Towards Efficient Nonenzymatic DNA Ligation: Comparing Key Parameters for Maximizing Ligation Rates and Yields with Carbodiimide Activation**

Chemical ligation is an important tool for the generation of synthetic DNA structures, which are used for a wide range of applications. Surprisingly, reported chemical ligation yields can range from 30 to 95 % for the same chemical activating agent and comparable DNA structures. We report a systematic study of DNA ligation by using a well-defined bimolecular test system and a water-soluble carbodiimide (EDC) as a phosphate-activating agent. Our results emphasize the interplay between template-substrate complex stability and the rates of the chemical steps of ligation, with 3′ phosphate substrates providing yields near 100 % after 24 hours for particularly favorable reaction conditions. Ligation rates are also shown to be sensitive to the identity of the base pairs flanking a nick site, with as much as threefold variation. Finally, the observation that DNA substrates are modified by EDC at rates that can be comparable with ligation rates emphasizes the importance of considering side reactions when designing protocols to maximize ligation yields.     

Clearance angle and evolution of depth of cut in actuated disc cutting

This paper investigates the effect of cutter clearance angle on variation of depth of cut and cutting process with an actuated disc cutting(ADC). ADC is a cyclic cutting method with two main characteristics:(i) a disk-shape cutter is used to attack the rock in an undercutting mechanism; and(ii) the cutter is dynamically actuated as it is moved across the rock. Hence, the cutting process of such system is periodic, each recurrence known as actuation cycle. The first ADC model, developed in 2016, represented an idealization of the technology with a flat disc cutter, where no clearance angle was considered. The evolution of the contact between the disc and the rock was, therefore, computed only on horizontal x-y plane, ignoring the effect of normal component of the force acting on cartridge. This article reports on a study that incorporates the cutter inclination angle in derivation of cutter/rock interface laws. It extends the proposed kinematic and geometry based model to take into account the variable depth of cut in estimating the forces associated with cutting in one actuation cycle. Experiments were conducted using Wobble to test the predictions of the improved model at various operating conditions. The model predictions are matched with the experimental results and effects of various factors are analysed.     

Variable wall thickness scroll geometry modeling with use of a control volume approach

In a scroll-type compressor, compression is achieved through relative contact between two spiral curves. Since the scroll invention by Leon Creux (1905), multiple methods have been developed for calculating scroll geometry. What can generally be considered the most classical method, is defining each scroll curve as the involute of a circle. Gravesen and Henriksen (2001) introduced a new method to calculate scroll geometry by deriving each scroll curve from the radius of curvature parameterized with involute angle. This allows a wide range of involute geometries to be considered not included in the classical method. In this paper, Gravesen's method is extended to the tip region to include all tip geometries involved in a two arc configuration resulting in a more comprehensive scroll geometry definition. Lastly, with parametric representation of all scroll geometry, the pocket volume can be easily solved using a derived control volume approach.     

Highly Efficient,Solution‐Processed,Single‐Layer,Electrophosphorescent Diodes and the Effect of Molecular Dipole Moment

A new family of highly soluble electrophosphorescent dopants based on a series of tris‐cyclometalated iridium(III) complexes (1–4) of 2‐(carbazol‐3‐yl)‐4/5‐R‐pyridine ligands with varying molecular dipole strengths have been synthesized. Highly efficient, solution‐processed, single‐layer, electrophosphorescent diodes utilizing these complexes have been prepared and characterized. The high triplet energy poly(9‐vinylcarbazole) PVK is used as a host polymer doped with 2‐(4‐biphenylyl)‐5‐(4‐tert‐butyl‐phenyl)‐1,3,4‐oxadiazole (PBD) for electron transport. Devices with a current efficiency of 40 cd A^?1 corresponding to an EQE of 12% can thus be achieved. The effect of the type and position of the substituent (electron‐withdrawing group (CF₃) and electron‐donating group (OMe)) on the molecular dipole moment of the complexes has been investigated. A correlation between the absorption strength of the singlet metal‐to‐ligand charge‐transfer (¹MLCT) transition and the luminance spectral red shift as a function of solvent polarity is observed. The strength of the transition dipole moments for complexes 1–4 has also been obtained from TD‐DFT computations, and is found to be consistent with the observed molecular dipole moments of these complexes. The relatively long lifetime of the excitons of the phosphorescence (microseconds) compared to the charge‐carrier scattering time (less than nanoseconds), allows the transition dipole moment to be considered as a “quasi permanent dipole”. Therefore, the carrier mobility is sufficiently affected by the long‐lived transition dipole moments of the phosphorescent molecules, which are randomly oriented in the medium. The dopant dipoles cause positional and energetic disorder because of the locally modified polarization energy. Furthermore, the electron‐withdrawing group CF₃ induces strong carrier dispersion that enhances the electron mobility. Therefore, the strong transition dipole moment in complexes 3 and 4 perturbs both electron and hole mobilities, yielding a reduction in exciton formation and an increase in the device dark current, thereby decreasing the device efficiency.     

