全文获取类型
收费全文 | 556篇 |
免费 | 29篇 |
国内免费 | 1篇 |
专业分类
电工技术 | 10篇 |
综合类 | 2篇 |
化学工业 | 142篇 |
金属工艺 | 21篇 |
机械仪表 | 7篇 |
建筑科学 | 33篇 |
矿业工程 | 2篇 |
能源动力 | 15篇 |
轻工业 | 49篇 |
水利工程 | 2篇 |
石油天然气 | 2篇 |
无线电 | 33篇 |
一般工业技术 | 112篇 |
冶金工业 | 98篇 |
原子能技术 | 2篇 |
自动化技术 | 56篇 |
出版年
2023年 | 8篇 |
2022年 | 7篇 |
2021年 | 30篇 |
2020年 | 21篇 |
2019年 | 22篇 |
2018年 | 13篇 |
2017年 | 22篇 |
2016年 | 25篇 |
2015年 | 12篇 |
2014年 | 26篇 |
2013年 | 33篇 |
2012年 | 30篇 |
2011年 | 22篇 |
2010年 | 24篇 |
2009年 | 24篇 |
2008年 | 18篇 |
2007年 | 22篇 |
2006年 | 15篇 |
2005年 | 14篇 |
2004年 | 14篇 |
2003年 | 15篇 |
2002年 | 13篇 |
2001年 | 15篇 |
2000年 | 5篇 |
1999年 | 5篇 |
1998年 | 18篇 |
1997年 | 12篇 |
1996年 | 16篇 |
1995年 | 11篇 |
1994年 | 8篇 |
1993年 | 7篇 |
1990年 | 6篇 |
1989年 | 3篇 |
1988年 | 4篇 |
1986年 | 5篇 |
1985年 | 4篇 |
1984年 | 3篇 |
1983年 | 3篇 |
1981年 | 5篇 |
1980年 | 3篇 |
1978年 | 3篇 |
1976年 | 6篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1971年 | 2篇 |
1967年 | 1篇 |
1966年 | 1篇 |
1942年 | 1篇 |
1927年 | 1篇 |
排序方式: 共有586条查询结果,搜索用时 31 毫秒
1.
Ruirui Lu Katharina Metzner Fangyuan Zhou Cathrin Flauaus Annika Balzulat Patrick Engel Jonas Petersen Rebekka Ehinger Anne Bausch Peter Ruth Robert Lukowski Achim Schmidtko 《International journal of molecular sciences》2021,22(1)
The sodium-activated potassium channel Slack (KNa1.1, Slo2.2, or Kcnt1) is highly expressed in populations of sensory neurons, where it mediates the sodium-activated potassium current (IKNa) and modulates neuronal activity. Previous studies suggest that Slack is involved in the processing of neuropathic pain. However, mechanisms underlying the regulation of Slack activity in this context are poorly understood. Using whole-cell patch-clamp recordings we found that Slack-mediated IKNa in sensory neurons of mice is reduced after peripheral nerve injury, thereby contributing to neuropathic pain hypersensitivity. Interestingly, Slack is closely associated with ATP-sensitive P2X3 receptors in a population of sensory neurons. In vitro experiments revealed that Slack-mediated IKNa may be bidirectionally modulated in response to P2X3 activation. Moreover, mice lacking Slack show altered nocifensive responses to P2X3 stimulation. Our study identifies P2X3/Slack signaling as a mechanism contributing to hypersensitivity after peripheral nerve injury and proposes a potential novel strategy for treatment of neuropathic pain. 相似文献
2.
