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排序方式: 共有1601条查询结果,搜索用时 15 毫秒
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Matteo Di Giosia Alice Soldà Markus Seeger Andrea Cantelli Fabio Arnesano Maria I. Nardella Vincenzo Mangini Francesco Valle Marco Montalti Francesco Zerbetto Stefania Rapino Matteo Calvaresi Vasilis Ntziachristos 《Advanced functional materials》2021,31(20):2101527
Fullerenes are candidates for theranostic applications because of their high photodynamic activity and intrinsic multimodal imaging contrast. However, fullerenes suffer from low solubility in aqueous media, poor biocompatibility, cell toxicity, and a tendency to aggregate. C70@lysozyme is introduced herein as a novel bioconjugate that is harmless to a cellular environment, yet is also photoactive and has excellent optical and optoacoustic contrast for tracking cellular uptake and intracellular localization. The formation, water-solubility, photoactivity, and unperturbed structure of C70@lysozyme are confirmed using UV-visible and 2D 1H, 15N NMR spectroscopy. The excellent imaging contrast of C70@lysozyme in optoacoustic and third harmonic generation microscopy is exploited to monitor its uptake in HeLa cells and lysosomal trafficking. Last, the photoactivity of C70@lysozyme and its ability to initiate cell death by means of singlet oxygen (1O2) production upon exposure to low levels of white light irradiation is demonstrated. This study introduces C70@lysozyme and other fullerene-protein conjugates as potential candidates for theranostic applications. 相似文献
3.
Colour remains one of the key factors in presenting an object and, consequently, has been widely applied in retrieval of images based on their visual contents. However, a colour appearance changes with the change of viewing surroundings, the phenomenon that has not been paid attention yet while performing colour‐based image retrieval. To comprehend this effect, in this article, a chromatic contrast model, CAMcc, is developed for the application of retrieval of colour intensive images, cementing the gap that most of existing colour models lack to fill by taking simultaneous colour contrast into account. Subsequently, the model is applied to the retrieval task on a collection of museum wallpapers of colour‐rich images. In comparison with current popular colour models including CIECAM02, HSI and RGB, with respect to both foreground and background colours, CAMcc appears to outperform the others with retrieved results being closer to query images. In addition, CAMcc focuses more on foreground colours, especially by maintaining the balance between both foreground and background colours, while the rest of existing models take on dominant colours that are perceived the most, usually background tones. Significantly, the contribution of the investigation lies in not only the improvement of the accuracy of colour‐based image retrieval but also the development of colour contrast model that warrants an important place in colour and computer vision theory, leading to deciphering the insight of this age‐old topic of chromatic contrast in colour science. © 2014 Wiley Periodicals, Inc. Col Res Appl, 40, 361–373, 2015 相似文献
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Research funding has been seen as one of the most important resource in the reward system of science. And usage of publications denotes an interesting perspective of user behavior in scientific communication. This study aims to address the relationship between funding and Usage Count, which is a new metrics item established on the platform of Web of Science. Full records of 300,010 articles published in 2013 were downloaded in October 2015, and divided into six disciplines, including information science library science, education educational research, economics, computer science, materials science, and chemistry. Seven indicators were proposed to measure the impact, including Funding rate, Citation per paper, Usage rate, Usage per paper, Citation difference, Usage difference, and Conversion rate. It concluded funding has impact on usage and citation, and funded papers attract more usage, but varying in different disciplines. Usage Count can be used in the extension of citation metrics but with limits. This study originally engages with usage metrics and detected that there is positive correlation between usage and funding. 相似文献
6.
Alice Maurer Prof. Dr. Florian P. Seebeck 《Chembiochem : a European journal of chemical biology》2020,21(20):2908-2911
Ergothioneine has emerged as a crucial cytoprotectant in the pathogenic lifestyle of Mycobacterium tuberculosis. Production of this antioxidant from primary metabolites may be regulated by phosphorylation of Thr213 in the active site of the methyltransferase EgtD. The structure of mycobacterial EgtD suggests that this post-translational modification would require a large-scale change in conformation to make the active-site residue accessible to a protein kinase. In this report, we show that, under in vitro conditions, EgtD is not a substrate of protein kinase PknD. 相似文献
7.
Yu. N. Denisyuk N. M. Ganzherli I. M. Maurer S. A. Pisarevskaya 《Technical Physics Letters》1997,23(4):279-280
A thick-layer light-sensitive material consisting of glycerin-containing bichromated gelatin has been prepared and used to
record real-time volume holograms with 0.44 μm helium-cadmium laser radiation. The thickness of the layers is 400–600 μm.
The holographic sensitivity of the material is 1 J/cm2. The highest diffraction efficiency of holograms recorded using a symmetric system with parallel beams is 32%.
Pis’ma Zh. Tekh. Fiz. 23, 62–65 (April 12, 1997) 相似文献
8.
We consider the switchbox routing problem of two-terminal nets in the case when all thek nets lie on two adjacent sides of the rectangle. Our routing model is the standard two-layer model. We develop an optimal algorithm that routes all the nets whenever a routing exists. The routing obtained uses the fewest possible number of vias. A more general version of this problem (adjacent staircase) is also optimally solved.This research was supported in part by NSA Contract No. MDA-904-85H-0015, NSF Grant No. DCR-86-00378, and by NSF Engineering Research Centers Program NSFD CDR 88003012. 相似文献
9.
A new diffuser capable of scattering light in one direction (one-dimensional diffuser) has been developed and experimentally
tested. The diffuser is formed in a thick layer of dichromated gelatin. The scattering indicatrices of the diffuser measured
in two mutually perpendicular directions are presented. It is demonstrated that the indicatrix width in one direction is significantly
greater than that in the perpendicular direc-tion. However, no zero-order diffraction has been observed. The proposed one-dimensional
diffuser exhibits close scattering indicatrices for reading at a wavelength of 0.44 and 0.63 μm. 相似文献
10.
Prof. Dr.-Ing. A. M. Miri Dipl.-Ing. F. Schunn Dipl.-Ing. C. Sihler Dr. W. W. Maurer Dr. A. Ulbricht 《Electrical Engineering (Archiv fur Elektrotechnik)》1995,78(4):223-228
Übersicht Um die Betriebssicherheit großer supraleitender Magnetsysteme für die Kernfusionsforschung zu gewährleisten, muß die im Magnetsystem gespeicherte Energie schnell entladen werden können. Durch die veränderlichen großvolumigen Magnetfelder werden in benachbarten leitfähigen Strukturen durch Induktion hohe Wirbelströme hervorgerufen. Durch sie treten dort elektromagnetische Kräfte. auf, die einzelne Komponenten mechanisch überlasten können. Zur Berechnung dieser Kräfte wurde eine einfache numerischanalytische Methode entwickelt, die im folgenden vorgestellt und mit Finite-Elemente-Verfahren verglichen wird.
Calculation of eddy current densities and forces around large superconducting coils for nuclear fusion
Contents The reliable operation of the superconducting magnet systems used for the magnetic confinement in nuclear fusion research requires the ability of a fast discharge of the stored magnetic energy. The corresponding magnetic field variation gives rise to high eddy currents by inductive coupling with surrounding conductive components. The eddy current forces can lead to a mechanical overload of some of these components. A simple numerical-analytical method was developed in order to calculate these forces. This method is presented and compared with the Finite Element Method.相似文献