A Novel Fluorescence Probe 9-（4-（1,2-diamine）benzeneN 1-phenyl）acridine for Nitric Oxide Determination

A novel fluorescent probe 9-(4-(1,2-diamine)benzene-N1-phenyl)acridine(DABPA) was synthesized for the detection of nitric oxide(NO) and characterized by IR, 1H-NMR and EI-MS spectroscopy. Based on a photoelectron transfer mechanism, the fl uorescence intensities of DABPA were investigated with the different concentrations of NO. Under the optimal experimental conditions, the fl uorescence intensity of DABPA had a good linear relationship(R2=0.9977) with NO concentration in the range from 1×10-7 to 1.5×10-6 mol/L with a detection limit of 1×10-8 mol/L. The cytotoxicity induced by DABPA was evaluated by the MTT(3-(4,5-dimethylthiazol-2-yl)-2,5diphenyl tetrazolium bromide) assay for biological application. Furthermore, the probe DABPA had also been successfully applied to real-time image NO produced in PC12 cells in the presence of L-arginine.     

化学复合CdS纳米粒子光折变聚合物研究

光折变聚合物是一种新型的光折变材料,化学复合纳米CdS粒子作为光敏剂在激光辐照下产生载流子,克服了物理掺杂易产生相分离等不利因素.以PVK 基体上化学复合CdS纳米粒子为基础,制备了有机-无机纳米光折变聚合物材料.对PVK-CdS薄膜进行了激光辐照下的光电导性能研究并对光折变样品膜进行了二波耦合实验,获得了较大的耦合增益系数.     

有机-无机纳米复合光折变材料的研究与进展

在聚合物中掺杂无机半导体纳米粒子作为光敏剂制备的有机-无机纳米复合光折变材料是一种新型的光折变材料.详细介绍了有机-无机纳米复合光折变材料的制备方法、光折变性能及其存在的问题,展望了这类新型材料的潜在优势和发展前景.     

Photorefractivity in a bi-functional polymer nanocomposites sensitized by CdS nanoparticle

We report an organic/inorganic hybridized nanocomposite consisting of a bi-functional poly(N-vinyl)-3-[p-nitrophenylazo]carbazolyl serves as a polymeric charge-transporting and second-order nonliner optical matrix, and CdS nanoparticles as photosensitizers to manifest photorefractive (PR) effect. The unpoled PVNPAK film exhibits a second harmonic generation (SHG) coefficient of 4.7 pm/V due to the possibility of self-alignment of the azo chromophore. Significant enhancement of photoconductivity is noticed with the increase of CdS nanoparticles concentration. The photorefractive property of the polymer nanocomposites were determined by two-beam coupling (TBC) experiment. The TBC gain and diffraction efficiency of 11.89 cm-1 and 3.2% were obtained for PVNPAK/CdS at zero electrical field.     

Fluorescence detection for H<Subscript>2</Subscript>PO<Subscript>4</Subscript><Superscript>-</Superscript> based on carbon dots/Fe<Superscript>3+</Superscript> composite

A novel fluorescent probe for H₂PO₄ ^- was designed and fabricated based on the carbon dots/Fe³⁺ composite. The carbon dots were synthesized by an established one-pot hydrothermal method and characterized by transmission electron microscope, X-ray diffractometer, UV-Vis absorption spectrometer and fluorescence spectrophotometer. The carbon dots/Fe³⁺ composite was obtained by aqueous mixing of carbon dots and FeCl₃, and its fluorescence property was characterized by fluorescence spectrophotometer. The fluorescence of carbon dots was quenched by aqueous Fe³⁺ cations, resulting in the low fluorescence intensity of the carbon dots/Fe³⁺ composite. On the other hand, H₂PO₄ ^- reduced the concentration of Fe³⁺ by chemical reaction and enhanced the fluorescence of the carbon dots/Fe³⁺ composite. The Stern-Volmer equation was introduced to describe the relation between the relative fluorescence intensity of the carbon dots/Fe³⁺ composite and the concentration of H₂PO₄ ^-, and a fine linearity (R ²=0.997) was found in the range of H₂PO₄ ^- concentration of 0.4-12 mM.     

