(S)-Reutericyclin: Susceptibility Testing and In Vivo Effect on Murine Fecal Microbiome and Volatile Organic Compounds

We aimed to assess the in vitro antimicrobial activity and the in vivo effect on the murine fecal microbiome and volatile organic compound (VOC) profile of (S)-reutericyclin. The antimicrobial activity of (S)-reutericyclin was tested against Clostridium difficile, Listeria monocytogenes, Escherichia coli, Enterococcus faecium, Staphylococcus aureus, Staphylococcus (S.) epidermidis, Streptococcus agalactiae, Pseudomonas aeruginosa and Propionibacterium acnes. Reutericyclin or water were gavage fed to male BALBc mice for 7 weeks. Thereafter stool samples underwent 16S based microbiome analysis and VOC analysis by gas chromatography mass spectrometry (GC-MS). (S)-reutericyclin inhibited growth of S. epidermidis only. Oral (S)-reutericyclin treatment caused a trend towards reduced alpha diversity. Beta diversity was significantly influenced by reutericyclin. Linear discriminant analysis Effect Size (LEfSe) analysis showed an increase of Streptococcus and Muribaculum as well as a decrease of butyrate producing Ruminoclostridium, Roseburia and Eubacterium in the reutericyclin group. VOC analysis revealed significant increases of pentane and heptane and decreases of 2,3-butanedione and 2-heptanone in reutericyclin animals. The antimicrobial activity of (S)-reutericyclin differs from reports of (R)-reutericyclin with inhibitory effects on a multitude of Gram-positive bacteria reported in the literature. In vivo (S)-reutericyclin treatment led to a microbiome shift towards dysbiosis and distinct alterations of the fecal VOC profile.     

Single-mode AlGaAs-GaAs lasers using lateral confinement bynative-oxide layers

We report on results of wet oxidized narrow-stripe QW laser diodes operating in single lateral and longitudinal mode around an emission wavelength of 850 nm. Devices with an active width of 4-10 μm achieved output powers of up to 240 mW in continuous-wave (CW) operation at room temperature     

Improved output performance of high-power VCSELs

The intention of this paper is to report on state-of-the-art high-power vertical-cavity surface-emitting laser diodes (VCSELs), single devices as well as two-dimensional (2-D) arrays. Both approaches are studied in terms of electrooptical characteristics, beam performance, and scaling behavior. The maximum continuous wave (CW) output power at room temperature of large-area bottom-emitting devices with active diameters up to 320 μm is as high as 0.89 W, which is to our knowledge the highest value reported for a single device. Measurements under pulsed conditions show more than 10-W optical peak output power. Also, the CW performance of 2-D arrays has been increased from 0.56 W for 23 elements to 1.55 W for 19 elements due to significantly improved heat sinking. The extracted power densities spatially averaged over the area close to the honeycomb-like array arrangement raised from 0.33 kW/cm² to 1.25 kW/cm². Lifetime measurements have proven acceptable reliability for over 10000 h at a degradation rate of less than 1% per 1000 h. The emission wavelength of bottom-emitting devices is restricted to about 900 nm or higher due to fundamental absorption in the GaAs substrate. Windowing of the substrate has been studied to allow for shorter wavelength emission     

High performance selectively oxidized VCSELs and arrays forparallel high-speed optical interconnects

High-bandwidth single-mode selectively oxidized vertical-cavity surface-emitting laser (VCSEL) arrays operate at 980 nm or 850 nm emission wavelength for substrate or epitaxial side emission. Coplanar feeding lines and polyimide passivation are used to reduce electrical parasitics in top-emitting GaAs and bottom-emitting InGaAs VCSELs. To enhance fundamental single-mode emission for larger devices of reduced series resistance a surface relief transverse mode filter is employed. Fabricated VCSELs are applied in various interconnect schemes. InGaAs quantum-well based VCSELs at 935 nm emission wavelength are investigated for use in perfluorinated graded-index plastic-optical fiber (GI-POF) links. We obtain a 7 Gb/s pseudo random bit sequence (PRBS) nonreturn-to-zero (NRZ) data transmission over 80 m long 155 μm diameter GI-POF. We investigate data transmission over standard 1300 nm, 9 μm core diameter single-mode fiber with selectively oxidized single-mode GaAs and InGaAs VCSELs. We achieve biased 3 Gb/s and bias-free 1 Gb/s pseudo-random data transmission over 4.3 km at 830 nm emission wavelength where a simple fiber mode filter is used to suppress intermodal dispersion caused by the second order fiber mode. For the first time, we demonstrate 12.5 Gb/s data rate transmission of PRBS signals over 100 m graded-index multimode fiber or 1 km single-mode fiber using high performance single-mode GaAs VCSELs of 12.3 GHz modulation bandwidth emitting at λ=850 nm     

