Combinations of Piperine with Hydroxypropyl-β-Cyclodextrin as a Multifunctional System

Piperine is an alkaloid that has extensive pharmacological activity and impacts other active substances bioavailability due to inhibition of CYP450 enzymes, stimulation of amino acid transporters and P-glycoprotein inhibition. Low solubility and the associated low bioavailability of piperine limit its potential. The combination of piperine with 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) causes a significant increase in its solubility and, consequently, an increase in permeability through gastrointestinal tract membranes and the blood–brain barrier. X-ray powder diffraction (XRPD), differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR) were used to characterize interactions between piperine and HP-β-CD. The observed physicochemical changes should be combined with the process of piperine and CD system formation. Importantly, with an increase in solubility and permeability of piperine as a result of interaction with CD, it was proven to maintain its biological activity concerning the antioxidant potential (2,2-diphenyl-1-picryl-hydrazyl-hydrate assay), inhibition of enzymes essential for the inflammatory process and for neurodegenerative changes (hyaluronidase, acetylcholinesterase, butyrylcholinesterase).     

Metabolic Profiling of VOCs Emitted by Bacteria Isolated from Pressure Ulcers and Treated with Different Concentrations of Bio-AgNPs

Considering the advent of antibiotic resistance, the study of bacterial metabolic behavior stimulated by novel antimicrobial agents becomes a relevant tool to elucidate involved adaptive pathways. Profiling of volatile metabolites was performed to monitor alterations of bacterial metabolism induced by biosynthesized silver nanoparticles (bio-AgNPs). Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae and Proteus mirabilis were isolated from pressure ulcers, and their cultures were prepared in the presence/absence of bio-AgNPs at 12.5, 25 and 50 µg mL⁻¹. Headspace solid phase microextraction associated to gas chromatography–mass spectrometry was the employed analytical platform. At the lower concentration level, the agent promoted positive modulation of products of fermentation routes and bioactive volatiles, indicating an attempt of bacteria to adapt to an ongoing suppression of cellular respiration. Augmented response of aldehydes and other possible products of lipid oxidative cleavage was noticed for increasing levels of bio-AgNPs. The greatest concentration of agent caused a reduction of 44 to 80% in the variety of compounds found in the control samples. Pathway analysis indicated overall inhibition of amino acids and fatty acids routes. The present assessment may provide a deeper understanding of molecular mechanisms of bio-AgNPs and how the metabolic response of bacteria is untangled.     

Excellent destabilization effect and ideal desorption temperature in the hydride Mg2FeH6 substituted with yttrium; a first principles study

We investigated some properties of the hydride Mg₂FeH₆ substituted with yttrium by a first principles calculation. Some experimental results showed that 4d transition metal, yttrium serves as a good catalyst for magnesium based hydrogen storage alloys, but there are a few theoretical studies about magnesium based hydrides substituted with it. Mg₂FeH₆ is regarded as a cheaper material than pure MgH₂, while it is crystalized into Fm3m structure (space group 225). Although it has high hydrogen storage capacity, many investigations have not been devoted to it due to its extremely high thermodynamic stability. The yttrium substituted Mg₂FeH₆ exhibits very low energy of formation, and its desorption temperature, 75 °C is very suitable for practical hydrogen storage applications. Our results showed that Mg₂FeH₆ is destabilized effectively by yttrium substitution and introducing vacancy defects has additive effect to the improvement of dehydrogenation performance.     

Fundamentals of modelling concurrency using discrete relational structures

We consider relational structures such that is a set and are two binary relations on . For a number of different classes of structures we show that any structure can be represented as the intersection of its maximal extensions. Such a property – called extension completeness – can be seen as a generalisation of Szpilrajn's theorem which states that each partial order is the intersection of its total order extensions. When can be interpreted as causality and as ‘weak’ causality we obtain a model of concurrent histories generalising that based on causal partial orders. Received 1 February 1994 / 6 May 1996     

Modeling of systems with overflow multi-rate traffic

The article proposes an analytical method for determining occupancy distribution and blocking probability in systems which are offered overflow traffic composed of multi-service traffic streams. The described analytical model enables determination of parameters of traffic overflowed from primary groups in hierarchically constructed telecommunication networks. The proposed method is based on an appropriate modification of the Kaufman-Roberts recursion for the full-availability group with multi-rate traffic and uses the modified Fredericks & Hayward’s approximation. Additionally, an approximate method for dimensioning systems with multi-service overflow traffic is also presented. The analytical results of the blocking probability and the results obtained in the dimensioning processes calculated using the presented methodology are compared with the data obtained from the system simulation process.     

Optimal predictor for a singular random process

An optimal predictor is developed for a singular random process generated by a known system driven by a white noise sequence. The properties of the predictor are compared with those of the optimal predictor for a nonsingular random process.     

Influence of the amount of ferromagnetic addition on the rheological properties of spinning solutions,the structure,the strength,and thermal properties of polyacrylonitrile fibres

The rheological properties of spinning solutions of polyacrylonitrile in dimethyl formamide (DMF) containing various amounts of a ferromagnetic nanoaddition were investigated. The porous structure, the strength and thermal properties of fibers obtained from these solutions were assessed, as well as the uniformity of the nanoaddition distribution on the fiber surface was estimated. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008     

Genotoxic effect of triarylmethane dye--acid green 16 after chronic ethanol consumption in mice

Genotoxic effect and hepatic microsomal monooxygenase activities were assessed in mice treated with Acid Green 16 (single i.p. injection at dose 75 mg/kg) superimposed on prolonged ethanol consumption (10% solution in drinking water for 2-4 months). Treatment of mice with Acid Green 16 led to an increased frequency of micronucleated erythrocytes in bone marrow. In animals pretreated with ethanol the frequency of micronucleated erythrocytes, produced by Acid Green 16, was significantly higher. The changes in frequency of micronucleated erythrocytes were accompanied by the enhanced activity of microsomal monooxygenases manifested by higher activity of 7-ethoxycoumarin o-deethylase (the level of cytochrome P-450 was not altered). The obtained results showed that ethanol tended to increase the genotoxic effect of Acid Green 16. However, the slight inductive effect of ethanol on microsomal monooxygenases did not provide clear evidence that the genotoxic effect of Acid Green 16 was associated with ethanol stimulation of the metabolic activation of the dye in the liver.     

Mode of deformation and the rate of energy storage during uniaxial tensile deformation of austenitic steel

The mechanism of slip and its consequence in the process of energy storage during uniaxial tension of austenitic steel was studied. The interpretation of the energy storage process in terms of the slip development and microscopic shear band formation is presented.     

Inverse Analysis of Axisymmetrical Compression of HSLA Steel

The objective of the present work is to further explore the problem of selection of the flow stress function which will give the best agreement with experiments for a wide range of the Zener‐Hollomon parameter. Analysis of various flow stress functions was performed, with particular emphasis on the Zerilli‐Armstrong model. Inverse analysis was successfully applied to identify the flow stress model for microalloyed steels deformed in ferrite, two‐phase and austenite regions. Inverse method is applied to interpret the results of the axisymmetrical compression tests performed for HSLA steel samples on a Gleeble 3800 and Split Hopkinson Pressure Bar. Sensitivity analysis is performed based on local and global methods. The objective of the sensitivity analysis in the present study is the evaluation to what extent the selected coefficients in the rheological model influence the result of simulations. In the considered tests this result is represented by two dependent variables, the load and the shape of the sample (barrelling). It is confirmed that the Zerilli‐Armstrong equations should be applied at very high strain rates. Physical meaning is an advantage of this model. Difficulties with identification are the main disadvantage.