Electrospun microporous gelatin–polycaprolactone blend tubular scaffold as a potential vascular biomaterial

The work reported involved the fabrication of an electrospun tubular conduit of a gelatin and polycaprolactone (PCL) blend as an adventitia‐equivalent construct. Gelatin was included as the matrix for increased biocompatibility with the addition of PCL for durability. This is contrary to most of the literature available for biomaterials based on blends of gelatin and PCL where PCL is the major matrix. The work includes the assiduous selection of key electrospinning parameters to obtain smooth bead‐free fibres with a narrow distribution of pore size and fibre diameter. Few reports elucidate the optimization of all electrospinning parameters to fabricate tubular conduits with a focus on obtaining homogeneous pores and fibres. This stepwise investigation would be unique for the fabrication of gelatin–PCL electrospun tubular constructs. The fabricated microfibrous gelatin–PCL constructs had pores of size ca 50–100 μm reportedly conducive for cell infiltration. The measured value of surface roughness of 57.99 ± 17.4 nm is reported to be favourable for protein adhesion and cell adhesion. The elastic modulus was observed to be similar to that of the tunica adventitia of the native artery. Preliminary in vitro and in vivo biocompatibility tests suggest safe applicability as a biomaterial. Minimal cytotoxicity was observed using MTT assay. Subcutaneous implantation of the scaffold demonstrated acute inflammation which decreased by day 15. The findings of this study could enable the fabrication of smooth bead‐free microfibrous gelatin–PCL tubular construct as viable biomaterial which can be included in a bilayer or a trilayer scaffold for vascular tissue engineering. © 2019 Society of Chemical Industry     

Batch cultures of Penicillium camembertii on glucose and amino acids—model for growth and ammonium production

BACKGROUND: Three groups of amino acids were previously characterized based on their ability to be assimilated as carbon source by Penicillium camembertii. To describe the diauxic growth recorded on glucose and amino acids from the second group, such as arginine, an unstructured model was previously developed, based on the sequential consumption of both carbon substrates, glucose, followed after its exhaustion, by arginine. The model was modified to describe also the behaviour recorded during growth on other amino acids. RESULTS: The growth model involved the carbon substrate consumption (Verlhust model) and the biomass on carbon substrate yield. Glucose was therefore considered during P. camembertii growth on nitrogen source amino acids (lysine—first group); and amino acid consumption was considered during growth on carbon source amino acids (glutamate—third group), with glucose being dissimilated only for energy supply. The excess nitrogen from amino acids was released as ammonium; the linking of this production to growth was found to increase with the ability of the amino acid to be assimilated as carbon source by P. camembertii. CONCLUSION: The various metabolic behaviours recorded during P. camembertii growth on amino acids, in the presence of a primary carbon source such as glucose, were proved to be satisfactorily described by the model, showing the robustness of the model. Copyright © 2007 Society of Chemical Industry     

Reducing model checking commitments for agent communication to model checking ARCTL and GCTL*

Social commitments have been extensively and effectively used to represent and model business contracts among autonomous agents having competing objectives in a variety of areas (e.g., modeling business processes and commitment-based protocols). However, the formal verification of social commitments and their fulfillment is still an active research topic. This paper presents CTLC⁺ that modifies CTLC, a temporal logic of commitments for agent communication that extends computation tree logic (CTL) logic to allow reasoning about communicating commitments and their fulfillment. The verification technique is based on reducing the problem of model checking CTLC⁺ into the problem of model checking ARCTL (the combination of CTL with action formulae) and the problem of model checking GCTL^* (a generalized version of CTL^* with action formulae) in order to respectively use the extended NuSMV symbolic model checker and the CWB-NC automata-based model checker as a benchmark. We also prove that the reduction techniques are sound and the complexity of model checking CTLC⁺ for concurrent programs with respect to the size of the components of these programs and the length of the formula is PSPACE-complete. This matches the complexity of model checking CTL for concurrent programs as shown by Kupferman et al. We finally provide two case studies taken from business domain along with their respective implementations and experimental results to illustrate the effectiveness and efficiency of the proposed technique. The first one is about the NetBill protocol and the second one considers the Contract Net protocol.     

