重组人乳铁蛋白对子代大鼠免疫功能影响的一代繁殖毒性扩展试验

目的研究转基因牛乳腺生物反应器表达的重组人乳铁蛋白(rh LF)对子代大鼠免疫功能的影响。方法参照一代繁殖毒性扩展试验指南设计试验,将Wister大鼠分为对照组(酪蛋白)、重组人乳铁蛋白组,每组30只雌鼠、15只雄鼠;按照蛋白占比为20%的比重掺入饲料进行全食物喂养。雌/雄大鼠喂养相应饲料两周后进行交配,各组雌鼠在交配期、妊娠期和哺乳期继续给予相应饲料,子代大鼠从断乳到处死继续给予相应饲料。子代大鼠分别在PND0(出生后0天)、PND21(出生后21天)和PND56(出生后56天)进行免疫功能评价。结果重组人乳铁蛋白组与对照组比较,重组人乳铁蛋白组子代大鼠血常规、血生化和淋巴细胞分型结果未见明显改变;溶血空斑试验(PFC)、淋巴细胞增殖试验及自然杀伤细胞(NK细胞)活性试验指标结果均差异无统计学意义(P0.05)。结论在本研究的试验条件下,重组人乳铁蛋白对子代大鼠免疫功能未见不良影响。     

亲代大鼠转Bt基因水稻暴露对其子代部分早期生理和神经发育指标的影响

研究亲代大鼠转Bt基因水稻(TT51大米)暴露对其子代部分早期生理和神经发育指标的影响。方法 亲代大鼠随机分为3组,每组雄性15只,雌性30只,分别给予按60%比例掺入市售大米、明恢63大米和TT51大米的饲料,连续喂养70d后同组雄雌大鼠交配并产生子代,孕期和哺乳期各组母鼠继续给予相应受试大米的饲料。亲代大鼠每周称量和记录体重及食物消耗量,同时观察动物生长发育状况,观察和记录亲代雌鼠生殖指标。仔鼠出生后记录0、4、7、14及21d体重,并观察仔鼠部分生理指标发育情况,同时进行听觉惊愕等神经发育指标的测定。断乳后,对各试验组部分仔鼠取脑等主要脏器进行病理检查。结果 TT51大米组与明恢63大米组和市售大米组比较,亲代大鼠一般毒性及生殖指标间差异均无统计学意义;子代部分生理发育指标和神经发育指标差异均无统计学意义;各试验组仔鼠脏器病理检查均未见有意义的变化。结论 未见亲代大鼠转Bt基因水稻暴露对其子代早期部分生理和神经发育指标有不良影响。     

Observation of the Two-Dimensional Distribution of Impurity Line Emissions Using a Space-Resolved EUV Spectrometer in LHD

A space-resolved EUV spectrometer for measuring the one-dimensional distribution of impurity line emissions in Large Helical Device (LHD) has been upgraded to measure two- dimensional distributions of impurity line emissions with an extension of working wavelength range to 30～650 A. The two-dimensional measurement is performed by scanning the observation chord horizontally. A rectangular plasma region of 520 × 700 mm 2 in vertical and horizontal sizes can be observed during a single horizontal scan. The horizontal scan requires a time duration of 5 s at least. The spatial resolution is 10 mm in the vertical direction when a spatial-resolution slit of 0.2 mm in width is adopted. Although a spatial resolution in the toroidal direction is 75 mm, it is a function of CCD exposure time and horizontal scanning speed. Two-dimensional distribution of EUV line emissions from several impurities has been successfully observed for the first time from steady discharges in LHD. In this paper two-dimensional distributions of He II (303.78 A), C V (40.27 A), C VI (33.73 A) and Fe XX (132.85 A) located at different radial positions are presented with simple analysis on the magnetic field structure of LHD.     

Observation of Impurity Accumulation After Hydrogen Multi-Pellet Injection in Large Helical Device

Impurity accumulation is studied for neutral beam-heated discharges after hydrogen multi-pellet injection in Large Helical Device (LHD). Iron density profiles are derived from radial profiles of EUV line emissions of FeXV-XXIV with the help of the collisional-radiative model. A peaked density profile of Fe²³⁺ is simulated by using one-dimensional impurity transport code. The result indicates a large inward velocity of -6 m/s at the impurity accumulation phase. However, the discharge is not entirely affected by the impurity accumulation, since the concentration of iron impurity, estimated to be 3.3x10^-5 to the electron density, is considerably small. On the other hand, a flat profile is observed for the carbon density of C⁶⁺, which is derived from the Z_eff profile, indicating a small inward velocity of -1 m/s. These results suggest atomic number dependence in the impurity accumulation of LHD, which is similar to the tokamak result.     

转Bt基因大米暴露对雄性子代生殖系统影响的研究

目的 研究转Bt基因大米(TT51)暴露对Wistar大鼠雄性子代生殖系统发育的影响.方法 亲代雌雄大鼠分别连续给予市售大米、亲本大米和TT51大米70 d后,交配并产生子代,孕期和哺乳期各组雌性大鼠继续给予相应受试大米;子代雄性大鼠断乳后各组均给予普通饲料至70日龄,其间每周称量体重并记录食物消耗量和动物生长发育状况.雄性仔鼠至70日龄处死,进行血常规、血生化、血清性激素水平、生殖器官重量以及精子参数等指标检测.结果 TT51大米组与市售和亲本大米组比较,雄性子代体重、食物利用率、血常规、血生化、血清性激素水平以及精子各项指标差异无统计学意义.结论 转Bt基因大米暴露对雄性大鼠子代生殖系统发育未见不良影响.