Exergy analysis of the diesel pre-reforming solid oxide fuel cell system with anode off-gas recycling in the SchIBZ project. Part I: Modeling and validation

Solid oxide fuel cell (SOFC) systems with anode off-gas recirculation (AGR) and diesel pre-reforming are advantageous because they can operate with the current fuel infrastructure. In the SchIBZ-project, the prototype of such a SOFC system for maritime applications has already been commissioned. In this first paper, we model the system devices to conduct an exergy analysis of this real SOFC plant and validate them with experimental values from experiments in laboratory scale. The results of our simulation agree well with the experimental values. The calculations with the validated results may be closer to the real thermodynamic behavior of such system components than previous literature.     

The Bark Beetle Dendroctonus rhizophagus (Curculionidae: Scolytinae) Has Digestive Capacity to Degrade Complex Substrates: Functional Characterization and Heterologous Expression of an α-Amylase

Dendroctonus-bark beetles are natural agents contributing to vital processes in coniferous forests, such as regeneration, succession, and material recycling, as they colonize and kill damaged, stressed, or old pine trees. These beetles spend most of their life cycle under stem and roots bark where they breed, develop, and feed on phloem. This tissue is rich in essential nutrients and complex molecules such as starch, cellulose, hemicellulose, and lignin, which apparently are not available for these beetles. We evaluated the digestive capacity of Dendroctonus rhizophagus to hydrolyze starch. Our aim was to identify α-amylases and characterize them both molecularly and biochemically. The findings showed that D. rhizophagus has an α-amylase gene (AmyDr) with a single isoform, and ORF of 1452 bp encoding a 483-amino acid protein (53.15 kDa) with a predicted signal peptide of 16 amino acids. AmyDr has a mutation in the chlorine-binding site, present in other phytophagous insects and in a marine bacterium. Docking analysis showed that AmyDr presents a higher binding affinity to amylopectin compared to amylose, and an affinity binding equally stable to calcium, chlorine, and nitrate ions. AmyDr native protein showed amylolytic activity in the head-pronotum and gut, and its recombinant protein, a polypeptide of ~53 kDa, showed conformational stability, and its activity is maintained both in the presence and absence of chlorine and nitrate ions. The AmyDr gene showed a differential expression significantly higher in the gut than the head-pronotum, indicating that starch hydrolysis occurs mainly in the midgut. An overview of the AmyDr gene expression suggests that the amylolytic activity is regulated through the developmental stages of this bark beetle and associated with starch availability in the host tree.     

Bioinformatic characterization of the extracellular lipases from Kluyveromyces marxianus

Lipases are hydrolytic enzymes that break the ester bonds of triglycerides, generating free fatty acids and glycerol. Extracellular lipase activity has been reported for the nonconventional yeast Kluyveromyces marxianus, grown in olive oil as a substrate, and the presence of at least eight putative lipases has been detected in its genome. However, to date, there is no experimental evidence on the physiological role of the putative lipases nor their structural and catalytic properties. In this study, a bioinformatic analysis of the genes of the putative lipases from K. marxianus L-2029 was performed, particularly identifying and characterizing the extracellular expected enzymes, due to their biotechnological relevance. The amino acid sequence of 10 putative lipases, obtained by in silico translation, ranged between 389 and 773 amino acids. Two of the analysed putative proteins showed a signal peptide, 25 and 33 amino acids long for KmYJR107Wp and KmLIP3p, and a molecular weight of 44.53 and 58.23 kDa, respectively. The amino acid alignment of KmLIP3p and KmYJR107Wp with the crystallized lipases from a patatin and the YlLip2 lipase from Yarrowia lipolytica, respectively, revealed the presence of the hydrolase characteristic motifs. From the 3D models of putative extracellular K. marxianus L-2029 lipases, the conserved pentapeptide of each was determined, being GTSMG for KmLIP3p and GHSLG for KmYJR107Wp; besides, the genes of these two enzymes (LIP3 and YJR107W) are apparently regulated by oleate response elements. The phylogenetic analysis of all K. marxianus lipases revealed evolutionary affinities with lipases from abH15.03, abH23.01, and abH23.02 families.     

Structural models of critical regions in old-type r.c. frames with smooth rebars

Structural assessment of existing reinforced concrete constructions under gravity loads and seismic actions has a high social and economical impact; actually in many European countries, most of the buildings dates back to 1960s and 1970s and cannot ensure satisfactory seismic response, since many areas have been later classified as seismic or since design has been carried out according to obsolete codes. These structures are generally reinforced with smooth bars that exhibit poor bond and need specific anchoring end details. In the present paper, some key aspects of structural models of smooth reinforcement for old-type r.c. frame analysis are reported. Results of experimental tests on smooth reinforcement and circular hook anchoring devices are also used to discuss some aspects of behavioural models of beam to column critical regions.     

Performance of “Agave lecheguilla” natural fiber in portland cement composites exposed to severe environment conditions

The main objective of the present research was to provide the housing alternatives to istle zone rural areas in México, which represent about 10% of the national territory. The proposed solution involved a sustainable portland cement-based composite material, reinforced with high tensile strength natural fibers of “Agave lecheguilla”.     

Rapid physicochemical characterization of innovative fucoidan/fructan powders by ATR–FTIR

Functional food has been highly demanded lately because of its benefits in counteracting diseases. Fucoidan and agave fructan are ingredients that enhance the growth of beneficial bacteria in the gut (prebiotics). This mixture has great potential to develop innovative products but it has never been explored before. Because of fucoidan is more expensive than agave fructan, the innovative proposed mixture is vulnerable to adulteration. This research was aimed to assess the accuracy of Fourier transform infrared spectroscopy with attenuated total reflectance (ATR–FTIR) coupled with chemometrics to identify and predict concentration of both polysaccharides in powder mixtures (0–100%). Absorption bands at 1240–1255 and 836–840 cm^?1 were attributed to fucoidan and a strong peak at ~ 936 cm^?1 confirmed the fructan presence. Peak areas were best fitted into linear models (\({\text{R}}_{\text{adj}}^{2}\) ≥ 0.92, RMSE ≤ 3.54%). This achievement may be useful to certificate ingredients contained in fucoidan–fructan mixtures, preventing adulteration.     

Urban Imaginaries: Locating the Modern City

In this article, we review three microcomputer programs for assessing regional economic impacts. The programs are ADOTMATR, GRIMP, and REMI, which we discuss in terms of their suitability for policy analysis and planning practice. We assess the programs in terms of range of applicability, flexibility and ease of use in conducting impact analyses, quality of output, and cost. ADOTMATR and GRIMP are similar; however, GRIMP is easier to use but less flexible and more limited than ADOTMATR. Although REMI is more sophisticated than the other two programs, it is more expensive and more limited in application.     

Review and selection of EPCM as TES materials for building applications

In order to improve the thermal efficiency of building thermal energy storage (TES) systems, the feasibility of using encapsulated phase change materials (EPCMs) as heat storage media is analysed in this work. Specifically, the finite element method is used to perform thermal behaviour analyses of several EPCMs. These analyses include technical and economic assessments in order to identify the best combination of PCM and shell material, using as main parameters: thermal energy storage, heat transfer rate, materials cost, among others. The results show that EPCMs composed by Na₂SO₄·6H₂O as PCM and covered by stainless steel highlight as TES materials.