Quantification of Dendritic Spines Remodeling under Physiological Stimuli and in Pathological Conditions

Numerous brain diseases are associated with abnormalities in morphology and density of dendritic spines, small membranous protrusions whose structural geometry correlates with the strength of synaptic connections. Thus, the quantitative analysis of dendritic spines remodeling in microscopic images is one of the key elements towards understanding mechanisms of structural neuronal plasticity and bases of brain pathology. In the following article, we review experimental approaches designed to assess quantitative features of dendritic spines under physiological stimuli and in pathological conditions. We compare various methodological pipelines of biological models, sample preparation, data analysis, image acquisition, sample size, and statistical analysis. The methodology and results of relevant experiments are systematically summarized in a tabular form. In particular, we focus on quantitative data regarding the number of animals, cells, dendritic spines, types of studied parameters, size of observed changes, and their statistical significance.     

Fire safe,sustainable loose furnishing

The aim of this exploratory study has been to investigate the fire properties and environmental aspects of different upholstery material combinations, mainly for domestic applications. An analysis of the sustainability and circularity of selected textiles, along with lifecycle assessment, is used to qualitatively evaluate materials from an environmental perspective. The cone calorimeter was the primary tool used to screen 20 different material combinations from a fire performance perspective. It was found that textile covers of conventional fibres such as wool, cotton and polyester, can be improved by blending them with fire resistant speciality fibres. A new three‐dimensional web structure has been examined as an alternative padding material, showing preliminary promising fire properties with regard to ignition time, heat release rates and smoke production.     

Piperine Targets Different Drug Resistance Mechanisms in Human Ovarian Cancer Cell Lines Leading to Increased Sensitivity to Cytotoxic Drugs

Our goal was to examine the anticancer effects of piperine against the resistant human ovarian cancer cells and to explore the molecular mechanisms responsible for its anticancer effects. Our study used drug-sensitive ovarian cancer cell line W1 and its sublines resistant to paclitaxel (PAC) and topotecan (TOP). We analyzed the cytotoxic effect of piperine and cytostatic drugs using an MTT assay. The impact of piperine on protein expression was determined by immunofluorescence and Western blot. We also examined its effect on cell proliferation and migration. We noticed a different level of piperine resistance between cell lines. Piperine increases the cytotoxic effect of PAC and TOP in drug-resistant cells. We observed an increase in PTPRK expression correlated with decreased pTYR level after piperine treatment and downregulation of P-gp and BCRP expression. We also noted a decrease in COL3A1 and TGFBI expression in investigated cell lines and increased COL3A1 expression in media from W1PR2 cells. The expression of Ki67 protein and cell proliferation rate decreased after piperine treatment. Piperine markedly inhibited W1TR cell migration. Piperine can be considered a potential anticancer agent that can increase chemotherapy effectiveness in cancer patients.     

Stem Photosynthesis—A Key Element of Grass Pea (Lathyrus sativus L.) Acclimatisation to Salinity

Grass pea (Lathyrus sativus) is a leguminous plant of outstanding tolerance to abiotic stress. The aim of the presented study was to describe the mechanism of grass pea (Lathyrus sativus L.) photosynthetic apparatus acclimatisation strategies to salinity stress. The seedlings were cultivated in a hydroponic system in media containing various concentrations of NaCl (0, 50, and 100 mM), imitating none, moderate, and severe salinity, respectively, for three weeks. In order to characterise the function and structure of the photosynthetic apparatus, Chl a fluorescence, gas exchange measurements, proteome analysis, and Fourier-transform infrared spectroscopy (FT-IR) analysis were done inter alia. Significant differences in the response of the leaf and stem photosynthetic apparatus to severe salt stress were observed. Leaves became the place of harmful ion (Na⁺) accumulation, and the efficiency of their carboxylation decreased sharply. In turn, in stems, the reconstruction of the photosynthetic apparatus (antenna and photosystem complexes) activated alternative electron transport pathways, leading to effective ATP synthesis, which is required for the efficient translocation of Na⁺ to leaves. These changes enabled efficient stem carboxylation and made them the main source of assimilates. The observed changes indicate the high plasticity of grass pea photosynthetic apparatus, providing an effective mechanism of tolerance to salinity stress.     

