Comparative study of the phenolics,antioxidant and metagenomic composition of novel soy whey-based beverages produced using three different water kefir microbiota

Water kefir microbiota was used to develop novel soy whey-based beverages that have antioxidant activity. In the present study, comparative phenolics, antioxidant and metagenomic composition of the soy whey beverages fermented using three different water kefir microbiota, named WKFS-A, WKFS-B and WKFS-C were investigated. WKFS-B beverage had the highest concentrations of isoflavone aglycones (208.73 ± 2.78 mg L⁻¹) and phenolic acids (132.33 ± 3.41 mg L⁻¹) compared with WKFS-A (193.88 ± 1.15 mg L⁻¹) and (91.73 ± 2.34 mg L⁻¹) and WKFS-C (160.63 ± 1.76 mg L⁻¹) and (97.13 ± 2.63 mg L⁻¹), respectively. The WKFS-B also showed higher DPPH and ABTS radical scavenging activity and ferric reducing antioxidant power compared with WKFS-A and WKFS-C beverages. Microbial species diversity index analysis showed that a higher concentration of isoflavone aglycones, phenolic acids and increased antioxidant activity in the WKFS-B beverage correlates with the higher relative abundance of Lactobacillus genus. This study thus revealed that Lactobacillus dominated water kefir microbiota produces soy whey beverages with high phenolic acids, isoflavone aglycones and antioxidant activity.     

The renewable energy market in Brazil: Current status and potential

Renewable energy offers a range of options with which to meet the growing demand for energy, particularly in the context of the pursuit (especially in developing countries) of economic development which takes into account social and environmental issues. Brazil has abundant natural sources of renewable energy, such as wind and solar power, hydraulic energy, small hydroelectric plants, ethanol and bio-diesel. These sources form part of the Brazilian strategy aimed at satisfying the demand for 6300 MW of fresh capacity per year arising out of projected economic growth of 5.1% per year over the next 10 years. Renewable energy sources currently provide 47.2% of the internal supply of primary energy in Brazil. Brazil has been pursuing a strategy of maintaining its renewable energy matrix and developing and providing incentives for further low carbon initiatives.In this study we set out an overview of the renewable energy options available in Brazil, their current status, the main positive results obtained to date and future potential. We describe the market for renewable energy in Brazil and examine specific public policies aimed at overcoming barriers to this market, thereby promoting its consolidation and expansion.     

Anticoagulant, Antioxidant and Antitumor Activities of Heterofucans from the Seaweed Dictyopteris delicatula

In the present study, six families of sulfated polysaccharides were obtained from seaweed Dictyopteris delicatula by proteolytic digestion, followed by acetone fractionation and molecular sieving on Sephadex G-100. Chemical analyses demonstrated that all polysaccharides contain heterofucans composed mainly of fucose, xylose, glucose, galactose, uronic acid, and sulfate. The fucans F0.5v and F0.7v at 1.0 mg/mL showed high ferric chelating activity (～45%), whereas fucans F1.3v (0.5 mg/mL) showed considerable reducing power, about 53.2% of the activity of vitamin C. The fucan F1.5v presented the most prominent anticoagulant activity. The best antiproliferative activity was found with fucans F1.3v and F0.7v. However, F1.3v activity was much higher than F0.7v inhibiting almost 100% of HeLa cell proliferation. These fucans have been selected for further studies on structural characterization as well as in vivo experiments, which are already in progress.     

Evaluation of the impact of access to electricity: A comparative analysis of South Africa,China, India and Brazil

Most developing countries include rural electrification programs in their efforts to improve social conditions. There are, however, several obstacles to the evaluation of such programs and therefore of their social, economic, environmental and energy impacts on the target population, particularly on impoverished communities located in remote areas. Evaluation of the efficacy of public policies aimed at rural electrification in South Africa, China, India and Brazil enables such actions to be quantified and re-considered so as to bolster the sustainability of their planning and implementation and also so as to enable comprehension of the significance of access to electricity in relation to other aspects of the drive to improve living standards. The provision of electric energy amounts to more than access to a public service and should be considered an essential right, in a context of social equity and justice, which permits social integration and the access to other equally essential services.     

Palm kernel shell as a lightweight aggregate for concrete

These concrete mixes of widely differing water/cement ratios were made using palm kernel shell as course aggregate. The properties tested include the physical properties of the shell, the compressive, flexural and tensile splitting strengths of the concrete. These properties were compared with those of similar concrete specimens made with crushed granite as course aggregate. Results of the tests suggest that palm kernel shell cannot produce concrete with compressive strength above 30 MPa. However, for concrete grade 25 and below, the material was found to compare favourably with other conventional aggregate such as crushed granite.     

