert-butylacrylamide (tBAAm) and N-isopropylacrylamide (IPAAm) units. First, to characterize the hydrophilic and hydrophobic properties of the copolymeric gel-grafted surfaces, static water contact angles of each surface were measured at various temperatures. By increasing the amount of tBAAm in the grafted copolymeric gel, the transition temperature of the gel was shifted to lower temperatures. At 25°C, the grafted copolymeric gel was dehydrated, and the insect-derived cells (AeAl2 cells) adhered on all the copolymeric gel-grafted surfaces. At 20°C, AeAl2 cells cannot adhere on the P(IPAAm-1.62tBAAm)-TCPS surface (the initial molar ratio of IPAAm and tBAAm (tBAAm?=?1.62 mol%)) better than on other surfaces (TCPS and tBAAm?=?4.88, 8.17 mol%). These two findings implied that the lower critical solution temperature of the copolymeric gel-grafted-TCPS existed from 20°C to 25°C. The laminin-coated P(IPAAm-1.62tBAAm)-TCPS surface showed temperature-dependent cell attachment and detachment properties, while AeAl2 cells were not detached from the extracellular matrix uncoated P(IPAAm-1.62tBAAm)-TCPS surface. AeAl2 cells and insect muscle cells were harvested as the respective sheets.     

Domain structure-based transfer learning for cross-domain word representation

Cross-domain word representation aims to learn high-quality semantic representations in an under-resourced domain by leveraging information in a resourceful domain. However, most existing methods mainly transfer the semantics of common words across domains, ignoring the semantic relations among domain-specific words. In this paper, we propose a domain structure-based transfer learning method to learn cross-domain representations by leveraging the relations among domain-specific words. To accomplish this, we first construct a semantic graph to capture the latent domain structure using domain-specific co-occurrence information. Then, in the domain adaptation process, beyond domain alignment, we employ Laplacian Eigenmaps to ensure the domain structure is consistently distributed in the learned embedding space. As such, the learned cross-domain word representations not only capture shared semantics across domains, but also maintain the latent domain structure. We performed extensive experiments on two tasks, namely sentiment analysis and query expansion. The experiment results show the effectiveness of our method for tasks in under-resourced domains.     

Economic Optimization of Distribution Leakage Control

E vry WATER DISTRIBUTION system has an economic optimum level of leakage. To maintain that optimum level of leakage there is a corresponding optimum level of leakage control activity.
Despite much work on this subject, particularly in recent years with publication of DoE Report No. 26¹, many undertakers remain unclear about their economic optimum leakage level and how much effort is required to maintain that level.
This paper describes development of an economic model which attempts to quantify optimum leakage through measurement of parameters which should be readily available or obtainable.
The model was originally developed specifically for routine sounding. However, extension of the basic principles allows development of a general optimization theory. The paper then shows how the theory can be applied to optimization of district metering     

生态研究文献关键词分析对风景园林学科的启示

关键词分析是文献计量的一种方法。它能快速、客观和直观地了解某个学术领域的研究热点和趋势。随着人类对自然环境的重视,近年来有关生态的研究逐渐从纯粹的郊野自然走向城市自然。搜集了2004-2013年10年间《景观与城市规划》(Landscape and Urban Planning)期刊刊载的有关风景园林学生态方向的文献,将文献中的关键词按研究对象、研究方法以及研究热点进行统计和分类,提取出高频关键词,并根据文献摘要内容,对有关风景园林学科的生态研究进展进行了综述,总结出近10年期刊中有关风景园林学科生态研究热点,为掌握风景园林学科的发展动态提供依据。     

创新、努力与适应——上海东方体育中心游泳馆建筑材料运用侧记

文章从建筑材料创新运用入手,介绍了作为第14届国际泳联世界游泳锦标赛主赛场之一的东方体育中心游泳馆的建筑材料表现策略,重点阐述了建筑形态的设计创意和由此带来的材料设计针对性,集中探讨了玻璃纤维混凝土板（GRC）的设计实践可能,为未来更多新型材料的使用提供了宝贵经验。     

Word2003使用中的几个问题及解决

介绍Word常见的一些问题及解决方法。     

Tuning in to psychological change: Linguistic markers of psychological traits and emotions over time in popular U.S. song lyrics.

American culture is filled with cultural products. Yet few studies have investigated how changes in cultural products correspond to changes in psychological traits and emotions. The current research fills this gap by testing the hypothesis that one cultural product—word use in popular song lyrics—changes over time in harmony with cultural changes in individualistic traits. Linguistic analyses of the most popular songs from 1980–2007 demonstrated changes in word use that mirror psychological change. Over time, use of words related to self-focus and antisocial behavior increased, whereas words related to other-focus, social interactions, and positive emotion decreased. These findings offer novel evidence regarding the need to investigate how changes in the tangible artifacts of the sociocultural environment can provide a window into understanding cultural changes in psychological processes. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

利用Word邮件合并功能打印毕业证的技术实现

本文从打印毕业证的实际应用出发,根据Word邮件合并功能和Excel函数功能,以解决问题的思路为顺序,论述了完成中专毕业证打印设计工作的方法和步骤。