Precision and accuracy in the quantitative analysis of biological samples by accelerator mass spectrometry: application in microdose absolute bioavailability studies

Determination of the pharmacokinetics and absolute bioavailability of an experimental compound, SCH 900518, following a 89.7 nCi (100 μg) intravenous (iv) dose of (14)C-SCH 900518 2 h post 200 mg oral administration of nonradiolabeled SCH 900518 to six healthy male subjects has been described. The plasma concentration of SCH 900518 was measured using a validated LC-MS/MS system, and accelerator mass spectrometry (AMS) was used for quantitative plasma (14)C-SCH 900518 concentration determination. Calibration standards and quality controls were included for every batch of sample analysis by AMS to ensure acceptable quality of the assay. Plasma (14)C-SCH 900518 concentrations were derived from the regression function established from the calibration standards, rather than directly from isotopic ratios from AMS measurement. The precision and accuracy of quality controls and calibration standards met the requirements of bioanalytical guidance (U.S. Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research, Center for Veterinary Medicine. Guidance for Industry: Bioanalytical Method Validation (ucm070107), May 2001. http://www.fda.gov/downloads/Drugs/GuidanceCompilanceRegulatoryInformation/Guidances/ucm070107.pdf ). The AMS measurement had a linear response range from 0.0159 to 9.07 dpm/mL for plasma (14)C-SCH 900158 concentrations. The CV and accuracy were 3.4-8.5% and 94-108% (82-119% for the lower limit of quantitation (LLOQ)), respectively, with a correlation coefficient of 0.9998. The absolute bioavailability was calculated from the dose-normalized area under the curve of iv and oral doses after the plasma concentrations were plotted vs the sampling time post oral dose. The mean absolute bioavailability of SCH 900518 was 40.8% (range 16.8-60.6%). The typical accuracy and standard deviation in AMS quantitative analysis of drugs from human plasma samples have been reported for the first time, and the impact of these parameters on quantitative analysis was further assessed using the Z factor. The use of the lowest achievable LLOQ(Z=0) derived from statistical analysis of the control and low-concentration standards for AMS measurements is proposed in future studies.     

Additive-free photosynthesis of acrylamide hydrogels initiated with CdS and TiO<Subscript>2</Subscript> as light visible nano-photocatalysts

Hydrogels are three-dimensional polymer networks which can be synthesized by different techniques, such as free-radical addition reaction which is the most well-known technique in functional vinyl monomer polymerization. Photopolymerization, as an attractive technique, has been used in radical polymerization of monomers and has revitalized interest in the idea that it congregates a wide range of economic and ecological expectations. Due to the spatial–temporal control and a mild curing process of polymerization, photoinitiator semiconductor nano-particles offer great advantages, such as effective and quantitative reaction. Cadmium sulfide nanowire, titanium dioxide (TiO₂) nanotube, and TiO₂ nanowire were used as visible photocatalysts for photopolymerization of acrylamide hydrogel without using any additive under the sunlight and purple LED irradiations. The effects of different synthetic parameters, including initiator type and concentration and type of light sources, were investigated to achieve hydrogels with maximum swelling capacity. The results showed that the swelling of hydrogel reached 80 g water/g hydrogel when the TiO₂ nanowire was used as the photoinitiator. The synthesized semiconductors and hydrogels were characterized by X-ray diffractometry, adsorption isotherm, infrared spectroscopy, thermogravimetric analysis, and transmission electron microscopy. According to the results, the new initiators led to semiconductor-based hydrogels, achieved with high swelling property through a high-speed high-efficient photopolymerization reaction in a safe manner.     

SO2 Removal from Gas Streams by Ammonia Scrubbing: Process Optimization by Response Surface Methodology

Reduction of the SO₂ content by ammonia scrubbing in the incinerator of the Isfahan Refinery complex was investigated. An experimental continuous setup was designed to study the underlying process parameters affecting SO₂ capture from air. The effects of various parameters was analyzed by a systematic experimental design based on response surface methodology with central composite design. The developed response surface model was found to be useful and robust to predict the degree of desulfurization of ammonia wet flow gas disulfurization reactors. The ammonia concentration had the most significant influence on the efficiency of the desulfurization process. The square of temperature affected the efficiency more than the temperature, whereas the gas flow rate had a minor influence on the separation efficiency.     

Simultaneous removal of gaseous pollutants with a novel swirl wet scrubber

In recent years, researchers have put a considerable effort to decrease the emission of harmful gaseous pollutants to the atmosphere. Although conventional wet scrubbers are being widely used to remove harmful gases, they have low removal efficiencies. This study reports the effect of some operating conditions on simultaneous removal of NO, NO₂, SO₂, and CO₂, using a novel swirl wet scrubber system. The gaseous pollutants were absorbed into NaOH solution. As the absorbent media was circulated continuously for removal purpose, therefore the production of chemical wastes were minimized. The effect of absorbent concentration, gas flow rate, and liquid flow rate were investigated. The best efficiencies of NO, NO₂, SO₂, and CO₂ simultaneous removal were 77, 88, 100 and 80%, respectively, with 2%, w/v NaOH as the scrubber medium. A comparison between this study and literature data shows that the liquid-gas flow rate ratio (F_L/F_G) in the novel scrubber of this work is much smaller than other gaseous pollutant removal systems. Therefore higher removal efficiency is obtained based on the same liquid flow rates.     