Optical properties of evaporated PbSnS3 thin films

PbSnS3 is a ternary sulfide semiconductor material which has been relatively little studied in the literature. Thin films of this material were deposited on glass substrates by a thermal evaporation process and their optical properties investigated. The optical parameters were determined from the analysis of measured transmission spectra, at normal incidence, from 400–1500 nm, at room temperature. The fundamental absorption edge is placed at 1.04±0.05 eV and is forbidden in nature. Other absorption edges are obtained at 1.44 and 1.55±0.05 eV, with a nearby direct one at 1.68±0.05 eV. The peaks at 1.44 and 1.55 eV have been independently confirmed by the spectral photocurrent response of the films. © 1998 Kluwer Academic Publishers     

Crystallographic, molecular modeling, and biophysical characterization of the valine beta 67 (E11)-->threonine variant of hemoglobin

The crystal structure of the mutant deoxyhemoglobin in which the beta-globin Val67(E11) has been replaced with threonine [Fronticelli et al. (1993) Biochemistry 32, 1235-1242] has been determined at 2.2 A resolution. Prior to the crystal structure determination, molecular modeling indicated that the Thr67(E11) side chain hydroxyl group in the distal beta-heme pocket forms a hydrogen bond with the backbone carbonyl of His63(E7) and is within hydrogen-bonding distance of the N delta of His63(E7). The mutant crystal structure indicates only small changes in conformation in the vicinity of the E11 mutation confirming the molecular modeling predictions. Comparison of the structures of the mutant beta-subunits and recombinant porcine myoglobin with the identical mutation [Cameron et al. (1993) Biochemistry 32, 13061-13070] indicates similar conformations of residues in the distal heme pocket, but there is no water molecule associated with either of the threonines of the beta-subunits. The introduction of threonine into the distal heme pocket, despite having only small perturbations in the local structure, has a marked affect on the interaction with ligands. In the oxy derivative there is a 2-fold decrease in O2 affinity [Fronticelli et al. (1993) Biochemistry 32, 1235-1242], and the rate of autoxidation is increased by 2 orders of magnitude. In the CO derivative the IR spectrum shows modifications with respect to that of normal human hemoglobin, suggesting the presence of multiple CO conformers. In the nitrosyl derivative an interaction with the O gamma atom of Thr67(E11) is probably responsible for the 10-fold increase in the rate of NO release from the beta-subunits. In the aquomet derivative there is a 6-fold decrease in the rate of hemin dissociation suggesting an interaction of the Fe-coordinated water with the O gamma of Thr67(E11).     

Electrical properties of vacuum evaporated PbSnS3 thin films

Electrical conduction of evaporated PbSnS₃ films of thickness ranging between 0.1 and 2.0 μm were studied by measuring the dc current in both parallel (planar) and transverse (cross plane) directions to the substrate surface. Conduction mechanisms relevant to various regions of the current-voltage characteristics are discussed. The obtained film conductivities were of the order 10⁻⁵ S cm⁻¹ at room temperature and increased exponentially with increasing temperature. No consistent modification of the conductivity values and nature were observed when the films are doped with CdCl₂, PbCl₂ and CuI impurities. While planar conductivity activation energies were constant with voltage and increased slightly with deposition temperature, the cross plane values were found to depend on both voltage and film deposition temperature.     

Comparison of systematic search and database methods for constructing segments of protein structure

Two principal methods of determining the conformation of shortpieces of polypeptide backbone in proteins have been developed:using a database of known structures and systematicallygeneratingall conformations. In this paper, we compare the effectivenessof these two techniques. The completeness of the database forsegments of different lengths is examined and it is found tocontain most conformations for segments seven residues long,but to deteriorate rapidly for longer regions. When the databasesegment is to be incorporated into the rest of a structure,at least seven residues are required to build four new residues,because of the need to positionthe segment relative to the restof the structure.It is found that such positioning using flankingresidues results in large errors in the inserted region. Weconclude that the database method is currently not effectivefor comparative modeling, even for short segments. The systematicsearchprocedure is found to generate almost all structures of shortsegments found in proteinsIn contrast to the database method,low root mean square error structures are obtained for a setof trial segments embedded in the rest of a protein structure.Thus, it should be considered the method of choice.     

Ordered mesoporous carbon films prepared from 1,5-dihydroxynaphthalene/triblock copolymer composites

Ordered mesoporous carbon (D-COU-1) films were synthesized by an organic-organic self assembly method using 1,5-dihydroxynaphthalene as carbon source. Channel-like pores with a well-ordered hexagonal structure were observed by TEM observations. The thermal stability of the D-COU-1 film was compared with the COU-1 film prepared using resorcinol (R-COU-1). The XRD results indicate that the structure of D-COU-1 film was less shrinkable under carbonization compared to that of the R-COU-1 film.