The Numerical Comparison of Heat Transfer in a Coated and Fixed Bed of an Adsorption Chiller

Ecological adsorption technology is becoming a focus of attention by industry due to the utilization of low grade thermal energy sources for cooling production. It can be a promising part of sustainable development concept of the global economy. Therefore, research aiming at improving their performance i.e. Coefficient of Performance (COP) by optimizing the construction of sorption beds with a built in heat exchanger system is crucial. The heat transfer characteristics between the bed of porous media (sorbent) and surface of the heat exchanger system determine the heating power of an adsorption chiller. The HP increase can be obtained by heat transfer intensification due to the increase in the thermal conductivity of the sorbent layer in the vicinity of the heat exchanger’s surface. The novel modification of the sorbent layer structure is proposed in the paper in order to improve the heat transfer processes in the heat exchanger boundary layer. The analysis of desorption process conditions in the parametric model of a coated and fixed adsorption bed design is presented in the paper. The computational fluid dynamics (CFD) with conjugate heat transfer analysis is used to determine the crucial input parameters (temperature distribution in the sorbent bed) for further analytical calculations. The commercial code Ansys Fluent was used to perform numerical simulations. The developed computational model consisted of three subdomains representing heating water, heat exchanger material (copper) and sorbent (silica gel). The comparison of a novel coated design and a conventional fixed bed is discussed in the paper. The numerical analysis is based on experimental thermal conductivity measurements of the sorbent layer in different configurations, which were performed using Laser Flash Method.     

Strategies to improve the nutritional value of meat products: incorporation of bioactive compounds,reduction or elimination of harmful components and alternative technologies

Meat is an excellent source of protein with a good balance of amino acids. It is also rich in several minerals, vitamins and bioactive compounds, which play important roles in metabolism and are easily assimilated from meat compared with other foods. The nutritional value of meat is one of the factors that influence consumer acceptance. However, it can be significantly reduced during processing. Moreover, some operations, such as curing, smoking or fermentation, used for processing can lead to the formation of harmful components in meat, and hence, nutritionists associate high consumption of processed meat with an increased risk of several diseases such as cardiovascular disorders and cancers. This review deals with the distinctive aspects of the technological strategies that can be used to improve the nutritional value of meat products, including the incorporation of bioactive components, reduction or elimination of harmful components (e.g. nitrite/nitrate, fat) and alternative technologies (ultrasound).     

The Effect of Antibacterial Particle Incorporation on the Mechanical Properties,Biodegradability, and Biocompatibility of PLA and PHBV Composites

The composites based on polylactide (PLA) and poly (3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) (PHBV) with the addition of antibacterial particles: silver (Ag) and copper oxide (CuO) are characterized. Basic mechanical properties and biodegradation processes, as well as biocompatibility of materials with human cells are determined. The addition of Ag or CuO to the polymers do not significantly affect their mechanical properties, flammability, or biodegradation rate. However, several differences between the base materials are observed. PLA‐based composites have higher tensile and impact strength values, while PHBV‐based ones have a higher modulus of elasticity, as well as better mechanical properties at elevated temperatures. Concerning biocompatibility, each of the tested materials support the growth of fibroblasts over time, although large differences are observed in the initial cell attachment. The analysis of hydrolytic degradation effects on the structure of materials shows that PHBV degrades much faster than PLA. The results of this study confirm the good potential of the investigated biodegradable polymer composites with antibacterial particles for future biomedical applications.     

Virtual representation of the historic space riches

This Paper discusses the use of modern techniques to gather, convert and present the historic facts concerning the shape of the city—for an example of Warsaw. The aim of the project is to analyse chances of cooperative use of historic data base and contemporary software in intention to create virtual model of the old urban structure.

The deepest source of understanding the contemporary nature of the city is genuine consciousness of its history.