Photoregulation of Gene Expression with Ligand-Modified Caged siRNAs through Host/Guest Interaction

Small interfering RNA (siRNA) can effectively silence target genes through Argonate 2 (Ago2)-induced RNA interference (RNAi). It is very important to control siRNA activity in both spatial and temporal modes. Among different masking strategies, photocaging can be used to regulate gene expression through light irradiation with spatiotemporal and dose-dependent resolution. Many different caging strategies and caging groups have been reported for light-activated siRNA gene silencing. Herein, we describe a novel caging strategy that increases the blocking effect of RISC complex formation/process through host/guest (including ligand/receptor) interactions, thereby enhancing the inhibition of caged siRNA activity until light activation. This strategy can be used as a general approach to design caged siRNAs for the photomodulation of gene silencing of exogenous and endogenous genes.     

热解酸洗污泥用于氮氧化物还原的研究

金属表面处理过程排出的酸洗废水经处理产生的酸洗污泥,若是处理不合理就会引发极其严重的环境污染问题,经过热解处理后可以得到含有碳和重金属的固体产物.以热解酸洗污泥为还原剂,考察其脱硝性能以及反应温度、氧气浓度、反应时间对热解酸洗污泥脱硝效率的影响.结果 表明,该热解酸洗污泥有良好的脱硝性能,在反应温度为800℃,氧气浓度为1％的条件下,脱硝效率可达95.093％,可以作为脱除NOx的还原剂.热解酸洗污泥的脱硝效率随着温度的升高和氧气浓度的增加而降低,并且随着时间的推移,脱硝效率下降较快.     

Detecting Cysteine in Bioimaging with a Near-Infrared Probe Based on a Novel Fluorescence Quenching Mechanism

Near-infrared (NIR) fluorescent probes are very significant for detecting cysteine in biological systems. Herein, we report a highly selective and sensitive NIR turn-on fluorescent probe (BDP-NIR) based on BODIPY with large Stokes shift (105 nm) for detecting Cys. We clarified the sensing mechanism based on the different thiol-induced S_NAr substitution/rearrangement reaction of the probe with cysteine and homocysteine/glutathione, which leads to the corresponding amino- and thiol-BODIPY dyes with distinct photophysical properties. Moreover, a novel mechanism of fluorescence quenching was demonstrated by density functional theory calculation. The reason for the fluorescence quenching of the probe might be intersystem crossing (from singlet to triplet excited state). Moreover, BDP-NIR had a high linear dynamic range of 0–500 μM, which was promising for detecting cysteine quantificationally. Significantly, BDP-NIR was capable of sensing endogenous cysteine in living cells and in vivo.     

综合改进奇异谱分解和奇异值分解的齿轮故障特征提取方法

针对齿轮故障特征微弱，在强背景噪声下难以有效提取的问题，提出了一种改进奇异谱分解（ISSD）结合奇异值分解（SVD）的齿轮故障特征提取方法。针对奇异谱分解（SSD）算法中模态参数需凭经验选取的缺陷，基于散布熵优化算法对SSD算法进行了改进，在得到既定的一组奇异谱分量的基础上，根据峭度值最大准则筛选出了最佳奇异谱分量并进行了SVD处理，采用奇异值能量标准谱自适应地确定了信号重构阶数以还原信号和提高降噪效果。最后对信号进行包络解调以提取齿轮故障特征，将所提方法运用到仿真信号和齿轮实测信号中，并同传统包络谱、SSD包络谱以及经验模态分解结合SVD(EMD-SVD)方法进行了对比分析，结果表明，所提方法的降噪和特征提取效果更佳，能够更加有效地实现齿轮故障的判别。     

变频器在暖通空调行业中的应用

本文提出中央空调系统的一种变频调速改造方案，介绍了该系统的结构组成及运行原理，对系统各部分的作用进行了讨论。     

控制系统的非线性分析及设计

利用控制系统的计算机辅助设计(CACSD)的Matlab软件包，采用非线性分析设计法，对某雷达控制系统进行了分析与设计，实测性能良好，并在工程中得到应用。     

Learning Deep Patch representation for Probabilistic Graphical Model-Based Face Sketch Synthesis

International Journal of Computer Vision - Face sketch synthesis has a wide range of applications in both digital entertainment and law enforcement. State-of-the-art examplar-based methods...     

Reciprocal bi-directional generative adversarial network for cross-modal pedestrian re-identification

To improve the accuracy of cross-modal pedestrian re-identification,a reciprocal bi-directional generative adversarial network-based method is proposed.First,we build two generative adversarial networks to generate cross-modal heterogeneous images.Second,an associated loss is designed to pull close the distribution of features in latent space during the image translation between visible and infrared images so as to help the networks generate fake heterogeneous images that have high similarity with the real images.Finally,by concatenating the original and generated heterogeneous pedestrian images into the discriminative feature extraction network,images from different modalities can be unified into a common modality,thus suppressing the cross-modal gap.Representation learning and metric learning are utilized to achieve more discriminative pedestrian features.Comparative experiments are conducted on SYSU-MM01 and RegDB datasets to analyze the accuracy with different loss functions.Compared with other state-of-the-art cross-modal pedestrian re-identification methods,the proposed method achieves a higher accuracy and stronger robustness.     

基于Linux+ARM的多格式视频图像采集系统的设计

设计了基于S3C2440微处理器的视频图像采集系统,详细分析了该系统的启动过程。在一块裸板上搭建起整个视频图像采集系统。结合V4L2技术完成了支持YUV、MJPEG图像格式的USB摄像头和支持RGB图像格式的CMOS摄像头的多格式视频图像采集系统。测试结果表明,该系统可以在LCD显示屏上动态显示多格式摄像头所采集到的视频图像,且视频图像具有清晰度高、流畅性好、稳定性强等特点,具有进一步推广使用的广阔前景。