NO光纤传感器研究进展

一氧化氮(NO)是生物体内一种重要的信使分子和效应分子,在生理和病理过程中起着重要作用,实现对生物体内NO快速、准确的检测,具有重要的意义,将光纤传感技术应用到对NO的检测中是目前最有前景的方法之一。本文综述了国内外NO光纤传感器的研究进展,介绍了几种适用于光纤传感器的新型NO探针和检测方法,并对NO光纤传感技术目前存在的问题及发展趋势进行了讨论。     

缓冲体系和金属离子对漆酶催化活性的影响

探讨了3种不同的缓冲体系和6种常见的金属离子对漆酶催化活性的影响.结果表明:不同的缓冲体系对漆酶催化活性的影响较大.在pH为2.0的磷酸氢二钾一柠檬酸缓冲体系(0.1 mol/L)中漆酶的催化活性最大.金属离子对漆酶催化活性的影响与金属离子的种类和浓度有关,在浓度低于0.1 mmol/L时,Pb2+、Ag+、Hg2+对漆酶的催化活性起激活作用,高于0.1 mmol/L时起抑制作用.在浓度低于20 mmol/L时,Mg2+、Zn2+、Cu2+对漆酶的催化活性起激活作用,高于20 mmol/L时起抑制作用.     

新型光纤葡萄糖复合敏感膜的研究

以四乙氧基硅烷(TEOS)为原料,采用溶胶-凝胶法将荧光指示剂(Ru(bpy)3Cl2)和葡萄糖氧化酶(GOD)分散于溶胶中干燥成膜.以葡萄糖溶液作为检测对象,采用锁相放大技术对复合敏感膜进行检测,研究了敏感膜的敏感特性和影响因素.实验结果表明:葡萄糖溶液浓度与复合敏感膜的滞后相移有较好的线性关系,检测线性范围是100-700 mg/dL;响应时间为30 s左右;检测的最佳pH值在7.0附近;重复性实验结果表明复合敏感膜相对偏差为±6.67%,复合敏感膜可以在12周内保持良好活力.     

Photoconductivity of novel poly（N-vinyl）-3 - [p-nitrophenylazo] carbazole/ CdS-nanoparticle polymer composite

The photoconductive characteristic of the inorganic/organic hybridized polymer system is reported, in which a novel bi-functional photorefractive （PR） poly（N-vinyl）-3-[p-nitrophenylazo]carbazole （PVNPAK） serves as a polymeric charge-transporting and second-order nonliner optical matrix and quantum dots composed of surface passivated cadmium sulfide serve as a charge-generation sensitizer. The hybrid PVNPAK/CdS-nanoparticles polymer composites with different mass ratio of CdS to PVNPAK were prepared. The generation of photocurrent on illumination and photoconductive properties of the PVNPAK/CdS-nanoparticles polymer composites were studied. The results show that the addition of CdS nanoparticle as a photosensitizer can enhance the photoconductivity of the PVNPAK significantly because of the properties of the high quantum efficiency of photosensitization and high charge transport to conducting polymer.     

白腐菌分泌漆酶的培养条件研究

采用液体摇瓶培养方法,探讨了金属离子、pH值、培养温度、碳源、氮源、表面活性剂、诱导剂等各种因素时白腐茵(Pleurotusostreatus)分泌漆酶能力的影响,采用正交实验对主要的影响因素进行了优化.结果表明,Cu2 、Mn2 的加入能促进白庸茵分泌漆酶,Ag 、Fe2 严重抑制白腐茵分泌漆酶,最佳碳源为葡萄糖、最佳氮源为蛋白胨,表面活性剂Tween-80和诱导荆的加入能显著提高漆酶的分泌.优化的培养条件为:土豆汁20%,葡萄糖20 g·L-1,蛋白胨4 g·L-1,CuSO4 0.5 mmol·L-1,KH2PO4 3 g·L-1,MgSO4 1.5 g·L-1,ZnSO4 50 rag·L-1,MnSO4 50 mg·L-1,维生素B1 2.0 mg·L-1,Tween-80 0.5 g·L-1,ABTS或XYL 1 mmol·L-1,pH值为4,28℃下摇瓶培养9 d,此时,漆酶酶活可达21 300 U·L-1.