Neoagarooligosaccharide Protects against Hepatic Fibrosis via Inhibition of TGF-β/Smad Signaling Pathway

Hepatic fibrosis occurs when liver tissue becomes scarred from repetitive liver injury and inflammatory responses; it can progress to cirrhosis and eventually to hepatocellular carcinoma. Previously, we reported that neoagarooligosaccharides (NAOs), produced by the hydrolysis of agar by β-agarases, have hepatoprotective effects against acetaminophen overdose-induced acute liver injury. However, the effect of NAOs on chronic liver injury, including hepatic fibrosis, has not yet been elucidated. Therefore, we examined whether NAOs protect against fibrogenesis in vitro and in vivo. NAOs ameliorated PAI-1, α-SMA, CTGF and fibronectin protein expression and decreased mRNA levels of fibrogenic genes in TGF-β-treated LX-2 cells. Furthermore, downstream of TGF-β, the Smad signaling pathway was inhibited by NAOs in LX-2 cells. Treatment with NAOs diminished the severity of hepatic injury, as evidenced by reduction in serum alanine aminotransferase and aspartate aminotransferase levels, in carbon tetrachloride (CCl₄)-induced liver fibrosis mouse models. Moreover, NAOs markedly blocked histopathological changes and collagen accumulation, as shown by H&E and Sirius red staining, respectively. Finally, NAOs antagonized the CCl₄-induced upregulation of the protein and mRNA levels of fibrogenic genes in the liver. In conclusion, our findings suggest that NAOs may be a promising candidate for the prevention and treatment of chronic liver injury via inhibition of the TGF-β/Smad signaling pathway.     

Ozonation of CI Reactive Black 5 using rotating packed bed and stirred tank reactor

This study investigates the ozonation of CI Reactive Black 5 (RB5) by using the rotating packed bed (RPB) and completely stirred tank reactor (CSTR) as ozone contactors. The RPB, which provides high gravitational force by adjusting the rotational speed, was employed as a novel ozone contactor. The same ozone dosage was separately introduced into either the RPB or the CSTR for the investigation, while the experimental solution was continuously circulated within the apparatus consisting of the RPB and CSTR. The decolorization and mineralization efficiencies of RB5 in the course of ozonation are compared for these two methods. Moreover, the dissolved and off‐gas ozone concentrations were simultaneously monitored for the further analysis. As a result, the ozone mass transfer rate per unit volume of the RPB was significantly higher because of its higher mass transfer coefficient and gas–liquid concentration driving force. Furthermore, ozonation kinetics was found to be independent of the gravitational magnitude of an ozone gas–liquid contactor. Therefore, the results suggest employing RPBs as ozone‐contacting devices with the advantage of volume reduction. The experimental results, which can be used for further modeling of the ozonation process in the RPB, also show the requirement of correct design for the RPB. Consequently, the present study is useful for the understanding of practical application of RPBs. Copyright © 2004 Society of Chemical Industry     

缺血性糖尿病下肢病变的膝下动脉成形术

目的探讨膝下动脉的球囊成形术在治疗糖尿病缺血性下肢病变中的价值。方法对５３例糖尿病合并缺血性下肢病变患者６４侧缺血性下肢进行膝下动脉Deep球囊扩张成形术。对比患者手术前后临床症状的改变及足部溃疡的变化。结果５３例患者共１１９支动脉分支接受PTA治疗，５０例患者的１０１个分支成功地开通，技术成功率为８４．９％，所有５０例患者的临床症状均明显缓解，足部溃疡均变小，无需截肢。结论膝下动脉的球囊成形术是治疗缺血性糖尿病下肢病变安全有效的微创手段     

A study on the deposition of pyrolytic carbons from hydrocarbons

A new method for forming isotropic, laminar, and columnar pyrolytic carbons is proposed. For this, a low RPM (below 2.4 rpm) tumbling bed has been used to deposit pyrolytic carbons from hydrocarbon gases. All deposits were made on graphite substrates from propane and methane at a constant temperature of 1200°C. The microstructures of the pyrolytic carbons deposited were dependent on the flow pattern of the reactant gas, the rpm of the reactor, the hydrocarbon concentration, the nature of the hydrocarbon, and the geometry of the bed. Isotropic pyrolytic carbon is formed under deposition conditions where homogeneous nucleation occurs in the gas phase and at the gas flow conditions where the gas-borne droplets can collide on the substrate. Laminar carbon is formed under deposition conditions where homogeneous nucleation does not occur in the gas phase and at gas flow conditions where the carbon species existing in the bulk of the gas phase can collide on the substrate. Columnar carbon is formed when any carbon products existing in the bulk of the gas phase cannot collide on the substrate. The suggested deposition mechanism can also be applied to pyrolytic carbons deposited in a fluidized bed or in a stationary bed. In particular, isotropic carbon can be obtained even in a stationary bed if the requirements for the deposition of the isotropic carbon described above are satisfied.     

