甜叶菊随机扩增多态性DNA技术优化及亲缘关系研究

以甜叶菊为试材,对影响其随机扩增多态性DNA标记反应体系的7个因子进行优化,同时对来自国内外的12个品种进行亲缘关系分析。结果表明,20μL的优化体系包括:双蒸水13.6μL,10×Buffer(含15mmol/LMgCl2)溶液3μL,2.5mmol/L的dNTPS1.2μL,10μmol/L的引物1μL,20ng的模板DNA1μL,1UTaq聚合酶;热循环程序为:94℃预变性4min,94℃变性30s,35℃退火30s,72℃延伸1min,40个循环,最后72℃延伸7min。聚类图结果表明,品种间的遗传距离变异范围为0.12～0.88,在遗传距离为0.37时,8个引物可将12个品种分为四大类,来自日本的2个品种与国内品种关系较远。     

关于高校实验室建设的一点思考

面向21世纪的高校实验室建设普遍存在这样或那样的问题,本文从健全实验室管理体制、深化实验教学改革、加强实验技术队伍建设、开放实验室等方面入手,强调在构建新体系基础上实现实验室建设的可持续发展。     

Effects of lanthanum on POD expression and DNA methylation of purple pepper under salt stress

Pepper seedlings were hydroponically cultivated in 0-20 mg/L extraneous lanthanum chloride （La3＋） for different numbers of days to investigate its regulating effects under salt stress. The results showed that, depending on the salt stress of 0.75 g/L, a lower concentration of La3＋ （1.0 rag/L） might result in the lowest death rate, increase POD activity and change the band type of the stem and root on the 8th day. Furthermore, a methylation analysis by the methylation-sensitive amplified polymorphism （MSAP） technique indicated that a lower concentration of La3＋ （1.0 mg/L） triggered the lowest methylation level of 26.1%, of which the hypermethylation events were the primary type of methylation. In addition, the sequencing of 14 differ- entially expressed polymorphic fragments and the subsequent blast search revealed that La3＋ could induce methylation events in salt-tolerance sequences.     

谷氨酰胺、氯化镧对石斛兰离体增殖及叶片POD, CAT同工酶表达的影响

以石斛兰单株为外植体,研究了谷氨酰胺、氯化镧对石斛兰离体增殖及叶片POD,CAT同工酶表达的影响.结果表明,随着处理浓度、处理时间、外源添加物的不同,石斛兰增殖指标、叶片POD同工酶表达类型及活性差异明显,而CAT同工酶仅表现活性的差异.其中,处理4与处理5表现出截然相反的结果.随着处理时间的延长,特别是60d时,处理4的组培苗全部死亡,而处理5依然保持了100%最高的外植体增殖率和4.36最大的平均增殖苗数,而POD不仅表现在活性很高,且在B区比处理4多3条酶带.说明,0.05mg.L-1的氯化镧处理40d,不仅有利于石斛兰组培苗的增殖,也能够保证组培苗的质量.