首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   1015篇
  免费   150篇
  国内免费   38篇
电工技术   4篇
综合类   26篇
化学工业   272篇
金属工艺   25篇
机械仪表   33篇
建筑科学   38篇
轻工业   574篇
无线电   39篇
一般工业技术   64篇
冶金工业   52篇
原子能技术   2篇
自动化技术   74篇
  2023年   30篇
  2022年   46篇
  2021年   160篇
  2020年   98篇
  2019年   89篇
  2018年   63篇
  2017年   59篇
  2016年   52篇
  2015年   46篇
  2014年   84篇
  2013年   73篇
  2012年   62篇
  2011年   86篇
  2010年   53篇
  2009年   34篇
  2008年   28篇
  2007年   34篇
  2006年   39篇
  2005年   20篇
  2004年   11篇
  2003年   6篇
  2002年   14篇
  2001年   3篇
  1999年   2篇
  1998年   2篇
  1996年   3篇
  1994年   2篇
  1993年   2篇
  1981年   1篇
  1980年   1篇
排序方式: 共有1203条查询结果,搜索用时 15 毫秒
1.
2.
Neutrophils are primary effector cells of innate immunity and fight infection by phagocytosis and degranulation. Activated neutrophils also release neutrophil extracellular traps (NETs) in response to a variety of stimuli. These NETs are net-like complexes composed of cell-free DNA, histones and neutrophil granule proteins. Besides the evolutionarily conserved mechanism to capture and eliminate pathogens, NETs are also associated with pathophysiological processes of various diseases. Here, we elucidate the mechanisms of NET formation and their different implications in disease. We focused on autoinflammatory and cardiovascular disorders as the leading cause of death. Neutrophil extracellular traps are not only present in various cardiovascular diseases but play an essential role in atherosclerotic plaque formation, arterial and venous thrombosis, as well as in the development and progression of abdominal aortic aneurysms. Furthermore, NETosis can be considered as a source of autoantigens and maintains an inflammatory milieu promoting autoimmune diseases. Indeed, there is further need for research into the balance between NET induction, inhibition, and degradation in order to pharmacologically target NETs and their compounds without impairing the patient’s immune defense. This review may be of interest to both basic scientists and clinicians to stimulate translational research and innovative clinical approaches.  相似文献   
3.
Sialidase cleaves sialic acid residues from glycans such as glycoproteins and glycolipids. In the brain, desorption of the sialic acid by sialidase is essential for synaptic plasticity, learning and memory and synaptic transmission. BTP3-Neu5Ac has been developed for sensitive imaging of sialidase enzyme activity in mammalian tissues. Sialidase activity in the rat hippocampus detected with BTP3-Neu5Ac increases rapidly by neuronal depolarization. It is presumed that an increased sialidase activity in conjunction with neural excitation is involved in the formation of the neural circuit for memory. Since sialidase inhibits the exocytosis of the excitatory neurotransmitter glutamate, the increased sialidase activity by neural excitation might play a role in the negative feedback mechanism against the glutamate release. Mammalian tissues other than the brain have also been stained with BTP3-Neu5Ac. On the basis of information on the sialidase activity imaging in the pancreas, it was found that sialidase inhibitor can be used as an anti-diabetic drug that can avoid hypoglycemia, a serious side effect of insulin secretagogues. In this review, we discuss the role of sialidase in the brain as well as in the pancreas and skin, as revealed by using a sialidase activity imaging probe. We also present the detection of influenza virus with BTP3-Neu5Ac and modification of BTP3-Neu5Ac.  相似文献   
4.
Mosquito-borne Zika virus (ZIKV) became a real threat to human health due to the lack of vaccine and effective antiviral treatment. The virus has recently been responsible for a global outbreak leading to millions of infected cases. ZIKV complications were highlighted in adults with Guillain–Barré syndrome and in newborns with increasing numbers of congenital disorders ranging from mild developmental delays to fatal conditions. The ability of ZIKV to establish a long-term infection in diverse organs including the kidneys has been recently documented but the consequences of such a viral infection are still debated. Our study aimed to determine whether the efficiency of ZIKV growth in kidney cells relates to glucose concentration. Human kidney HK-2 cells were infected with different ZIKV strains in presence of normal and high glucose concentrations. Virological assays showed a decrease in viral replication without modifying entry steps (viral binding, internalization, fusion) under high glucose conditions. This decrease replication was associated with a lower virus progeny and increased cell viability when compared to ZIKV-infected HK-2 cells in normal glucose concentration. In conclusion, we showed for the first time that an elevated glucose level influences ZIKV replication level with an effect on kidney cell survival.  相似文献   
5.
