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排序方式: 共有160条查询结果,搜索用时 31 毫秒
1.
Jakub Drzmisek Daniel Stipl Denisa Petrackova Branislav Vecerek Ana Dienstbier 《International journal of molecular sciences》2021,22(2)
Bacterial pathogens sense specific cues associated with different host niches and integrate these signals to appropriately adjust the global gene expression. Bordetella pertussis is a Gram-negative, strictly human pathogen of the respiratory tract and the etiological agent of whooping cough (pertussis). Though B. pertussis does not cause invasive infections, previous results indicated that this reemerging pathogen responds to blood exposure. Here, omics RNA-seq and LC–MS/MS techniques were applied to determine the blood-responsive regulon of B. pertussis. These analyses revealed that direct contact with blood rewired global gene expression profiles in B. pertussis as the expression of almost 20% of all genes was significantly modulated. However, upon loss of contact with blood, the majority of blood-specific effects vanished, with the exception of several genes encoding the T3SS-secreted substrates. For the first time, the T3SS regulator BtrA was identified in culture supernatants of B. pertussis. Furthermore, proteomic analysis identified BP2259 protein as a novel secreted T3SS substrate, which is required for T3SS functionality. Collectively, presented data indicate that contact with blood represents an important cue for B. pertussis cells. 相似文献
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Nikolic Maria Vesna Labus Nebojsa J. Pavlovic Vera P. Markovic Smilja Lukovic Miloljub D. Tadic Nenad B. Vujancevic Jelena D. Vlahovic Branislav Pavlovic Vladimir B. 《Journal of Electroceramics》2020,45(4):135-147
Journal of Electroceramics - Nanocrystalline Zn2SnO4/SnO2 powder was obtained by a solid state reaction of ZnO and SnO2 nanopowders mixed in the molar ratio 1:1. The phase composition of the... 相似文献
5.
The transformation of fertility and family behaviour in post-socialist countries was quick and the changes were striking. An account of the changes is the subject of dispute and the mechanisms have still not been explained in a fully satisfying manner. Relatively little attention has been paid to the changes in spatial aspects concerning the broadening of changes in family behaviour. The aim of this paper is to answer the question as to whether changes spread stochastically in post-socialist countries or if there are obvious spatial patterns. The study tries to answer the questions whether there are any cores of changes which may be understood as innovations, if there is any spatial clustering and if the extent is hierarchically arranged. 相似文献
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Removal of oxygen from feedwater is of paramount importance for safe boiler operation. Insufficient extraction of oxygen in deaeration vessels will lead to limited efficiency of oxygen scavengers and unsafe plant operation. The presented sizing procedure for packed deaeration columns is based on the similarity theory between heat and mass transfer and existing procedures. The goal of the proposed technique is not to achieve optimization of column dimensions, but to ensure that there is no undersizing of the packing height. This approach is justified considering the relatively low capital costs of deaeration columns in boiler plants. The proposed equations are based on an experimental setup involving several hundred operating regimes which have been verified and confirmed to have a high degree of accuracy. 相似文献
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Rheological properties of hydroxyl‐terminated and end‐capped aliphatic hyperbranched polyesters 下载免费PDF全文
Branko Dunjic Srba Tasic Branislav Bozic Vesna Aleksandrovic‐Bondzic Marija S. Nikolic Jasna Djonlagic 《应用聚合物科学杂志》2015,132(7)
The rheological behavior of two series of aliphatic hyperbranched (HB) polyesters, based on 2,2‐bis(hydroxymethyl)propionic acid (bis‐MPA) and di‐trimethylol propane (Di‐TMP) as a tetrafunctional core, was studied. The effect of the size (pseudo‐generation number, from second to eight) and structure on the melt rheological properties was investigated for a series of hydroxyl‐terminated HB polyesters. In addition, the influence of the nature and degree of modification of the terminal OH groups in a series of fourth‐generation polyesters end‐capped with short and long alkyl chains and some aryl groups on the rheological properties was analyzed. The time–temperature superposition procedure was applied for the construction of master curves and for the analysis of the rheological properties of HB polyesters. The data obtained from WLF analysis of the HB polyesters showed that the values of the thermal coefficient of expansion of free volume αf and the fractional free volume at the glass transition temperature, fg, increase with increasing size of the HB polyesters. It was shown that the modified HB polyesters exhibited lower Tg and TG′=G″ temperatures, above which viscous became dominant over elastic behavior. From an analysis of the master curves of the modified HB polyesters, it was observed that with increasing degree of modification, both storage and loss modules and complex dynamic viscosity and apparent energy for viscoelastic relaxation decrease, because of reduced intermolecular hydrogen interactions. They do not exhibit a plateau of rubbery behavior, which confirms that no entanglements are present and that the molar masses are below the critical molar mass. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 41479. 相似文献
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Image segmentation or registration approaches that rely on a local search paradigm (e.g, Active Appearance Models, Active Contours) require an initialization that provides for considerable overlap or a coarse localization of the object to be segmented or localized. In this paper we propose an approach that does not need such an initialization, but localizes anatomical structures in a global manner by formulating the localization task as the solution of a Markov Random Field (MRF). 相似文献
9.
Confined states of a positronium (Ps) in the spherical and circular quantum dots (QDs) are theoretically investigated in two size quantization regimes: strong and weak. Two-band approximation of Kane’s dispersion law and parabolic dispersion law of charge carriers are considered. It is shown that electron-positron pair instability is a consequence of dimensionality reduction, not of the size quantization. The binding energies for the Ps in circular and spherical QDs are calculated. The Ps formation dependence on the QD radius is studied. 相似文献
10.
Branislav M. Ja?imovi?Srbislav B. Geni? Dimitrije R. Djordjevi?Nikola J. Budimir Marko S. Jari? 《Chemical Engineering Research and Design》2011,89(6):561-572
The paper deals with the tray-to-tray method for determining the required number of trays in columns with intensive entrainment of liquid when the operating line of the “dry working regime” is not straight and with a small concentration of diffusing component in gas. Presented calculations show that the number of transfer units for gas and liquid should be calculated using the AIChE method accompanied with longitudinal mixing according to Gilbert properly transformed for case of diffusion through stagnant film. The suggested method can be applied for TEG dehydration absorbers-columns for dehydration of natural gas using triethylene glycol with system factor SF = 0.5. 相似文献