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Ali Raza Wei Su Ang Gao Sundas Saher Mehmood Muhammad Azhar Hussain Wenlong Nie Yan Lv Xiling Zou Xuekun Zhang 《International journal of molecular sciences》2021,22(8)
Catalase (CAT) is an antioxidant enzyme expressed by the CAT gene family and exists in almost all aerobic organisms. Environmental stresses induce the generation of reactive oxygen species (ROS) that eventually hinder plant growth and development. The CAT enzyme translates the hydrogen peroxide (H2O2) to water (H2O) and reduce the ROS levels to shelter the cells’ death. So far, the CAT gene family has not been reported in rapeseed (Brassica napus L.). Therefore, a genome-wide comprehensive analysis was conducted to classify the CAT genes in the rapeseed genome. The current study identified 14 BnCAT genes in the rapeseed genome. Based on phylogenetic and synteny analysis, the BnCATs belong to four groups (Groups I–IV). A gene structure and conserved motif analysis showed that Group I, Group II, and Group IV possess almost the same intron/exon pattern, and an equal number of motifs, while Group III contains diverse structures and contain 15 motifs. By analyzing the cis-elements in the promoters, we identified five hormone-correlated responsive elements and four stress-related responsive elements. Further, six putative bna-miRNAs were also identified, targeting three genes (BnCAT4, BnCAT6, and BnCAT8). Gene ontology (GO) enrichment analysis showed that the BnCAT genes were largely related to cellular organelles, ROS response, stimulus response, stress response, and antioxidant enzymes. Almost 10 BnCAT genes showed higher expression levels in different tissues, i.e., root, leaf, stem, and silique. The expression analysis showed that BnCAT1–BnCAT3 and BnCAT11–BnCAT13 were significantly upregulated by cold, salinity, abscisic acid (ABA), and gibberellic acid (GA) treatment, but not by drought and methyl jasmonate (MeJA). Notably, most of the genes were upregulated by waterlogging stress, except BnCAT6, BnCAT9, and BnCAT10. Our results opened new windows for future investigations and provided insights into the CAT family genes in rapeseed. 相似文献
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Sajid Husain G. S. R. Sastry N. Prasada Raju 《Journal of the American Oil Chemists' Society》1991,68(11):822-826
A simple method for quality assessment of heated oils and fats is described. The proposed method involves precise determination
of molecular weight averages (MWA)viz., the weight average molecular weight (
), the number average molecular weight (
) and the Z-average molecular weight (
) by high performance size exclusion chromatography (HPSEC) and their quantitative correlation to percent polar material obtained
by column chromatography (CC). Change in MWA on heat treatment of fourteen different edible oils and fats at 180° ± 2°C for
eight 8-hr days is studied. Relative standard deviations and regression coefficients of correlation between MWA and their
ratios (
, {
}, {
}, {
}, {
} and {
)vs percent polar material have been reported. Probable discard time was predicted for all the oils based on the above-mentioned
correlations and also from the percent of high molecular weight (H. Mwt) species formed. It was observed that the oils which
are generally recommended for coronary patients deteriorated faster. A possibility of extending this methodology to the frying
oils is suggested.
Part of the work was presented at “40th Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy” at Georgia
World Congress Centre, USA, 1989.
IICT Communication No. 2630. 相似文献
6.
Sajid Husain M. Kifayatullah G. S. R. Sastry N. Prasada Raju 《Journal of the American Oil Chemists' Society》1993,70(12):1251-1254
Application of13C nuclear magnetic resonance (NMR) spectroscopy for detection of castor oil (CO) in various edible oils, such as coconut oil,
palm oil, groundnut oil and mustard oil, is described. Characteristic signals observed at δ 132.4, δ 125.6, δ 71.3, δ 36.8
and δ 35.4 ppm, due to C10, C9, C12, C13 and C11 carbons of ricinoleic acid (RA) in CO, were selected for distinguishing it
from edible oils. Quantitative13C NMR spectra of oils were recorded in CDCl3 with a gated decoupling technique. The minimum detection limits for qualitative and quantitative analyses were 2.0 and 3.0%,
respectively. The proposed method is simple, nondestructive and requires no sample pretreatment. Its application to heat-abused
oils has also been demonstrated successfully without any of the interferences observed in most other methods. 相似文献
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Masoom Alam Xinwen Zhang Mohammad Nauman Tamleek Ali Muhammad Ali Sajid Anwar Quratulain Alam 《Multimedia Tools and Applications》2013,66(2):283-302
Service Oriented Architecture with underlying technologies like web services and web service orchestration opens new vistas for integration among business processes operating in heterogeneous environments. However, such dynamic collaborations require a highly secure environment at each respective business partner site. Existing web services standards address the issue of security only on the service provider platform. The partner platforms to which sensitive information is released have till now been neglected. Remote Attestation is a relatively new field of research which enables an authorized party to verify that a trusted environment actually exists on a partner platform. To incorporate this novel concept in to the web services realm, a new mechanism called WS-Attestation has been proposed. This mechanism provides a structural paradigm upon which more fine-grained solutions can be built. In this paper, we present a novel framework, Behavioral Attestation for Web Services, in which XACML is built on top of WS-Attestation in order to enable more flexible remote attestation at the web services level. We propose a new type of XACML policy called XACML behavior policy, which defines the expected behavior of a partner platform. Existing web service standards are used to incorporate remote attestation at the web services level and a prototype is presented, which implements XACML behavior policy using low-level attestation techniques. 相似文献
9.
Faisal Ahmed Carlos Rodríguez-Fernández Henry A. Fernandez Yi Zhang Abde Mayeen Shafi Md Gius Uddin Xiaoqi Cui Hoon Hahn Yoon Naveed Mehmood Andreas C. Liapis Lide Yao Humeyra Caglayan Zhipei Sun Harri Lipsanen 《Advanced functional materials》2023,33(33):2302051
Developing selective and coherent polymorphic crystals at the nanoscale offers a novel strategy for designing integrated architectures for photonic and optoelectronic applications such as metasurfaces, optical gratings, photodetectors, and image sensors. Here, a direct optical writing approach is demonstrated to deterministically create polymorphic 2D materials by locally inducing metallic 1T′-MoTe2 on the semiconducting 2H-MoTe2 host layer. In the polymorphic-engineered MoTe2, 2H- and 1T′- crystalline phases exhibit strong optical contrast from near-infrared to telecom-band ranges (1–1.5 µm), due to the change in the band structure and increase in surface roughness. Sevenfold enhancement of third harmonic generation intensity is realized with conversion efficiency (susceptibility) of ≈1.7 × 10−7 (1.1 × 10−19 m2 V−2) and ≈1.7 × 10−8 (0.3 × 10−19 m2 V−2) for 1T′ and 2H-MoTe2, respectively at telecom-band ultrafast pump laser. Lastly, based on polymorphic engineering on MoTe2, a Schottky photodiode with a high photoresponsivity of 90 AW−1 is demonstrated. This study proposes facile polymorphic engineered structures that will greatly benefit realizing integrated photonics and optoelectronic circuits. 相似文献
10.
Artificial Life and Robotics - This paper describes the design and performance evaluation of a flexible wearable haptic device that aims to realize full kinesthetic haptic feedback for application... 相似文献