Combustion Properties of Amino‐Substituted Guanidinium 4,4′,5,5′‐Tetranitro‐2,2′‐biimidazolate(N4BIM) Salts

This paper describes the combustion properties of the amino‐substituted guanidinium 4,4′,5,5′‐tetranitro‐2,2′‐biimidazolate (N4BIM) series, including the bis‐mono, di and triaminoguanidinium salts. These salts are of interest as propellant ingredient additives, and in particular, the bis‐triaminoguanidinium salt of N4BIM displays excellent burn rate and combustion behavior. Our combustion studies have shown that TAGN4‐BIM displays a fast burning rate and has the lowest pressure dependence exponent yet measured for a triaminoguanidinium salt.     

Building test cases and oracles to automate the testing of web database applications

Many organizations rely on web applications that use back-end databases to store important data. Testing such applications requires significant effort. Manual testing alone is often impractical, so testers also rely on automated testing techniques. However, current automated testing techniques may produce false positives (or false negatives) even in a perfectly working system because the outcome of a test case depends on the state of the database which changes over time as data is inserted and deleted. The Automatic Database Tester (AutoDBT) generates functional test cases that account for database updates. AutoDBT takes as input a model of the application and a set of testing criteria. The model consists of a state transition diagram that shows how users navigate pages, a data specification that captures how data flows, and an update specification that shows how the database is updated. AutoDBT generates guard queries to determine whether the database is in a state conducive to performing and evaluating tests. AutoDBT also generates partial oracles to help validate whether a back-end database is updated correctly during testing. This paper describes the design of AutoDBT, a prototype implementation, several experiments with the prototype, and four case studies.     

The Propensity of Wooden Crevices to Smoldering Ignition by Firebrands

Fire Technology - Fires occurring at the wildland-urban interface (WUI) have rapidly increased in frequency and severity over the past few decades. As a result of these extreme fires, multiple...     

Complexation of organic compounds in the presence of Al3+ during micellar flocculation

Complexes of Al and model pollutants phenol, benzoic acid, 2,4-D (2,4-dichlorophenoxyacetic acid) and 2,4-DB (2,4-dichlorophenoxybutiric acid) play a key role in the removal of organic pollutants by co-adsorption on micelles of anionic surfactants flocculated by means of Al(3+). This technique, known as adsorptive micellar flocculation (AMF), is related to removal of organic compounds by coagulation-precipitation with Al and Fe salts. Some of the stoichiometries found (6Al:1L and higher, with "L" the organic compound) indicate that complexes of a cationic nature form in the presence of high excesses of Al, possibly polyaluminium complexes related to the species [Al(13)O(4)(OH)(24)](7+). In the case of phenol it has been established by spectroscopic measures that in the range of [Al(2)(SO(4))(3)](tot) between 5 x 10(-5) and 3 x 10(-4)M and [phenol](tot) between 10(-3) and 6 x 10(-3)M complexes exist at pH as low as 2.4. The results show that AMF may be explained by the chemisorption of molecules of organic compounds able to incorporate into the polyaluminic complexes occurring within the Stern layer of flocculating micelles.     

Advance measurement of gusts by laser anemometry

A coherent laser radar (or Doppler lidar) operating at a wavelength of 1.55 μm has been mounted on the nacelle of a 2.3 MW wind turbine in order to measure the wind speed in front of the blades at ranges up to 200 m. The lidar measures the component of wind speed along its beam direction but, since it rotates with the nacelle to point into the wind, this component normally differs negligibly from the actual wind speed. As an example to demonstrate the lidar's capabilities, we present samples of wind data gathered over an 18 h period in March 2003, illustrating the development of gusts that appears to be associated with the onset of solar ground heating. Wind speeds over the full period lay in the range 4-10 m s⁻¹, and the gusts were evident as a near-discontinuity in wind speed typically of order 1-2 m s⁻¹, but reaching values larger than 3 m s⁻¹. The typical timescale between gusts was of order 1 min. Improvements to this experiment are proposed that will provide more detailed information on spatial and temporal structure of such gusts.