Rheological modeling of plug‐assist thermoforming

Solving problems for thermoforming processes in the production of axisymmetric thin walled plastics is investigated in this research work. A nonlinear viscoelastic rheological model with a new strain energy function is suggested for improvement of physical properties of final product. For model validation, a quantitative relation between stress and technical parameters of plug‐assist thermoforming is determined by comparison of theoretical and experimental results. This process with the proposed rheological model could be suggested for prevention from some technical defects such as wall thickness variations, physical instability during inflation‐shrinkage, and warpage exhibited in the final part of a polymeric sheet thermoforming. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 101: 4148–4152, 2006     

Hydrolytic degradation of poly(ethylene terephthalate)

Molecular weight is an important factor in the processing of polymer materials, and it should be well controlled to obtain desired physical properties in final products for end‐use applications. Degradation processes of all kinds, including hydrolytic, thermal, and oxidative degradations, cause chain scission in macromolecules and a reduction in molecular weight. The main purpose of this research is to illustrate the importance of degradation in the drying of poly(ethylene terephthalate) (PET) before processing and the loss of weight and mechanical properties in textile materials during washing. Several techniques were used to investigate the hydrolytic degradation of PET and its effect on changes in molecular weight. Hydrolytic conditions were used to expose fiber‐grade PET chips in water at 85°C for different periods of time. Solution viscometry and end‐group analysis were used as the main methods for determining the extent of degradation. The experimental results show that PET is susceptible to hydrolysis. Also, we that as the time of retention in hydrolytic condition increased, the molecular weight decreases, but the rate of chain cleavage decreased to some extent, at which point there was no more sensible degradation. The obtained moisture content data confirmed the end‐group analysis and viscometry results. Predictive analytical relationships for the estimation of the extent of degradation based on solution viscosity and end‐group analysis are presented. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 103: 2304–2309, 2007     

Water quality index as a simple indicator of watersheds pollution in southwestern part of Iran

The Karun River is the most important watershed in the southwestern region of Iran. Water quality parameters were sampled from October 2006 through July 2007 at three sites along a 4 km gradient, covering both the wet and the dry season. Surface water was collected at three different stations per site (close to the banks and in the middle of the river) and analysed for 14 parameters and heavy metals. The values of 1300, 196.8 and 4042.9 ppm for chemical oxygen demand, biochemical oxygen demand and chloride, respectively, were higher than the standards limits. water quality index (WQI) values were very useful for the classification of the waters monitored. The averaged WQI was low (47%), and quality declined significantly during the dry season [analysis of variance (ANOVA, P < 0.05)]. The annual WQI values of 54.60, 40.29 and 45.71 from sites 1, 2 and 3 correspond to medium, bad and bad water qualities, respectively.     

Strength enhancement of clay by encapsulating geogrids in thin layers of sand

Large size direct shear tests (i.e.300 × 300 × 200 mm) were conducted to investigate the possibility of strength enhancement of clays reinforced with geogrids embedded in thin layers of sand. In this paper test results for the clay, sand, clay–sand, clay–geogrid, sand–geogrid and clay–sand–geogrid samples are presented and discussed. Thin sand layers with thicknesses of 4, 6, 8, 10, 12 and 14 mm were used to quantify their effect on the interaction between the clay and the geogrids. In this regard effects of sand layer thickness, normal pressure (i.e. confinement) and transversal members of geogrids were investigated. All the tests were conducted using saturated clay with no drainage allowed. Test results indicate that provision of thin layers of sand for encapsulating the geogrids is very effective in improving the strength and deformation characteristics of saturated clay. Maximum strength enhancement was derived at an optimum sand layer thickness of 10 mm which proved to be independent of the magnitude of the normal pressure used. For a particular sand layer thickness, increasing the normal pressure resulted in enhanced strength improvement. Results also showed that removal of the geogrid transversal members resulted in reducing the strength of the reinforced samples by 10% compared to geogrids with transversal members. Encapsulating geogrids in thin layers of sand not only will improve the performance of clays if used as backfill it would also provide drainage paths preventing pore water pressure generation on saturation of the backfill.     

Viscosity and rheological behavior of castor–canola mixture

In this work, the effects of shear rate on viscosity of selected pure food grade oils (castor and canola) are investigated. The rheological behavior of castor–canola oil mixture is also studied through modeling the experimental data. The effect of shear rate on the variation of viscosity was clearly observed. Interpretations of the results from rheological models indicate that these food grade oils belong to pseudo‐plastic category. The results also show that among the six used mixing rules, Grunberg–Nissan model seems very well fitted to the experimental data.