全文获取类型
收费全文 | 336篇 |
免费 | 10篇 |
国内免费 | 2篇 |
专业分类
电工技术 | 2篇 |
综合类 | 12篇 |
化学工业 | 68篇 |
金属工艺 | 7篇 |
机械仪表 | 5篇 |
建筑科学 | 5篇 |
能源动力 | 3篇 |
轻工业 | 17篇 |
无线电 | 33篇 |
一般工业技术 | 61篇 |
冶金工业 | 86篇 |
原子能技术 | 3篇 |
自动化技术 | 46篇 |
出版年
2022年 | 2篇 |
2021年 | 8篇 |
2020年 | 7篇 |
2019年 | 6篇 |
2018年 | 2篇 |
2017年 | 3篇 |
2016年 | 11篇 |
2015年 | 9篇 |
2014年 | 9篇 |
2013年 | 6篇 |
2012年 | 7篇 |
2011年 | 20篇 |
2010年 | 13篇 |
2009年 | 12篇 |
2008年 | 12篇 |
2007年 | 14篇 |
2006年 | 8篇 |
2005年 | 13篇 |
2004年 | 2篇 |
2003年 | 9篇 |
2002年 | 4篇 |
2001年 | 3篇 |
2000年 | 8篇 |
1999年 | 6篇 |
1998年 | 13篇 |
1997年 | 9篇 |
1996年 | 8篇 |
1995年 | 11篇 |
1994年 | 7篇 |
1993年 | 9篇 |
1992年 | 6篇 |
1991年 | 6篇 |
1990年 | 4篇 |
1989年 | 4篇 |
1988年 | 7篇 |
1987年 | 2篇 |
1986年 | 6篇 |
1985年 | 5篇 |
1984年 | 11篇 |
1983年 | 12篇 |
1982年 | 8篇 |
1981年 | 2篇 |
1980年 | 3篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1977年 | 3篇 |
1976年 | 5篇 |
1975年 | 2篇 |
1974年 | 1篇 |
1972年 | 6篇 |
排序方式: 共有348条查询结果,搜索用时 15 毫秒
1.
Bernette Maria Oosterlaken Heiner Friedrich Gijsbertus de With 《Microscopy research and technique》2022,85(1):412-417
Transmission electron microscopy (TEM) is an important analysis technique to visualize (bio)macromolecules and their assemblies, including collagen fibers. Many protocols for TEM sample preparation of collagen involve one or more washing steps to remove excess salts from the dispersion that could hamper analysis when dried on a TEM grid. Such protocols are not standardized and washing times as well as washing solvents vary from procedure to procedure, with each research group typically having their own protocol. Here, we investigate the influence of washing with water, ethanol, but also methanol and 2-propanol, for both mineralized and unmineralized collagen samples via a protocol based on centrifugation. Washing with water maintains the hydrated collagen structure and the characteristic banding pattern can be clearly observed. Conversely, washing with ethanol results in dehydration of the fibrils, often leading to aggregation of the fibers and a less obvious banding pattern, already within 1 min of ethanol exposure. As we show, this process is fully reversible. Similar observations were made for methanol and propanol. Based on these results, a standardized washing protocol for collagenous samples is proposed. 相似文献
2.
Mikhail V. Fofanov Dmitry Yu. Prokopov Heiner Kuhl Manfred Schartl Vladimir A. Trifonov 《International journal of molecular sciences》2020,21(24)
MicroRNAs play a crucial role in eukaryotic gene regulation. For a long time, only little was known about microRNA-based gene regulatory mechanisms in polyploid animal genomes due to difficulties of polyploid genome assembly. However, in recent years, several polyploid genomes of fish, amphibian, and even invertebrate species have been sequenced and assembled. Here we investigated several key microRNA-associated genes in the recently sequenced sterlet (Acipenser ruthenus) genome, whose lineage has undergone a whole genome duplication around 180 MYA. We show that two paralogs of drosha, dgcr8, xpo1, and xpo5 as well as most ago genes have been retained after the acipenserid-specific whole genome duplication, while ago1 and ago3 genes have lost one paralog. While most diploid vertebrates possess only a single copy of dicer1, we strikingly found four paralogs of this gene in the sterlet genome, derived from a tandem segmental duplication that occurred prior to the last whole genome duplication. ago1,3,4 and exportins1,5 look to be prone to additional segment duplications producing up to four-five paralog copies in ray-finned fishes. We demonstrate for the first time exon microsatellite amplification in the acipenserid drosha2 gene, resulting in a highly variable protein product, which may indicate sub- or neofunctionalization. Paralogous copies of most microRNA metabolism genes exhibit different expression profiles in various tissues and remain functional despite the rediploidization process. Subfunctionalization of microRNA processing gene paralogs may be beneficial for different pathways of microRNA metabolism. Genetic variability of microRNA processing genes may represent a substrate for natural selection, and, by increasing genetic plasticity, could facilitate adaptations to changing environments. 相似文献
3.