The aim of our study was to analyze mitochondrial and endoplasmic reticulum (ER) gene expression profiles in subcutaneous (SAT) and epicardial (EAT) adipose tissue, skeletal muscle, and myocardium in patients with and without CAD undergoing elective cardiac surgery. Thirty-eight patients, 27 with (CAD group) and 11 without CAD (noCAD group), undergoing coronary artery bypass grafting and/or valvular surgery were included in the study. EAT, SAT, intercostal skeletal muscle, and right atrium tissue and blood samples were collected at the start and end of surgery; mRNA expression of selected mitochondrial and ER stress genes was assessed using qRT-PCR. The presence of CAD was associated with decreased mRNA expression of most of the investigated mitochondrial respiratory chain genes in EAT, while no such changes were seen in SAT or other tissues. In contrast, the expression of ER stress genes did not differ between the CAD and noCAD groups in almost any tissue. Cardiac surgery further augmented mitochondrial dysfunction in EAT. In our study, CAD was associated with decreased expression of mitochondrial, but not endoplasmic reticulum stress genes in EAT. These changes may contribute to the acceleration of coronary atherosclerosis.  相似文献   
6.
Parkinson’s disease (PD) is a neurodegenerative disorder that affects 1% of the population over the age of 60. Diabetes Mellitus (DM) is a metabolic disorder that affects approximately 25% of adults over the age of 60. Recent studies showed that DM increases the risk of developing PD. The link between DM and PD has been discussed in the literature in relation to different mechanisms including mitochondrial dysfunction, oxidative stress, and protein aggregation. In this paper, we review the common microRNA (miRNA) biomarkers of both diseases. miRNAs play an important role in cell differentiation, development, the regulation of the cell cycle, and apoptosis. They are also involved in the pathology of many diseases. miRNAs can mediate the insulin pathway and glucose absorption. miRNAs can also regulate PD-related genes. Therefore, exploring the common miRNA biomarkers of both PD and DM can shed a light on how these two diseases are correlated, and targeting miRNAs is a potential therapeutic opportunity for both diseases.  相似文献   
7.
为探究低剂量酒精摄入对不同性别2型糖尿病(T2DM)小鼠影响的差异性及可能机制,采用链脲佐菌素(STZ)处理高脂高糖饲料喂养的小鼠构建T2DM模型,以灌胃200 mg/(kg·d)二甲双胍的T2DM小鼠为阳性对照,连续灌胃低剂量酒精(0.033 mL/d)30 d后,测定不同性别小鼠的糖脂代谢相关指标和胰腺组织氧化应激水平,并观察胰腺组织中胰岛的损伤、测定胰岛素的表达情况。结果表明,连续30 d摄入低剂量酒精可加重T2DM雄性小鼠糖脂代谢紊乱及胰腺组织中胰岛损伤,降低胰岛素表达量,上调T2DM雄性小鼠胰腺组织氧化应激水平,但并未对T2DM雌性小鼠产生显著影响,推测胰腺组织氧化应激水平的差异性影响可能是低剂量酒精对不同性别T2DM小鼠产生差异影响的主要机制之一。  相似文献   
8.
Ⅱ型糖尿病(Type 2 diabetes mellitus,T2DM)已成为全世界严重的公共卫生问题之一。近年来,以全谷物食品与生酮食品为代表,通过改善脂肪、碳水化合物、蛋白等相关营养结构的饮食方式被报道具有控制血糖血脂、减轻体重的效果,并且可以减少T2DM患者药物的使用量。综述了全谷物食品、生酮食品与T2DM相关研究的起源、在控制T2DM发展中的作用及机制;梳理了全谷物、生酮相关食品的开发利用情况、存在问题及发展趋势,基于目前两类食品在开发上均存在不同问题,随着其参与治疗T2DM病程的不断发展,开发一系列营养均衡、色香味俱全的全谷物食品和生酮食品是非常有必要的。  相似文献   
9.
Diabetes mellitus is a major healthcare problem. It is not only characterized by hyperglycemia and chronic complications, but in longer lasting diabetes and a longer living population, it is also associated with accelerated arterial ageing, which importantly contributes to cardiovascular complications. The accelerated arterial ageing in patients with diabetes should be considered separately from arterial ageing in patients without diabetes. Basic and clinical research have allowed better insight into the mechanisms of arterial ageing. In a simplified mechanistic way, it could be considered that the three tightly connected cornerstone characteristics of arterial ageing in patients with diabetes are: phenotypic presentation as endothelial dysfunction and arterial stiffness, and the underlying basic ageing-facilitating mechanism represented as the impaired expression of genetic longevity pathways. Currently, specific drugs for preventing/treating arterial ageing are not available. Therefore, we aimed to review the capacity of available drugs, particularly antidiabetic drugs, to interfere with the arterial ageing process. In the near future, these characteristics could help to guide therapy in patients with diabetes. Overall, it appears that arterial ageing could become a new target in diabetes. The expanding knowledge regarding the capability of antidiabetic drugs and other available drugs to inhibit/delay arterial aging is therefore essential.  相似文献   
10.
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号