A formal method for synthesizing optimized protocol converters and its application to mobile data networks

As mobile information networks are expanding rapidly, we expect to integrate voice, paging, electronic mail and other wireless information services. Interworking units that perform protocol conversion at the boundaries of different networks will play an important role. In this paper, we propose an efficient algorithm for constructing optimized protocol converters to achieve interoperability between heterogeneous data networks. This algorithm first derives constraints from two given protocols, and apply the constraints to channel specifications, thus removing message sequences that do not contribute to system progress. Then, an optimized converter is generated from a given service specification, the two protocol specifications and the modified channel specifications. A reduction relation is used to compare the service specification and the constructed internetworking system in order to deal with the problem of nondeterministic services. Compared with related works, our method has two advantages: (1) it generates an optimized converter; (2) it can be applied to the case that the service specification is nondeterministic. The application of the method to mobile networks is given by an example. This revised version was published online in June 2006 with corrections to the Cover Date.     

Individual Influence of Climate Variability Indices on Annual Maximum Precipitation Across the Global Scale

Water Resources Management - Studying the influence of climate variability indices on extreme precipitation will help to understand the variability of extreme precipitation. However, the influence...     

Synthesis of MIL-101@g-C<Subscript>3</Subscript>N<Subscript>4</Subscript> nanocomposite for enhanced adsorption capacity towards CO<Subscript>2</Subscript>

MIL-101@g-C₃N₄ nanocomposite was prepared by solvothermal synthesis and used for CO₂ adsorption. The parent materials (MIL-101 and g-C₃N₄) and the MIL-101@g-C₃N₄ were characterized by X-ray diffraction, argon adsorption/desorption, Fourier transform infrared spectroscopy, thermal analysis (TG/DTA), transmission electronic microscopy, and Energy-dispersive X-ray spectroscopy. The results confirmed the formation of well-defined MIL-101@g-C₃N₄ with interesting surface area and pore volume. Furthermore, both MIL-101 and MIL-101@g-C₃N₄ were accomplished in carbon dioxide capture at different temperatures (280, 288, 273 and 298 K) at lower pressure. The adsorption isotherms show that the nanocomposite has a good CO₂ adsorption affinity compared to MIL-101. The best adsorption capacity is about 1.6 mmol g^?1 obtained for the nanocomposite material which is two times higher than that of MIL-101, indicating strong interactions between CO₂ and MIL-101@g-C₃N₄. This difference in efficacy is mainly due to the presence of the amine groups dispersed in the nanocomposite. Finally, we have developed a simple route for the preparation of an effective and new adsorbent for the removal of CO₂, which can be used as an excellent candidate for gas storage, catalysis, and adsorption.     

Tuning Physicochemical Properties of a Macroporous Polysaccharide-Based Scaffold for 3D Neuronal Culture

Central nervous system (CNS) lesions are a leading cause of death and disability worldwide. Three-dimensional neural cultures in biomaterials offer more physiologically relevant models for disease studies, toxicity screenings or in vivo transplantations. Herein, we describe the development and use of pullulan/dextran polysaccharide-based scaffolds for 3D neuronal culture. We first assessed scaffolding properties upon variation of the concentration (1%, 1.5%, 3% w/w) of the cross-linking agent, sodium trimetaphosphate (STMP). The lower STMP concentration (1%) allowed us to generate scaffolds with higher porosity (59.9 ± 4.6%), faster degradation rate (5.11 ± 0.14 mg/min) and lower elastic modulus (384 ± 26 Pa) compared with 3% STMP scaffolds (47 ± 2.1%, 1.39 ± 0.03 mg/min, 916 ± 44 Pa, respectively). Using primary cultures of embryonic neurons from PGK^Cre, Rosa26^tdTomato embryos, we observed that in 3D culture, embryonic neurons remained in aggregates within the scaffolds and did not attach, spread or differentiate. To enhance neuronal adhesion and neurite outgrowth, we then functionalized the 1% STMP scaffolds with laminin. We found that treatment of the scaffold with a 100 μg/mL solution of laminin, combined with a subsequent freeze-drying step, created a laminin mesh network that significantly enhanced embryonic neuron adhesion, neurite outgrowth and survival. Such scaffold therefore constitutes a promising neuron-compatible and biodegradable biomaterial.     

Role of the nature of reaction products in the differing behaviours of fine glass powders and coarse glass aggregates used in concrete

This paper deals with the incorporation of glass cullet in cement-based materials. The aim is to help understand the differing behaviours observed depending on the particle size of the glass: the use of fine powders usually improves the concrete properties due to pozzolanic reaction, while coarse aggregates are generally detrimental for concrete due to alkali-silica reaction. It is shown that the lack of swelling of gels resulting from the reaction of glass fines can be partly due to the nature of these gels. The comparative investigation carried out on reaction products resulting from glass grains of various sizes, in the presence of both portlandite Ca(OH)₂ and C₃S (tricalcium silicate: 3CaO · SiO₂), has shown that glass aggregates produce detrimental gels while glass fines produce gels that help to improve concrete properties.