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1.
Giulia Andreani Enea Ferlizza Riccardo Cabbri Micaela Fabbri Elisa Bellei Gloria Isani 《International journal of molecular sciences》2021,22(1)
The recent introduction of the Asian yellow-legged hornet, Vespa velutina, into Europe has raised concern regarding the threat to honeybees and the competition with the European hornet, Vespa crabro. The aim of this study was to investigated essential (Mg, Fe, Zn, Cu) and non-essential (Cd and Pb) elements in these two species. Element concentrations were determined in the whole body and separately in the head, thorax and abdomen using atomic absorption spectrometry (AAS). The changes in essential element concentration and speciation during metamorphosis were also studied using size exclusion chromatography followed by AAS and proteomic analysis. In both species, the essential elements were more concentrated in the abdomen due to the presence of fat bodies. Magnesium, Fe and Zn concentrations were significantly higher in V. crabro than in V. velutina and could have been related to the higher aerobic energy demand of the former species required to sustain foraging flight. Low concentrations of Cd and Pb were indicative of low environmental exposure. The concentration and speciation of essential elements, particularly Fe, varied among the developmental stages, indicating a modification of ligand preferences during metamorphosis. Overall, the results in the present study provide a better understanding of the hornet metal metabolism and a foundation for additional studies. 相似文献
2.
A Bonci P Grillner A Siniscalchi NB Mercuri G Bernardi 《Canadian Metallurgical Quarterly》1997,9(11):2359-2369
Intracellular and whole-cell patch-clamp recordings were used to evaluate the actions of different metabotropic glutamate receptor (mGluR) agonists on the synaptic inputs evoked on principal cells of the rat mesencephalon. Bath application of the group III mGluR agonists L-2-amino-4-phosphonobutyric acid (L-AP4) and L-serine-O-phosphonobutanoate (L-SOP) did not change the holding current of the cells held at resting potential (-60 mV) but produced a dose-dependent inhibition of the amplitude of the excitatory and inhibitory events. L-AP4 and L-SOP were more effective at inhibiting the excitatory postsynaptic currents (EPSCs) than the GABA(A) and GABA(B) inhibitory postsynaptic currents (IPSCs). The suppressing effects of L-AP4 and L-SOP were antagonized by (S)-2-amino-2-methyl-4-phosphonobutanoic acid (MAP-4) but not by +/- -alpha-methyl-4-carboxyphenylglycine (MCPG). Moreover, the group II agonist (2S,1'S,2'S)-(carboxycyclopropyl)glycine (L-CCG1) and the group I agonist (RS)-3,5-dihydrophenylglycine (3,5-DHPG) depressed in a dose-related manner the EPSC, the GABA(A) IPSC and the GABA(B) IPSC. The suppressing effect of the two mGluRs agonists was partially antagonized by MCPG but not by MAP-4. In addition, both L-CCG1 and 3,5-DHPG caused an inward shift of the holding current. To characterize the site of action of the metabotropic receptor agonists, experiments were performed to examine the amplitude and ratio of EPSC and GABA(A) IPSC pairs. The increase of the s2/s1 ratio caused by the agonists suggests that the location of the inhibitory mGluRs was presynaptic. These results indicate that the activation of presynaptic mGluRs controls the release of excitatory and inhibitory transmitters on presumed dopaminergic cells within the ventral mesencephalon. 相似文献
3.
L. Bonci M. Gattobigio G. Iannaccone M. Macucci 《Journal of Computational Electronics》2002,1(1-2):49-53
We present a Monte Carlo simulation of two implementations of Quantum Cellular Automaton (QCA) circuits: one based on simple ground state relaxation and the other on the clocked cell scheme that has recently been proposed by Tóth and Lent. We focus on the time-dependent behavior of two basic circuits, a binary wire and a majority voting gate, and assess their maximum operating speed and temperature requirements for different sets of fabrication parameters. 相似文献
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A technique for the exact solution of the 3D Schrödinger equation in a structure with the effective mass varying in the direction transverse with respect to that of electron propagation is presented. Such a technique is applied to the study of the tunneling conductance between two stacked quantum wires as a function of the voltage applied to a top gate, whose action on the confinement potential is evaluated by means of a 3D Poisson solver. Results are then compared with those for an approximate model that allows separating the variables of the Schrödinger equation and with those for a simplified treatment of the gate action. 相似文献
6.