Combustion, Explosion, and Shock Waves - A phase-plane method is proposed to model flow fields bounded by constant-velocity detonation waves propagating in TNT charges. Similarity transformations... 相似文献
4.
5.
Heiner Fangerau 《Ethik in der Medizin》2011,18(3):87-88
6.
Dipl. iur. Dipl. krim. Andrea Kemper Dr. med. Michael Kölch Prof. Heiner Fangerau Prof. Dr. med. Jörg M. Fegert 《Ethik in der Medizin》2010,22(1):33-47
Definition of the problem Medical confidentiality, which is one of the main pillars of medical ethics, has taken a decisive role in the latest discussions about the improvement of child protection laws. Inexplicit and plurivalent arrangements and procedures of how to legally handle child abuse cases when persons with legal custody refuse to take further support are seen as an obstacle for effective child protection. Arguments Non-specific legal regulations lead to task uncertainty and hesitation on the side of health care professionals, which can result in the delay or even prevention of necessary actions. Therefore, new laws on the federal level have been passed in order to establish certainty with regard to professional secrecy and in order to foster networking among the actors in the field of child protection. Conclusion The paper analyses and discusses these legal attempts at strengthening child protection. Although some of the federal laws have the potential of clarification, the great number of laws and their explication restricts their impact. 相似文献
7.
Nadine Müller Sabine Ellinger Birgit Alteheld Gudrun Ulrich‐Merzenich Heiner K. Berthold Hans Vetter Peter Stehle 《Molecular nutrition & food research》2010,54(11):1636-1645
White tea (WT) is rich in flavan‐3‐ols as green tea (GT) and might provide health protective effects due to the strong antioxidant properties of flavan‐3‐ols. Since intervention studies with WT are lacking, we evaluated the effects of WT consumption on antioxidant status, antioxidant capacity and biomarkers of oxidative stress compared to water and GT. After an overnight fast, 70 healthy non‐smokers were randomized to consume 600 mL of WT, GT or water (control). Plasma (epi‐)catechin and epi(gallo)catechingallate, antioxidant capacity (Folin assay, trolox equivalent antioxidant capacity test), 8‐iso‐prostaglandin F2α, ascorbic acid and uric acid were determined before and several times within 8 h after consumption. DNA strand breaks were measured in vivo and ex vivo (H2O2 stimulation) in leukocytes. Plasma flavan‐3‐ols significantly increased after WT and GT ingestion. Trolox equivalent antioxidant capacity was lower after 5 h in controls versus WT (p=0.031) and GT (p=0.005). Folin‐Ciocalteu reducing capacity, ascorbic and uric acid as well as markers of oxidative stress (8‐iso‐prostaglandin‐F2α, DNA strand breaks) were not affected by the beverages. A short‐term increase of catechins does not change plasma antioxidant capacity in healthy subjects. Conclusions with respect to health protective effects of WT and GT on the basis of these biomarkers can, thus, not be drawn. 相似文献
8.
Removal of Stroop interference was obtained after exposure to words eliciting positive affect. This effect was predicted by personality systems interactions (PSI) theory, which assumes that (a) an abstract (high-level) representation of a difficult intention is generated in intention memory under certain conditions (e.g., when an action plan contains more than 1 step); (b) positive affect releases the inhibition of the pathway between intention memory and its output system. Interference removal is interpreted in terms of volitional facilitation. This effect posits a challenge to current cognitive models of the Stroop effect. Compared with alternative explanations, PSI theory can explain the data in a broader context: Implications of volitional facilitation and volitional inhibition for the study of prospective memory, alienation, procrastination, and rumination in depression are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
9.
10.
In meiosis, DNA break formation and repair are essential for the formation of crossovers between homologous chromosomes. Without crossover formation, faithful meiotic chromosome segregation and sexual reproduction cannot occur. Crossover formation is initiated by the programmed, meiosis-specific introduction of numerous DNA double-strand breaks, after which specific repair pathways promote recombination between homologous chromosomes. Despite its crucial nature, meiotic recombination is fraud with danger: When positioned or repaired inappropriately, DNA breaks can have catastrophic consequences on genome stability of the resulting gametes. As such, DNA break formation and repair needs to be carefully controlled. Within centromeres and surrounding regions (i.e., pericentromeres), meiotic crossover recombination is repressed in organisms ranging from yeast to humans, and a failure to do so is implicated in chromosome missegregation and developmental aneuploidy. (Peri)centromere sequence identity and organization diverge considerably across eukaryotes, yet suppression of meiotic DNA break formation and repair appear universal. Here, we discuss emerging work that has used budding and fission yeast systems to study the mechanisms underlying pericentromeric suppression of DNA break formation and repair. We particularly highlight a role for the kinetochore, a universally conserved, centromere-associated structure essential for chromosome segregation, in suppressing (peri)centromeric DNA break formation and repair. We discuss the current understanding of kinetochore-associated and chromosomal factors involved in this regulation and suggest future avenues of research. 相似文献