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排序方式: 共有48条查询结果,搜索用时 15 毫秒
1.
Giulia Andreani Enea Ferlizza Riccardo Cabbri Micaela Fabbri Elisa Bellei Gloria Isani 《International journal of molecular sciences》2021,22(1)
The recent introduction of the Asian yellow-legged hornet, Vespa velutina, into Europe has raised concern regarding the threat to honeybees and the competition with the European hornet, Vespa crabro. The aim of this study was to investigated essential (Mg, Fe, Zn, Cu) and non-essential (Cd and Pb) elements in these two species. Element concentrations were determined in the whole body and separately in the head, thorax and abdomen using atomic absorption spectrometry (AAS). The changes in essential element concentration and speciation during metamorphosis were also studied using size exclusion chromatography followed by AAS and proteomic analysis. In both species, the essential elements were more concentrated in the abdomen due to the presence of fat bodies. Magnesium, Fe and Zn concentrations were significantly higher in V. crabro than in V. velutina and could have been related to the higher aerobic energy demand of the former species required to sustain foraging flight. Low concentrations of Cd and Pb were indicative of low environmental exposure. The concentration and speciation of essential elements, particularly Fe, varied among the developmental stages, indicating a modification of ligand preferences during metamorphosis. Overall, the results in the present study provide a better understanding of the hornet metal metabolism and a foundation for additional studies. 相似文献
2.
Natasha Luckovitch Enea Pagliano 《International Journal of Food Science & Technology》2020,55(3):1110-1118
A novel method for the determination of nitrite and nitrate in meat products is presented. The samples were ground and extracted in hot water with the presence of and internal standards. The solution was buffered with sodium bicarbonate and reacted with triethyloxonium tetrafluoroborate to convert nitrite and nitrate into EtNO2 and EtONO2. Such derivatives could be detected by headspace GC/MS in positive chemical ionisation mode with 0.05 µg g−1 and 1.0 µg g−1 LOD. The method was used for and quantitation in the 0.5-300 and 2.5-300 µg g−1 ranges. The method was applied for the analysis of fifteen meat products. Despite minimal sample preparation, the headspace sampling ensured a clean chromatography for over 135 analyses (throughput ten samples per hour). The proposed method offers selective GC/MS detection combined with high-precision isotope dilution calibration, it is suitable for metrological applications and can support regulations on meat safety (European Commission, 2011). 相似文献
3.
The Impact of Nonalcoholic Fatty Liver Disease on Renal Function in Children with Overweight/Obesity
Lucia Pacifico Enea Bonci Gian Marco Andreoli Michele Di Martino Alessia Gallozzi Ester De Luca Claudio Chiesa 《International journal of molecular sciences》2016,17(8)
The association between nonalcoholic fatty liver disease (NAFLD) and chronic kidney disease has attracted interest and attention over recent years. However, no data are available in children. We determined whether children with NAFLD show signs of renal functional alterations, as determined by estimated glomerular filtration rate (eGFR) and urinary albumin excretion. We studied 596 children with overweight/obesity, 268 with NAFLD (hepatic fat fraction ≥5% on magnetic resonance imaging) and 328 without NAFLD, and 130 healthy normal-weight controls. Decreased GFR was defined as eGFR < 90 mL/min/1.73 m2. Abnormal albuminuria was defined as urinary excretion of ≥30 mg/24 h of albumin. A greater prevalence of eGFR < 90 mL/min/1.73 m2 was observed in patients with NAFLD compared to those without liver involvement and healthy subjects (17.5% vs. 6.7% vs. 0.77%; p < 0.0001). The proportion of children with abnormal albuminuria was also higher in the NAFLD group compared to those without NAFLD, and controls (9.3% vs. 4.0% vs. 0; p < 0.0001). Multivariate logistic regression analysis revealed that NAFLD was associated with decreased eGFR and/or microalbuminuria (odds ratio, 2.54 (confidence interval, 1.16–5.57); p < 0.05) independently of anthropometric and clinical variables. Children with NAFLD are at risk for early renal dysfunction. Recognition of this abnormality in the young may help to prevent the ongoing development of the disease. 相似文献
4.
The facility layout problem involves the optimal location of manufacturing facilities into a workshop. The classical approach to the layout design is carried out in two separate steps: the first step is the construction of the block layout, i.e. the location of the departments into the workshop, and the second step is the design of the material handling system. The separate optimization of these two aspects of the problem leads to solutions that can be far from the total optimum. In this paper, an integrated approach to the facilities and material handling system design is proposed. Referring to a physical model, named the bay structure , and to a unidirectional AGV system, a genetic approach is proposed to individuate the locations of the departments, the positions of the pickup/delivery stations and the direction of the flow-path. The minimization of material handling cost is adopted as optimality criterion. 相似文献
5.
6.
The adsorption of 2,2′-bipyridine and its photoactive complexes with RuII and RhIII was studied on binary platinum—gold electrodes chosen as models of the bifunctional catalysts for water photo-decomposition. The adsorption isotherms show that the geometrical arrangement of the adsorbed species, and their interaction with the electrode surface depend on their structure and on the nature of the metallic substrate and the superficial composition of the catalyst. The interpretation of the role played by the relay molecules in the electrocatalytic photodecomposition of water allows a discussion of the preparation and the behaviour of Pt—Au binary catalysts used for the production of photolytic hydrogen. 相似文献
7.