Maryam Oroujeni Sara S. Rinne Anzhelika Vorobyeva Annika Loftenius Joachim Feldwisch Per Jonasson Vladimir Chernov Anna Orlova Fredrik Y. Frejd Vladimir Tolmachev 《International journal of molecular sciences》2021,22(5)
Radionuclide imaging of HER2 expression in tumours may enable stratification of patients with breast, ovarian, and gastroesophageal cancers for HER2-targeting therapies. A first-generation HER2-binding affibody molecule [99mTc]Tc-ZHER2:V2 demonstrated favorable imaging properties in preclinical studies. Thereafter, the affibody scaffold has been extensively modified, which increased its melting point, improved storage stability, and increased hydrophilicity of the surface. In this study, a second-generation affibody molecule (designated ZHER2:41071) with a new improved scaffold has been prepared and characterized. HER2-binding, biodistribution, and tumour-targeting properties of [99mTc]Tc-labelled ZHER2:41071 were investigated. These properties were compared with properties of the first-generation affibody molecules, [99mTc]Tc-ZHER2:V2 and [99mTc]Tc-ZHER2:2395. [99mTc]Tc-ZHER2:41071 bound specifically to HER2 expressing cells with an affinity of 58 ± 2 pM. The renal uptake for [99mTc]Tc-ZHER2:41071 and [99mTc]Tc-ZHER2:V2 was 25–30 fold lower when compared with [99mTc]Tc-ZHER2:2395. The uptake in tumour and kidney for [99mTc]Tc-ZHER2:41071 and [99mTc]Tc-ZHER2:V2 in SKOV-3 xenografts was similar. In conclusion, an extensive re-engineering of the scaffold did not compromise imaging properties of the affibody molecule labelled with 99mTc using a GGGC chelator. The new probe, [99mTc]Tc-ZHER2:41071 provided the best tumour-to-blood ratio compared to HER2-imaging probes for single photon emission computed tomography (SPECT) described in the literature so far. [99mTc]Tc-ZHER2:41071 is a promising candidate for further clinical translation studies. 相似文献
3.
Douglas Weber David Patsch Annika Neumann Dr. Margit Winkler Prof. Dr. Dörte Rother 《Chembiochem : a European journal of chemical biology》2021,22(10):1823-1832
Accessing aldehydes from carboxylate moieties is often a challenging task. In this regard, carboxylate reductases (CARs) are promising catalysts provided by nature that are able to accomplish this task in just one step, avoiding over-reduction to the alcohol product. However, the heterologous expression of CARs can be quite difficult due to the excessive formation of insoluble protein, thus hindering further characterization and application of the enzyme. Here, the heterologous production of the carboxylate reductase from Nocardia otitidiscaviarum (NoCAR) was optimized by a combination of i) optimized cultivation conditions, ii) post-translational modification with a phosphopantetheinyl transferase and iii) selection of an appropriate expression strain. Especially, the selection of Escherichia coli tuner cells as host had a strong effect on the final 110-fold increase in the specific activity of NoCAR. This highly active NoCAR was used to reduce sodium benzoate to benzaldehyde, and it was successfully assembled with an in vitro regeneration of ATP and NADPH, being capable of reducing about 30 mM sodium benzoate with high selectivity in only 2 h of reaction. 相似文献
4.
5.
Annika Linke Tobias Linke Reinhard Kohlus 《European Journal of Lipid Science and Technology》2020,122(8)
Microencapsulation aims to protect polyunsaturated fatty acids against oxidation by embedding oil droplets in a solid matrix. In such a system the internal (dissolved and entrapped) and external (in the environment) oxygen can be differentiated. The study aims to quantify the impact of both oxygen sources on the oxidation of microencapsulated fish oil. The impact of the solubilized oxygen in bulk fish oil is investigated by saturating the oil with nitrogen, synthetic air, and pure oxygen. Even though more dissolved oxygen results in more oxidation products, the difference between the oxidation of the nitrogen and air saturated oil is significant but low. For encapsulated fish oil powders, the internal oxygen is modified by preparing oil‐in‐water emulsions under atmospheric and inert conditions. The feed is atomized and spray dried with either nitrogen or air. Powders are stored under vacuum and in vials and the hydroperoxides and anisidine value are determined in the total‐ and encapsulated oil. The internal oxygen has a minor impact, whereas the external oxygen is the main determinant for autoxidation. Apart from oxidizing the non‐encapsulated oil, the external O2 penetrates into the particle and reacts with the encapsulated oil. Practical Applications: Comparing the contribution of the internal and external oxygen to the oxidative stability shows that the internal O2 plays a minor role and can be neglected. This means that the emulsion preparation as well as the spray drying process can be conducted under ambient conditions. An inert production is not extending the shelf life significantly as long as the external O2 determines oxidation. The focus should be on optimizing the diffusion barrier properties of the wall matrix to reduce the penetration of the external oxygen into the particle system. Alternatively, packaging solution reducing the external O2 will extend the shelf life of the microencapsulated oil. 相似文献
6.
