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1.
Corey R. Nelson Tyler Mrozowich Sean M. Park Simmone Dsouza Amy Henrickson Justin R. J. Vigar Hans-Joachim Wieden Raymond J. Owens Borries Demeler Trushar R. Patel 《International journal of molecular sciences》2021,22(1)
Rift Valley fever virus (RVFV) is a mosquito-transmitted virus from the Bunyaviridae family that causes high rates of mortality and morbidity in humans and ruminant animals. Previous studies indicated that DEAD-box helicase 17 (DDX17) restricts RVFV replication by recognizing two primary non-coding RNAs in the S-segment of the genome: the intergenic region (IGR) and 5′ non-coding region (NCR). However, we lack molecular insights into the direct binding of DDX17 with RVFV non-coding RNAs and information on the unwinding of both non-coding RNAs by DDX17. Therefore, we performed an extensive biophysical analysis of the DDX17 helicase domain (DDX17135–555) and RVFV non-coding RNAs, IGR and 5’ NCR. The homogeneity studies using analytical ultracentrifugation indicated that DDX17135–555, IGR, and 5’ NCR are pure. Next, we performed small-angle X-ray scattering (SAXS) experiments, which suggested that DDX17 and both RNAs are homogenous as well. SAXS analysis also demonstrated that DDX17 is globular to an extent, whereas the RNAs adopt an extended conformation in solution. Subsequently, microscale thermophoresis (MST) experiments were performed to investigate the direct binding of DDX17 to the non-coding RNAs. The MST experiments demonstrated that DDX17 binds with the IGR and 5’ NCR with a dissociation constant of 5.77 ± 0.15 µM and 9.85 ± 0.11 µM, respectively. As DDX17135–555 is an RNA helicase, we next determined if it could unwind IGR and NCR. We developed a helicase assay using MST and fluorescently-labeled oligos, which suggested DDX17135–555 can unwind both RNAs. Overall, our study provides direct evidence of DDX17135–555 interacting with and unwinding RVFV non-coding regions. 相似文献
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Kamenan Koffi A. Jagadeesh Anooja Kre N’guessan Raymond Assanvo Edja Florentin Soman Suraj Unni K. N. Narayanan 《Journal of Materials Science: Materials in Electronics》2021,32(11):14207-14216
Journal of Materials Science: Materials in Electronics - We successfully fabricated dye sensitized solar cells (DSSCs) employing a quasi-solid state electrolyte based on pristine insulator natural... 相似文献
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The traditional flexible job shop scheduling problem (FJSP) considers machine flexibility but not worker flexibility. Given the influence and potential of human factors in improving production efficiency and decreasing the cost in practical production systems, we propose a mathematical model of an extended FJSP with worker flexibility (FJSPW). A hybrid artificial bee colony algorithm (HABCA) is presented to solve the proposed FJSPW. For the HABCA, effective encoding, decoding, crossover and mutation operators are designed, and a new effective local search method is developed to improve the speed and exploitation ability of the algorithm. The Taguchi method of Design of Experiments is used to obtain the best combination of key parameters of the HABCA. Extensive computational experiments carried out to compare the HABCA with some well-performing algorithms from the literature confirm that the proposed HABCA is more effective than these algorithms, especially on large-scale FJSPW instances. 相似文献
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Fabien Paulot David Paynter Vaishali Naik Sergey Malyshev Raymond Menzel Larry W. Horowitz 《International Journal of Hydrogen Energy》2021,46(24):13446-13460
Hydrogen (H2) has been proposed as an alternative energy carrier to reduce the carbon footprint and associated radiative forcing of the current energy system. Here, we describe the representation of H2 in the GFDL-AM4.1 model including updated emission inventories and improved representation of H2 soil removal, the dominant sink of H2. The model best captures the overall distribution of surface H2, including regional contrasts between climate zones, when vd(H2) is modulated by soil moisture, temperature, and soil carbon content. We estimate that the soil removal of H2 increases with warming (2–4% per K), with large uncertainties stemming from different regional response of soil moisture and soil carbon. We estimate that H2 causes an indirect radiative forcing of 0.84 mW m?2/(Tg(H2)yr?1) or 0.13 mW m?2 ppbv?1, primarily due to increasing CH4 lifetime and stratospheric water vapor production. 相似文献
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Hao Wu Lu Zhang Jinfan Yang Ruonan Bo Hongxu Du Kai Lin Dalin Zhang Mythili Ramachandran Yingbin Shen Yangxi Xu Xiangdong Xue Zhao Ma Aaron Raymond Lindstrom Randy Carney Tzu‐Yin Lin Yuanpei Li 《Advanced functional materials》2020,30(15)
Real‐time tracking of the dynamics change of self‐assembled nanostructures in physiological environments is crucial to improving their delivery efficiency and therapeutic effects. However, such tracking is impeded by the complex biological microenvironment leading to inhomogeneous distribution. A rotatable fluorescent ratio strategy is introduced that integrates aggregation‐induced emission (AIE) and aggregation‐caused quenching (ACQ) into one nanostructured system, termed AIE and ACQ fluorescence ratio (AAR). Following this strategy, an advanced probe, PEG5k‐TPE4‐ICGD4 (PTI), is developed to track the dynamics change. The extremely sharp fluorescent changes (up to 4008‐fold) in AAR allowed for the clear distinguishing and localization of the intact state and diverse dissociated states. The spatiotemporal distribution and structural dynamics of the PTI micelles can be tracked, quantitatively analyzed in living cells and animal tissue by the real‐time ratio map, and be used to monitor other responsive nanoplatforms. With this method, the dynamics of nanoparticle in different organelles are able to be investigated and validated by transmission electron microscopy. This novel strategy is generally applicable to many self‐assembled nanostructures for understanding delivery mechanism in living systems, ultimately to enhance their performance in biomedical applications. 相似文献