Tanner P Baumann P Enea R Onaca O Palivan C Meier W 《Accounts of chemical research》2011,44(10):1039-1049
One strategy in modern medicine is the development of new platforms that combine multifunctional compounds with stable, safe carriers in patient-oriented therapeutic strategies. The simultaneous detection and treatment of pathological events through interactions manipulated at the molecular level offer treatment strategies that can decrease side effects resulting from conventional therapeutic approaches. Several types of nanocarriers have been proposed for biomedical purposes, including inorganic nanoparticles, lipid aggregates, including liposomes, and synthetic polymeric systems, such as vesicles, micelles, or nanotubes. Polymeric vesicles--structures similar to lipid vesicles but created using synthetic block copolymers--represent an excellent candidate for new nanocarriers for medical applications. These structures are more stable than liposomes but retain their low immunogenicity. Significant efforts have been made to improve the size, membrane flexibility, and permeability of polymeric vesicles and to enhance their target specificity. The optimization of these properties will allow researchers to design smart compartments that can co-encapsulate sensitive molecules, such as RNA, enzymes, and proteins, and their membranes allow insertion of membrane proteins rather than simply serving as passive carriers. In this Account, we illustrate the advances that are shifting these molecular systems from simple polymeric carriers to smart-complex protein-polymer assemblies, such as nanoreactors or synthetic organelles. Polymeric vesicles generated by the self-assembly of amphiphilic copolymers (polymersomes) offer the advantage of simultaneous encapsulation of hydrophilic compounds in their aqueous cavities and the insertion of fragile, hydrophobic compounds in their membranes. This strategy has permitted us and others to design and develop new systems such as nanoreactors and artificial organelles in which active compounds are simultaneously protected and allowed to act in situ. In recent years, we have created a variety of multifunctional, proteinpolymersomes combinations for biomedical applications. The insertion of membrane proteins or biopores into the polymer membrane supported the activity of co-encapsulated enzymes that act in tandem inside the cavity or of combinations of drugs and imaging agents. Surface functionalization of these nanocarriers permitted specific targeting of the desired biological compartments. Polymeric vesicles alone are relatively easy to prepare and functionalize. Those features, along with their stability and multifunctionality, promote their use in the development of new theranostic strategies. The combination of polymer vesicles and biological entities will serve as tools to improve the observation and treatment of pathological events and the overall condition of the patient. 相似文献
8.
The use of abstraction in the context of abstract data types, is investigated. Properties to be checked are formulas in a first order logic under Kleene's 3-valued interpretation. Abstractions are defined as pairs consisting of a congruence and a predicate interpretation. Three types of abstractions are considered,∀∀, ∀∃ and ∃0,1∀, and for each of them corresponding property preservation results are established. An abstraction refinement property is also obtained. It shows how one can pass from an existing abstraction to a (less) finer one. Finally, equationally specified abstractions in the context of equationally specified abstract data types are discussed and exemplified.On leave from the Department of Computer Science, “Al. I. Cuza” University, Iaşi 740083, RomaniaThe research reported in this paper was partially supported by the program ECO-NET 08112WJ/2004-2005 and by the National University Research Council of Romania, grants CNCSIS 632(28)/2004 and CNCSIS 632(50)/2005. 相似文献
9.
Roberto Bagnara Roberta Gori Patricia M. Hill Enea Zaffanella 《Information and Computation》2004,193(2):84-116
Logic languages based on the theory of rational, possibly infinite, trees have much appeal in that rational trees allow for faster unification (due to the safe omission of the occurs-check) and increased expressivity (cyclic terms can provide very efficient representations of grammars and other useful objects). Unfortunately, the use of infinite rational trees has problems. For instance, many of the built-in and library predicates are ill-defined for such trees and need to be supplemented by run-time checks whose cost may be significant. Moreover, some widely used program analysis and manipulation techniques are correct only for those parts of programs working over finite trees. It is thus important to obtain, automatically, a knowledge of the program variables (the finite variables) that, at the program points of interest, will always be bound to finite terms. For these reasons, we propose here a new data-flow analysis, based on abstract interpretation, that captures such information. We present a parametric domain where a simple component for recording finite variables is coupled, in the style of the open product construction of Cortesi et al., with a generic domain (the parameter of the construction) providing sharing information. The sharing domain is abstractly specified so as to guarantee the correctness of the combined domain and the generality of the approach. This finite-tree analysis domain is further enhanced by coupling it with a domain of Boolean functions, called finite-tree dependencies, that precisely captures how the finiteness of some variables influences the finiteness of other variables. We also summarize our experimental results showing how finite-tree analysis, enhanced with finite-tree dependencies, is a practical means of obtaining precise finiteness information. 相似文献
10.
It is well known that halons are ozone-depleting substances and their release into the atmosphere has contributed in the last
decades to the reduction in the ozone layer. In consequence to the Montreal Protocol, an international agreement designed
to gradually eliminate the use of such substances, fire protection industry researchers faced the challenge of finding effective
solutions to replace halon extinguishers. This research aims to provide a method to support the decision maker in the selection
of the most suitable extinguisher substance for a specific application. According to such framework, a set of quantitative
and qualitative criteria has been established in the decision-making problem. Such criteria have been properly regrouped in
clusters in order to better evaluate their relative importance by means of AHP method and the criteria have been used for
scoring the alternatives. The choice of an extinguisher substance rather than another has been subsequently carried out by
means of a fuzzy TOPSIS approach, which reflects the vagueness of qualitative criteria. 相似文献