Annika Jahn Anton Hoffmann Luisa Blaesing Fabian Kunde Martin Bertau Martina Bremer Steffen Fischer 《化学,工程师,技术》2020,92(11):1733-1740
The enzymatic conversion of lignins, possibly in combination with electrochemical oxidation, makes aromatics such as syringol, guaiacol, vanillin and catechol available in the qualities required by the fragrance industry. The lignins were obtained by soda digestion from wheat straw and Miscanthus, characterized and then converted with laccases. The overall yield amounted up to 9 wt % with a product spectrum confined to four substances. Catechol was the major product, with a fraction of ≈75 %. It can easily be isolated by extraction with acetone. 相似文献
7.
Dr. Santina Maiorana Prof. Roberta Ettari Dr. Santo Previti Dr. Giorgio Amendola Dr. Annika Wagner Prof. Sandro Cosconati Prof. Ute A. Hellmich Prof. Tanja Schirmeister Prof. Maria Zappalà 《ChemMedChem》2020,15(16):1552-1561
In this paper, we report the design, synthesis and biological investigation of a series of peptidyl vinyl ketones obtained by modifying the P2 fragment of previously reported highly potent inhibitors of rhodesain, the main cysteine protease of Trypanosoma brucei rhodesiense. Investigation of the structure–activity relationship led us to identify new rhodesain inhibitors endowed with an improved selectivity profile (a selectivity index of up to 22 000 towards the target enzyme), and/or an improved antitrypanosomal activity in the sub-micromolar range. 相似文献
8.
Annika K. E. Andersson Sverre M. Selbach Christopher S. Knee Tor Grande 《Journal of the American Ceramic Society》2014,97(8):2654-2661
The crystal structures of proton‐conducting BaZr1?xYxO3?x/2 (BZY05–BZY20) and BaCe0.8Y0.2O2.9 (BCY20) during hydration/dehydration has been studied by in situ high‐temperature X‐ray diffraction and thermal analysis. A contraction/expansion of the crystal lattice associated with dehydration/hydration was observed for all materials at elevated temperatures and the polymorphic phase transition temperatures of BaCe0.8Y0.2O2.9 were depressed by lowering the vapor pressure of water. A thermodynamic formalism is introduced to describe the chemical expansion associated with the hydration of oxygen vacancies in acceptor‐doped oxides. A conventional point defect model was applied to describe the lattice strain associated with the hydration. The chemical expansion is discussed with respect to the available volumetric data on the hydration of proton‐conducting oxide materials and its likely impact on ceramic fuel cells/hydrogen separation membranes utilizing a proton‐conducting electrolyte. 相似文献
9.
10.
Dr. Carolyn Vargas Prof. Dr. Gerald Radziwill Dr. Gerd Krause Dr. Anne Diehl Prof. Dr. Sandro Keller Dr. Nestor Kamdem Prof. Dr. Constantin Czekelius Annika Kreuchwig Dr. Peter Schmieder Dr. Declan Doyle Prof. Dr. Karin Moelling Dr. Volker Hagen Dr. Markus Schade Prof. Dr. Hartmut Oschkinat 《ChemMedChem》2014,9(7):1458-1462
PDZ (PSD‐95, Dlg, ZO‐1) domains are ubiquitous interaction modules that are involved in many cellular signal transduction pathways. Interference with PDZ‐mediated protein–protein interactions has important implications in disease‐related signaling processes. For this reason, PDZ domains have gained attention as potential targets for inhibitor design and, in the long run, drug development. Herein we report the development of small molecules to probe the function of the PDZ domain from human AF6 (ALL1‐fused gene from chromosome 6), which is an essential component of cell–cell junctions. These compounds bind to AF6 PDZ with substantially higher affinity than the peptide (Ile‐Gln‐Ser‐Val‐Glu‐Val) derived from its natural ligand, EphB2. In intact cells, the compounds inhibit the AF6–Bcr interaction and interfere with epidermal growth factor (EGF)‐dependent signaling. 相似文献