Identification,Phylogeny, and Comparative Expression of the Lipoxygenase Gene Family of the Aquatic Duckweed,Spirodela polyrhiza,during Growth and in Response to Methyl Jasmonate and Salt

Lipoxygenases (LOXs) (EC 1.13.11.12) catalyze the oxygenation of fatty acids and produce oxylipins, including the plant hormone jasmonic acid (JA) and its methyl ester, methyl jasmonate (MeJA). Little information is available about the LOX gene family in aquatic plants. We identified a novel LOX gene family comprising nine LOX genes in the aquatic plant Spirodela polyrhiza (greater duckweed). The reduced anatomy of S. polyrhiza did not lead to a reduction in LOX family genes. The 13-LOX subfamily, with seven genes, predominates, while the 9-LOX subfamily is reduced to two genes, an opposite trend from known LOX families of other plant species. As the 13-LOX subfamily is associated with the synthesis of JA/MeJA, its predominance in the Spirodela genome raises the possibility of a higher requirement for the hormone in the aquatic plant. JA-/MeJA-based feedback regulation during culture aging as well as the induction of LOX gene family members within 6 h of salt exposure are demonstrated.     

Efficient monitoring of hyperproperties using prefix trees

Hyperproperties, such as non-interference and observational determinism, relate multiple computation traces with each other and are thus not monitorable by tools that consider computations in isolation. We present the monitoring approach implemented in the latest version of \(\text {RVHyper}\), a runtime verification tool for hyperproperties. The input to the tool are specifications given in the temporal logic \(\text {HyperLTL}\), which extends linear-time temporal logic (LTL) with trace quantifiers and trace variables. \(\text {RVHyper}\) processes execution traces sequentially until a violation of the specification is detected. In this case, a counterexample, in the form of a set of traces, is returned. \(\text {RVHyper}\) employs a range of optimizations: a preprocessing analysis of the specification and a procedure that minimizes the traces that need to be stored during the monitoring process. In this article, we introduce a novel trace storage technique that arranges the traces in a tree-like structure to exploit partially equal traces. We evaluate \(\text {RVHyper}\) on existing benchmarks on secure information flow control, error correcting codes, and symmetry in hardware designs. As an example application outside of security, we show how \(\text {RVHyper}\) can be used to detect spurious dependencies in hardware designs.

     

Coupling osmotic dehydration with heat treatment for green papaya impregnated with blackberry juice solution

Slices (1.5 mm thick) of green papaya were impregnated through osmotic dehydration with a blackberry juice–sucrose solution to produce an intermediate moisture product. The effect of processing temperature (T) and sucrose-added molality (m_sucrose) on mass transfer during the operation was assessed, using a response surface methodology (RSM). The RSM was used to model water loss, sugar and anthocyanin gain during the process. Increasing sucrose molality resulted in increasing water loss and sugar gain, but decreasing anthocyanin gain. Water transfer therefore limits anthocyanin impregnation, but not sucrose incorporation. Afterwards, the impact of heat treatment at high temperatures was analysed, using numerical simulation. The conditions of the combined process, designed to achieve an anthocyanin-rich final product, are low sucrose-added molalities (sucrose molality < 1 mol kg^–1) and high processing temperatures (T > 50 ºC) for osmotic dehydration, coupled with high-temperature, short-time (HTST) heat treatments for product stabilisation.     

Novel measurement system for respiratory aerosols and droplets in indoor environments

The SARS-CoV-2 pandemic has created a great demand for a better understanding of the spread of viruses in indoor environments. A novel measurement system consisting of one portable aerosol-emitting mannequin (emitter) and a number of portable aerosol-absorbing mannequins (recipients) was developed that can measure the spread of aerosols and droplets that potentially contain infectious viruses. The emission of the virus from a human is simulated by using tracer particles solved in water. The recipients inhale the aerosols and droplets and quantify the level of solved tracer particles in their artificial lungs simultaneously over time. The mobile system can be arranged in a large variety of spreading scenarios in indoor environments and allows for quantification of the infection probability due to airborne virus spreading. This study shows the accuracy of the new measurement system and its ability to compare aerosol reduction measures such as regular ventilation or the use of a room air purifier.     

