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HM Vu D Myers R de Lorimier TJ Matthews MA Moody C Heinly JV Torres BF Haynes L Spicer 《Canadian Metallurgical Quarterly》1999,73(1):746-750
Immunogenic peptides containing epitopes of the gp120 C4 and V3 regions from human immunodeficiency virus strains MN and EV91 have been studied by nuclear magnetic resonance and molecular modeling and used as immunogens in rhesus monkeys. The results, combined with those for other peptides, suggest a correlation between solution conformation and immunologic cross-reactivity. 相似文献
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BACKGROUND: Macrosclerotherapy in combination with compression has proven to be safe and effective in the treatment of varicose veins. Local compression is increased by pads, according to Laplace law. Firm rolls of cotton wool are fixed over the course of the entire vein to increase local compression and to reduce complications. Additional compression is given by a combination of a class I (daytime and nighttime) and class II (daytime only) medical compression hosiery. PURPOSE: To evaluate the effectiveness and side effects of sclerocompression therapy with cotton wool rolls in combination with medical compression hosiery. METHOD: Prospective study with 100 patients (120 legs) with primary varicose veins, which are treated with polidocanol as sclerosant with the empty vein technique. Immediately after the injection, a long cotton wool roll is placed over the entire vein and fixed. Additional compression is obtained with class I and class II medical compression hosiery. The interface pressure on the skin, just under the cotton wool roll, is measured on 12 legs with the aid of an interface pressure measuring instrument (Oxford Pressure Monitor). RESULTS: Good sclerosing results are obtained in all patients. Side effects are classified as early and late. In 16 patients, minor side effects which needed no treatment are observed. In only 3 cases (2.5%), intravascular blood clots (2) and phlebitis (1) needed incision and expression. The mean interface pressure of all measuring sensors under the cotton wool roll is 84 mm/Hg (68 to 122 mm/Hg). CONCLUSION: This study proves the high effectiveness of a cotton wool roll compression right at the place of treatment. By using these long cotton wool compression rolls, the compression part of sclerocompression therapy becomes more effective and much easier to perform. 相似文献
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Stahl's ear is a congenital malformation of the auricle, which is uncommon in non-Oriental societies. A number of different treatment modalities have been suggested, many of which yield unpredictable results. We review current treatment modalities from the English literature and describe a novel, simple surgical method of repair. First, a helical rim incision is made to expose the abnormal third crus and upper pole of the ear. The third crus is excised as a narrow wedge of cartilage and posterior skin, and the defect is closed primarily. The free third crus cartilage is then grafted onto the scaphal cartilage in an anatomically correct position to form a superior crus. The anterior skin flap is redraped and bolstered over this graft. Only a very small scar on the helical rim remains visible anteriorly. 相似文献
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The article focuses on the undesirable binary between the rural and the urban and uses Company Drinks as an example of a reverse ruralurban relationship, where an inner city population migrates temporarily to the countryside, which is relevant to the hop-picking tradition (“hopping”) practiced by working-class families from London’s East End. In this case, a working-class community has developed its own rural practice, moving between rural and urban settings on a regular basis. Company Drinks is a new model cultural enterprise that uses the collective memory of hopping as a starting point to rethink and reintroduce an adaptive collective production cycle into East London everyday life. With various successful activities being held, going picking has been far from an East End tradition and become a universal activity recognized and appreciated across different cultures and landscapes. The author believes that rural-urban link cannot be controlled from a distance. Rural society offers knowledge and resource that can empower urban communities, and can test and provide conditions for alternatives to urban lifestyles. 相似文献
6.
Structural studies on an inhibitory antibody against Thermus aquaticus DNA polymerase suggest mode of inhibition 总被引:1,自引:0,他引:1
Murali R; Helmer-Citterich M; Sharkey DJ; Scalice ER; Daiss JL; Sullivan MA; Krishna Murthy HM 《Protein engineering, design & selection : PEDS》1998,11(2):79-86
TP7, an antibody against Thermus aquaticus DNA polymerase I (TaqP), is used
as a thermolabile switch in 'hot start' variations of PCR to minimize
non-specific amplification events. Earlier studies have established that
TP7 binds to the polymerase domain of TaqP, competes with primer template
complex for binding and is a potent inhibitor of the polymerase activity of
TaqP. We report crystallographic determination of the structure of an Fab
fragment of TP7 and computational docking of the structure with the known
three-dimensional structure of the enzyme. Our observations strongly
suggest that the origin of inhibitory ability of TP7 is its binding to
enzyme residues involved in DNA binding and polymerization mechanism.
Although criteria unbiased by extant biochemical data have been used in
identification of a putative solution, the resulting complex offers an
eminently plausible structural explanation of biochemical observations. The
results presented are of general significance for interpretation of docking
experiments and in design of small molecular inhibitors of TaqP, that are
not structurally similar to substrates, for use in PCR. Structural and
functional similarities noted among DNA polymerases, and the fact that
several DNA polymerases are pharmacological targets, make discovery of
non-substrate based inhibitors of additional importance.
相似文献
7.
M Sadowski HM Wisniewski M Tarnawski PB Kozlowski B Lach J Wegiel 《Canadian Metallurgical Quarterly》1999,97(2):156-164
OBJECTIVE: To examine directly the extent to which ICD-10 hyperkinetic disorder and DSM-IV attention-deficit/hyperactivity disorder (ADHD) identify the same children with the same difficulties. METHOD: Participants were children referred for symptoms of overactivity, inattention, and impulsivity, and a normal control group. Diagnostic criteria for ICD-10 hyperkinetic disorder and DSM-IV ADHD were applied retrospectively. Four groups were identified: hyperkinetic disorder and ADHD (n = 21), ADHD only (n = 22), clinic control (n = 15), and normal control (n = 19). The groups were compared on measures reflecting the central characteristics of ADHD, neurodevelopmental functioning, academic and cognitive functioning, and the presence of conduct problems. RESULTS: There is some evidence of increased symptom severity in the combined diagnostic group. Few differences emerged on measures of neurodevelopmental, academic, and cognitive functioning. Rates of conduct disturbance were similar in both ADHD groups. CONCLUSIONS: DSM-IV criteria identify a broader group of children than those identified by ICD-10. However, there is substantial overlap between the groups formed with these different criteria. 相似文献
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