High-power single-mode selectively oxidized vertical-cavity surface-emitting lasers

Single-mode vertical-cavity top-surface-emitting lasers with maximum output power of 2.7 mW and side-mode suppression of 50 dB have been fabricated using solid source MBE for growth and selective oxidation to define the 3-/spl mu/m diameter active area. Threshold current is 290 /spl mu/A and a maximum wallplug efficiency of 27% is obtained at an output power of 1 mW.     

GaAs VCSEL's at λ=780 and 835 nm for short-distance 2.5-Gb/splastic optical fiber data links

Selectively oxidized GaAs vertical-cavity surface-emitting lasers for λ=780- and 835-nm emission wavelength and 120-μm-core diameter step index plastic optical fiber (POF) are investigated for short distance interconnects. 2.5-Gb/s pseudorandom data transmission over up to 2.5 m of plastic fiber is demonstrated with a bit-error rate (BER) of better than 10^-11. Furthermore, bias-free data transmission at 2.5 Gb/s over 1-m fiber length again at a BER of better than 10^-11 is reported     

High-power VCSEL arrays for emission in the watt regime at roomtemperature

Selectively oxidized InGaAs vertical-cavity surface emitting lasers (VCSELs) at an emission wavelength of λ=980 mm are investigated for high-power applications. Densely packed arrays consisting of 19 single devices with an active diameter of 50 μm emit 1.08 W of continuous-wave (CW) optical output power at room temperature. At 10°C, heat sink temperature the output power increases to 1.4 W, which corresponds to a chip size averaged power density of 1 kW·cm². Low divergence angle of less than 16° full-width at half-maximum (FWHM) and the circularly symmetric far-field pattern allow for simple focusing of the beam with power densities above 10 kW·cm²     

Bottom-emitting VCSEL's for high-CW optical output power

Bottom-emitting vertical-cavity surface-emitting InGaAs MQW lasers operating in the 980-nm wavelength regime have been designed for high continuous-wave optical output power. Devices of 200-μm active diameter and optimized performance reach 350-mW maximum output power when mounted on a heat sink. 50-μm-size lasers produce 100 mW at 25% electrical to optical power conversion efficiency. Thermal properties and size dependent basic characteristics are investigated in detail     

Efficient single-mode oxide-confined GaAs VCSEL's emitting in the850-nm wavelength regime

Single- and multimode vertical-cavity surface-emitting lasers (VCSELs) with three unstrained GaAs quantum wells (QWs) and emission wavelengths around 850 nm have been fabricated using molecular beam epitaxy (MBE) for crystal growth. Wet chemical etching and subsequent selective oxidation are applied for current confinement. The influence of oxide layer position on lateral index guiding is studied in detail in order to increase maximum single-mode output power. A device of 3-μm active diameter and reduced index guiding shows maximum single-mode output power of 2.25 mW with a side-mode suppression ratio (SMSR) of more than 30 dB for high-efficiency oxidized VCSELs     

Angiofil-mediated visualization of the vascular system by microcomputed tomography: a feasibility study

Visualization of the vascular systems of organs or of small animals is important for an assessment of basic physiological conditions, especially in studies that involve genetically manipulated mice. For a detailed morphological analysis of the vascular tree, it is necessary to demonstrate the system in its entirety. In this study, we present a new lipophilic contrast agent, Angiofil, for performing postmortem microangiography by using microcomputed tomography. The new contrast agent was tested in 10 wild-type mice. Imaging of the vascular system revealed vessels down to the caliber of capillaries, and the digital three-dimensional data obtained from the scans allowed for virtual cutting, amplification, and scaling without destroying the sample. By use of computer software, parameters such as vessel length and caliber could be quantified and remapped by color coding onto the surface of the vascular system. The liquid Angiofil is easy to handle and highly radio-opaque. Because of its lipophilic abilities, it is retained intravascularly, hence it facilitates virtual vessel segmentation, and yields an enduring signal which is advantageous during repetitive investigations, or if samples need to be transported from the site of preparation to the place of actual analysis, respectively. These characteristics make Angiofil a promising novel contrast agent; when combined with microcomputed tomography, it has the potential to turn into a powerful method for rapid vascular phenotyping.