The Complexity of Compressed Membership Problems for Finite Automata

In this paper, a compressed membership problem for finite automata, both deterministic (DFAs) and non-deterministic (NFAs), with compressed transition labels is studied. The compression is represented by straight-line programs (SLPs), i.e. context-free grammars generating exactly one string. A novel technique of dealing with SLPs is employed: the SLPs are recompressed, so that substrings of the input word are encoded in SLPs labelling the transitions of the NFA (DFA) in the same way, as in the SLP representing the input text. To this end, the SLPs are locally decompressed and then recompressed in a uniform way. Furthermore, in order to reflect the recompression in the NFA, we need to modify it only a little, in particular its size stays polynomial in the input size. Using this technique it is shown that the compressed membership for NFA with compressed labels is in NP, thus confirming the conjecture of Plandowski and Rytter (Jewels Are Forever, pp. 262–272, Springer, Berlin, 1999) and extending the partial result of Lohrey and Mathissen (in CSR, LNCS, vol. 6651, pp. 275–288, Springer, Berlin, 2011); as this problem is known to be NP-hard (in Plandowski and Rytter, Jewels Are Forever, pp. 262–272, Springer, Berlin, 1999), we settle its exact computational complexity. Moreover, the same technique applied to the compressed membership for DFA with compressed labels yields that this problem is in P, and this problem is known to be P-hard (in Markey and Schnoebelen, Inf. Process. Lett. 90(1):3–6, 2004; Beaudry et al., SIAM J. Comput. 26(1):138–152, 1997).     

Latent catalyst effects in halogen‐free epoxy molding compounds for semiconductor encapsulation

Latent catalyst effects were investigated to improve the physical properties of halogen‐free epoxy molding compounds (EMCs) for semiconductor encapsulation. In this study, biphenyl‐type resins were used as the epoxy and hardener resin for halogen‐free EMCs to obtain high flame‐retardant properties and high filler contents. Latent catalyst effects were examined with two kinds of EMC compositions, halogen‐free EMCs and conventional EMC compositions. We used triphenylphosphine‐benzoquinone salt (TPP‐BQ) as a latent catalyst. Spiral flow and gel time were measured to investigate the change in moldability with the latent catalyst. We measured package fail, moisture absorption, and delamination for reliability evaluation and flexural strength, flexural modulus, and adhesion for mechanical properties to examine latent catalyst effects. An improvement in moldability, reliability, and the mechanical properties were observed in two types of halogen‐free EMCs with TPP‐BQ as a latent catalyst. These phenomena were seen in conventional EMCs, including o‐cresol novolac epoxy resin. The cure kinetics of these systems were investigated by differential scanning calorimetry with an isothermal approach to explain these phenomena. The results indicate that the improvement in moldability in halogen‐free EMCs with TPP‐BQ was due to the low conversion rate of this system, and the increase in mechanical properties was attributed to the high conversion of curing reaction. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 96: 2287–2299, 2005     

Heavy Electron Superconductors in Terms of Block Spin Phenomenology

We investigate the relationship between superconductivity and spin glasses, which were observed to be coexistent in heavy fermion superconductors (HFSs). We begin by explaining the phase of spin glass using concepts of finite-sized block spin. We then introduce the phase of superconductivity in HFSs as collective Cooper pairing, that is, the pairing of block spins with net spin = 1/2, each of which is comprised of a large number of random spins that together produce a majority spin direction. The superconducting Bardeen-Cooper-Schrieffer (BCS)-type phonon-mediated electron-electron interaction is substituted for the BCS-type electron-electron interaction mediated by phonon-enhanced spin flips previously suggested by us. An effective charge of any value stemming from an electron attached to the flux of an electric field can be referred to as a composite charge. The distinguished difference between ours and the original BCS model is the following: (i) another BCS-type interaction, (ii) bare electrons that are substituted for block spins with net spin = 1/2 and net charge = e in the presence of electric fields, and (iii) Fermi-Dirac distributions that are replaced by a new distribution called as Brillouin distribution.     

Rechargeable Aluminum‐Ion Batteries Based on an Open‐Tunnel Framework

Rechargeable batteries based on an abundant metal such as aluminum with a three‐electron transfer per atom are promising for large‐scale electrochemical energy storage. Aluminum can be handled in air, thus offering superior safety, easy fabrication, and low cost. However, the development of Al‐ion batteries has been challenging due to the difficulties in identifying suitable cathode materials. This study presents the use of a highly open framework Mo_{2.5 + y} VO_{9 + z} as a cathode for Al‐ion batteries. The open‐tunnel oxide allows a facile diffusion of the guest species and provides sufficient redox centers to help redistribute the charge within the local host lattice during the multivalent‐ion insertion, thus leading to good rate capability with a specific capacity among the highest reported in the literature for Al‐based batteries. This study also presents the use of Mo_{2.5 + y} VO_{9 + z} as a model host to develop a novel ultrafast technique for chemical insertion of Al ions into host structures. The microwave‐assisted method employing diethylene glycol and aluminum diacetate (Al(OH)(C₂H₃O₂)₂) can be performed in air in as little as 30 min, which is far superior to the traditional chemical insertion techniques involving moisture‐sensitive organometallic reagents. The Al‐inserted Al _x Mo_{2.5 + y} VO_{9 + z} obtained by the microwave‐assisted chemical insertion can be used in Al‐based rechargeable batteries.