In situ injection of THF to trigger gas hydrate crystallization: Application to the evaluation of a kinetic hydrate promoter

This paper investigates an original method to efficiently trigger gas hydrate crystallization. This method consists of an in situ injection of a small amount of THF into an aqueous phase in contact with a gas-hydrate-former phase at pressure and temperature conditions inside the hydrate metastable zone. In the presence of a CO₂–CH₄ gas mixture, our results show that the THF injection induces immediate crystallization of a first hydrate containing THF. This triggers the formation of the CO₂–CH₄ binary hydrate as proven by the pressure and temperature reached at equilibrium. This experimental method, which “cancels out” the stochasticity of the hydrate crystallization, was used to evaluate the effect of the anionic surfactant SDS at different concentrations, on the formation kinetics of the CO₂–CH₄ hydrate. The results are discussed and compared with those published in a recent article (Ricaurte et al., 2013), where THF was not injected but present in the aqueous phase from the beginning and at much higher concentrations.     

Purchased energy and policy impacts in the US manufacturing sector

The US manufacturing sector, which consists of industries that produce durable and nondurable goods, accounts for about 30 % of all the final energy consumed in the country. In this study, manufacturing sector data coming primarily from the Annual Survey of Manufacturers are used to estimate the total impact of one mode of energy efficiency policy, market persuasion programs, on aggregate electricity consumption and energy expenditures. Using a panel model consisting of data for 184 industries, the findings indicate that the cumulative effects since 2002 of this policy mode is a reduction in 2010 electricity consumption of 5.4 %, of electricity expenditures of 2.4 %, and of all other fuel expenditures of 5.7 %. These estimates are derived after controlling for changes in output, other production inputs, and economic conditions. Particular attention in this study is given to the effects of a permanent shift in demand, and temporary business cycle shock, on model external validity.     

Estimating person-to-person variability in VOC emissions from personal care products used during showering

An increasing fraction of volatile organic compounds (VOC) emissions come from the domestic use of solvents, contained within myriad commonplace consumer products. Emission rates are often poorly characterized and depend significantly on individual behavior and specific product formulation and usage. Time-concentration profiles of volatile organic compounds (VOCs) arising from the use of a representative selection of personal care products (PCPs) during showering are generated, and person-to-person variability in emissions calculated. A panel of 18 participants used a standardized set of products, dosages, and application times during showering in a controlled indoor bathroom setting. Proton transfer mass spectrometry was used to measure the in-room VOC evolution of limonene (representing the sum of monoterpenes), benzyl alcohol, and ethanol. The release of VOCs had reproducible patterns between users, but noticeable variations in absolute peak concentrations, despite identical amounts of material being used. The amounts of VOC emitted to air for one showering activity were as follows: limonene (1.77 mg ± 42%), benzyl alcohol (1.07 mg ± 41%), and ethanol (0.33 mg ± 78%). Real-world emissions to air were between 1.3 and 11 times lower than bottom-up estimates based on dynamic headspace measurements of product emissions rates, likely a result of PCPs being washed away before VOC evaporation could occur.     

Impact sensitivity of nitroglycerin

Abstract

The impact sensitivity of liquid nitroglycerin, 50% initiation level, was observed to be near 1.8 kg?cm or 3.3 × 10³ j/m². The impact sensitivity of solid nitroglycerin, 50% initiation level, was observed by extrapolation to be near 200 kg?cm or 4.4 × 10⁵ j/m². The impact sensitivity of a mixture of solid and liquid NG was observed to be comparable to solid NG at the two stimulus levels tested. Thus, the presence of solid nitroglycerin reduces the impact sensitivity of liquid nitroglycerin.

The 50% initiation level for Class A cyclo-tetra-methylenetetranitramine was observed to be near 150 kg?cm or 3.3 × 10⁵ j/m² using the same instrument.     

Similarities and differences in four views of David.

In this article, each author gives a brief comment on similarities he or she sees throughout the case formulations captured in the articles by Watson (2010a), Silberschatz (2010), Goldfried (2010), and Caspar (2010). A systematic comparison is added. Overall, the impression is confirmed and maintained that if authors are ready to renounce the use of the jargon of their specific approach, many similarities can be found. There are nevertheless differences that would lead us to expect a different course of therapy and different effects beyond the main effect of recovery in the case of David, pursuing the conceptualization and interventions proposed by Watson (2010b). (PsycINFO Database Record (c) 2010 APA, all rights reserved)