Roberto Bagnara Roberta Gori Patricia M. Hill Enea Zaffanella 《Information and Computation》2004,193(2):84-116
Logic languages based on the theory of rational, possibly infinite, trees have much appeal in that rational trees allow for faster unification (due to the safe omission of the occurs-check) and increased expressivity (cyclic terms can provide very efficient representations of grammars and other useful objects). Unfortunately, the use of infinite rational trees has problems. For instance, many of the built-in and library predicates are ill-defined for such trees and need to be supplemented by run-time checks whose cost may be significant. Moreover, some widely used program analysis and manipulation techniques are correct only for those parts of programs working over finite trees. It is thus important to obtain, automatically, a knowledge of the program variables (the finite variables) that, at the program points of interest, will always be bound to finite terms. For these reasons, we propose here a new data-flow analysis, based on abstract interpretation, that captures such information. We present a parametric domain where a simple component for recording finite variables is coupled, in the style of the open product construction of Cortesi et al., with a generic domain (the parameter of the construction) providing sharing information. The sharing domain is abstractly specified so as to guarantee the correctness of the combined domain and the generality of the approach. This finite-tree analysis domain is further enhanced by coupling it with a domain of Boolean functions, called finite-tree dependencies, that precisely captures how the finiteness of some variables influences the finiteness of other variables. We also summarize our experimental results showing how finite-tree analysis, enhanced with finite-tree dependencies, is a practical means of obtaining precise finiteness information. 相似文献
7.
The Impact of Nonalcoholic Fatty Liver Disease on Renal Function in Children with Overweight/Obesity
Lucia Pacifico Enea Bonci Gian Marco Andreoli Michele Di Martino Alessia Gallozzi Ester De Luca Claudio Chiesa 《International journal of molecular sciences》2016,17(8)
The association between nonalcoholic fatty liver disease (NAFLD) and chronic kidney disease has attracted interest and attention over recent years. However, no data are available in children. We determined whether children with NAFLD show signs of renal functional alterations, as determined by estimated glomerular filtration rate (eGFR) and urinary albumin excretion. We studied 596 children with overweight/obesity, 268 with NAFLD (hepatic fat fraction ≥5% on magnetic resonance imaging) and 328 without NAFLD, and 130 healthy normal-weight controls. Decreased GFR was defined as eGFR < 90 mL/min/1.73 m2. Abnormal albuminuria was defined as urinary excretion of ≥30 mg/24 h of albumin. A greater prevalence of eGFR < 90 mL/min/1.73 m2 was observed in patients with NAFLD compared to those without liver involvement and healthy subjects (17.5% vs. 6.7% vs. 0.77%; p < 0.0001). The proportion of children with abnormal albuminuria was also higher in the NAFLD group compared to those without NAFLD, and controls (9.3% vs. 4.0% vs. 0; p < 0.0001). Multivariate logistic regression analysis revealed that NAFLD was associated with decreased eGFR and/or microalbuminuria (odds ratio, 2.54 (confidence interval, 1.16–5.57); p < 0.05) independently of anthropometric and clinical variables. Children with NAFLD are at risk for early renal dysfunction. Recognition of this abnormality in the young may help to prevent the ongoing development of the disease. 相似文献
8.
The facility layout problem involves the optimal location of manufacturing facilities into a workshop. The classical approach to the layout design is carried out in two separate steps: the first step is the construction of the block layout, i.e. the location of the departments into the workshop, and the second step is the design of the material handling system. The separate optimization of these two aspects of the problem leads to solutions that can be far from the total optimum. In this paper, an integrated approach to the facilities and material handling system design is proposed. Referring to a physical model, named the bay structure , and to a unidirectional AGV system, a genetic approach is proposed to individuate the locations of the departments, the positions of the pickup/delivery stations and the direction of the flow-path. The minimization of material handling cost is adopted as optimality criterion. 相似文献
9.
The use of abstraction in the context of abstract data types, is investigated. Properties to be checked are formulas in a first order logic under Kleene's 3-valued interpretation. Abstractions are defined as pairs consisting of a congruence and a predicate interpretation. Three types of abstractions are considered,∀∀, ∀∃ and ∃0,1∀, and for each of them corresponding property preservation results are established. An abstraction refinement property is also obtained. It shows how one can pass from an existing abstraction to a (less) finer one. Finally, equationally specified abstractions in the context of equationally specified abstract data types are discussed and exemplified.On leave from the Department of Computer Science, “Al. I. Cuza” University, Iaşi 740083, RomaniaThe research reported in this paper was partially supported by the program ECO-NET 08112WJ/2004-2005 and by the National University Research Council of Romania, grants CNCSIS 632(28)/2004 and CNCSIS 632(50)/2005. 相似文献
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