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1.
Although today's engineering computer technology allows for precise analysis of the structural response of a building, it does not readily provide insight for economical design. Due to the complex nature of a modern tall building consisting of thousands of structural members, the traditional design method is generally highly iterative and time consuming. This paper describes an efficient computer-based technique for least-weight design of three-dimensional (3D) tall steel building frameworks under multiple lateral loading conditions. Stiffness constraints in terms of interstorey drifts are considered and optimum discrete member sizes are automatically selected from databases of commercial standard steel sections. The technique is remarkably efficient and the optimum design generally converges in a few cycles. The designs of two 3D lateral-load resisting building frameworks are presented as illustrations. The effectiveness and suitability of the technique for the design of large-scale tall steel building frameworks are discussed. 相似文献
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Product development is a collaborative activity more often than ever carried out by distributed design teams. It is critical to determine how sketches are used in such environments in order to improve the design process. Sketches produced by students participating in a collaborative design project of three European Universities are classified according to the intention of the designer when producing a sketch, the level of detail shown in the sketch and the phase when the sketch was produced. The adapted classification system used in this paper helps to analyse type of sketches with most variety of ideas. Furthermore, this paper reviews which type of sketches offers the most potential to be further developed. Results show that persuasive sketches offer the broadest range of ideas since they are produced as a combination of ideas from brainstorming sessions. Shared sketches help to achieve consensus in decision-making since the sketches are most likely to be produced by the entire group rather than individually. 相似文献
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Hilary K. Snow 《Material Religion》2017,13(1):107-109
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Hilary Schaffer Boudet Dilanka Chinthana Jayasundera Jennifer Davis 《Canadian Metallurgical Quarterly》2011,137(7):498-511
Despite the considerable scholarship focused on infrastructure investment in the developing world and the substantial sums of money spent each year on developing-country infrastructure, little attention has been given to understanding the drivers of conflict that shape the trajectory and cost structures of these massive investments. The manifestation of conflict among stakeholders in infrastructure projects ranges from the renegotiation of contract terms by project partners to popular protests among consumers of privatized services. The principal objective of this research is to identify combinations of country, project, and stakeholder factors that are associated with the emergence of legal and political conflict within natural gas and oil pipeline projects and water supply concessions and leases. The analysis includes data from 26 infrastructure projects spanning 31 countries and uses an analytical approach derived from Boolean algebra. Country-level characteristics, such as extent of democracy and rate of international NGO membership, are found to be important elements in the recipes for conflict among water supply projects but not for pipeline projects. Local impacts such as service price increases (water supply) and limited provision of oil and gas to the project host country (pipelines) are also important drivers of conflict for both subsectors. The involvement of one or more international financial institutions is also associated with the emergence of conflict in projects. Contrary to expectations, public consultation is associated with conflict in both subsectors. Overall, the study findings suggest that several factors associated with conflict in infrastructure projects can be minimized with careful project design. 相似文献
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Matthew L. Breeze Elisa Leyva‐Guerrero Grant R. Yeaman Yelena Dudin Ryan Akel Phil Brune Fred Claussen Cecil Dharmasri Jenny Golbach Rong Guo Carl Maxwell Laura Privalle Hilary Rogers Kai Liu Guomin Shan Michele Yarnall Denise Thiede Nancy Gillikin 《Journal of the American Oil Chemists' Society》2015,92(8):1085-1092
Soybean agglutinin (SBA) protein, also known as soybean lectin, is regarded as an anti‐nutrient due to its negative effect on the ability of monogastric animals to gain weight following consumption of raw soybean seed. Historically, SBA has been measured using a time‐consuming and cumbersome hemagglutination procedure. The objective of our research was to obtain a validated methodology that is precise and accurate in the measurement of SBA while allowing minimally equipped laboratories to effectively conduct the analysis, thus our focus was on evaluating an existing commercially available ELISA, an enzyme‐linked‐lectin‐assay (ELLA), and a hybrid ELISA/ELLA. A new ELLA technique that can detect and quantify lectins was chosen and modified specifically for the quantitation of SBA in soybean seed. The proposed ELLA methodology is similar to a standard sandwich ELISA, and uses polyacrylamide‐linked N‐acetylgalactosamine (Gal–NAc–PAA) for a capture phase and the biotinylated version (Gal–NAc–PAA–Biotin) for detection. Based upon the validation data, the ELLA method can precisely and accurately determine soybean lectin levels in soybean seed. The validated ELLA method was used to quantify SBA in nine commercial soybean varieties introduced between 1972 and 2008 and demonstrated that the natural variability of SBA is subject to the effects of genotype and environment. 相似文献
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LG Yu DG Fernig MR White DG Spiller P Appleton RC Evans I Grierson JA Smith H Davies OV Gerasimenko OH Petersen JD Milton JM Rhodes 《Canadian Metallurgical Quarterly》1999,274(8):4890-4899
The Galbeta1-3GalNAcalpha (TF antigen)-binding lectin (ABL) from the common edible mushroom (Agaricus bisporus) has a potent anti-proliferative effect without any apparent cytotoxicity. This unusual combination of properties prompted investigation of its mechanism of action. In contrast to soluble lectin, agarose-immobilized, and hence noninternalizable ABL had no effect on proliferation of HT29 colon cancer cells. Electron microscopy of HT29 cells incubated with fluorescein- and gold-conjugated ABL showed internalization of the lectin into endocytotic vesicles and multivesicular bodies. Confocal microscopy showed perinuclear accumulation of fluorescein isothiocyanate-conjugated lectin, which also inhibits HT29 cell proliferation, raising the possibility that the lectin might interfere with nuclear pore function. Transport of heat shock protein 70 into the nucleus in response to heat shock was blocked by preincubation of HT29 cells for 6 h with 40 micrograms/ml ABL. In digitonin-permeabilized cells, nuclear uptake of bovine albumin conjugated to a nuclear localization sequence (NLS)-containing peptide was also inhibited by a 15-min preincubation with 40-100 micrograms/ml ABL. In contrast, serum-stimulated nuclear translocation of mitogen-activated protein kinase, which is NLS-independent, was not affected by pretreatment of cells with the lectin. These results suggest that the anti-proliferative effect of ABL is likely to be a consequence of the lectin trafficking to the nuclear periphery, where it blocks NLS-dependent protein uptake into the nucleus. 相似文献