Butyrate and Propionate Restore the Cytokine and House Dust Mite Compromised Barrier Function of Human Bronchial Airway Epithelial Cells

Barrier dysfunction of airway epithelium contributes to the development of allergies, airway hyper-responsiveness and immunological respiratory diseases. Short-chain fatty acids (SCFA) enhance and restore the barrier function of the intestinal epithelium. This study investigated whether acetate, propionate and butyrate enhance the integrity of bronchial epithelial cells. Differentiating human bronchial epithelial cells (16HBE) grown on transwells were exposed to butyrate, propionate and acetate while trans-epithelial electrical resistance was monitored over time. Restorative effects of SCFA were investigated by subsequent incubation of cells with IL-4, IL-13 or house dust mite extract and SCFA. SCFA effects on IL-4-induced cytokine production and the expression of zonula occludens-1 (ZO-1) and Mitogen-activated protein kinases (MAPK) signalling pathways were investigated by ELISA and Western blot assays. Propionate and butyrate enhanced the barrier function of differentiating 16HBE cells and induced complete recovery of the barrier function after exposure to the above-mentioned stimuli. Butyrate decreased IL-4-induced IL-6 production. IL-4 decreased ZO-1 protein expression and induced phosphorylation of extracellular signal-regulated protein kinases 1/2 (ERK1/2) and c-Jun N-terminal kinases (JNK) in 16HBE cells, both of which could be restored by SCFA. SCFA showed prophylactic and restorative effects on airway epithelial barrier function, which might be induced by increased ZO-1 expression.     

Two placebo-controlled crossover studies in healthy subjects to evaluate gastric acid neutralization by an alginate–antacid formulation (Gaviscon Double Action)

Objective: To investigate the intragastric acid neutralization activity of a combined alginate-antacid formulation.

Significance: Published studies have investigated the reflux-suppressing alginate component of Gaviscon Double Action (Gaviscon DA; RB, UK) but intragastric acid neutralization activity of the antacid component has not been evaluated in vivo.

Methods: Intragastric pH monitoring, using a custom-made 10-electrode catheter, was evaluated in a two-part exploratory study in healthy subjects; Part I (n?=?6) tested suitability of the catheter using antacid tablets (Rennie; Bayer, Germany); Part II (n?=?12) evaluated gastric acid neutralization activity of Gaviscon DA liquid (20?ml) versus placebo in fasted subjects using a randomized, open-label, crossover design. The primary endpoint was the percentage of time that intragastric pH ≥4 was measured during 30?min post-treatment. A confirmatory study of identical design was subsequently conducted (n?=?20).

Results: Monitoring pH using the multielectrode catheter was a viable approach, directly detecting changes in intragastric pH following a single dose of antacid tablets. In the exploratory study, the percentage of time that pH ≥4 during 30?minutes post-treatment was 46.8% with Gaviscon DA liquid versus 4.7% with placebo (p?=?0.0004). These findings were supported by the confirmatory study, where pH ≥4 was recorded 50.8% of the time with Gaviscon DA versus 3.5% with placebo (p?=?0.0051). In this study, Gaviscon DA was safe and well tolerated.

Conclusions: These studies demonstrate the effective acid neutralizing capacity of Gaviscon DA versus placebo in healthy, fasted subjects. This adds to the evidence base for the combination of alginates and antacids.     

Taxonomy of digital community currency platforms*

ABSTRACT

Community currencies are used to pay for products or services within specific groups defined by geographical boundaries or specific common interests. Financial crises, social emergence in developing countries, and increased access to digital devices have stimulated a growing number of communities worldwide to develop digital currency projects. These projects use technologies ranging from traditional plastic cards to mobile phones and blockchain technologies. Following the design science research approach, this paper analyzes digital community currencies (DCCs) by developing a taxonomy based on platform architecture, governance, transactionality and virtuality. By investigating 22 DCC platforms around the world, 4 groups were distinguished: local, proprietary, commons and cyber. The identification of these four different groups of digital community currencies allows us to better discuss the potentials and limitations of each one of them. The presented taxonomy can be useful to researchers and practitioners both to explain and to design DCC platforms. Discussing each of the emerging categories from the proposed taxonomy helps us to provide insights into DCCs, offering a new theoretical frame for investigating the particular case of digital payment platforms.     

A fuzzy logic approach to reliable real-time recognition of facial emotions

Multimedia Tools and Applications - This paper represents our newly developed software for emotion recognition from facial expressions. Besides allowing emotion recognition from image files and...     

The influence of cap rock composition on hydrocarbon seep type in the Zagros oil fields,a study using Advanced Spaceborne Thermal Emission and Reflection Radiometer mineral map

The persistent natural hydrocarbon seepage in onshore basins challenges observation and exploration technologies, which are required to document and assess these valuable indications of the presence of oil and gas in the subsurface. This paper aims at demonstrating the relationship between the compositional variation of an evaporite cap rock and the types of seeps occurring at the surface. For this purpose, the multispectral Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) data was utilized for mapping mineral variations of a petroleum system in the Zagros oil fields. Relative absorption-band depth (RBD), band rationing and the boosted regression trees (BRT) were applied to enhance and classify the mineral composition of evaporite, sandstone, and marly limestone formations. The gas seeps were associated with the areas of gypsum-bearing evaporite cap rock while oil seeps were mostly associated with calcite and clay zones within the cap rock, which was more prone to fracturing during the tectonic activities of the basin. It is suggested that the application of remote sensing in the oil and gas industry could be widened by detection of sleep-induced alteration to assess the efficiency of cap rock and to evaluate the productivity of reservoirs at a regional scale.     

Corrosion Behaviour of Type 316L Stainless Steel in Hot Caustic Aqueous Environments

Metals and Materials International - The corrosion behaviour of type 316L stainless steel in aqueous 30–50 wt%. NaOH at temperatures up to 90 °C has been elucidated....     

‘Land for infrastructure’ in Ho Chi Minh City: land-based financing of transportation improvement

This paper explores how land-based financing mechanisms are currently used in Ho Chi Minh City as a public-private funding strategy. The Land-for-Infrastructure (LFI) mechanism appears as a solution to produce infrastructures. We found that the implementation of the LFI mechanism remains difficult, but eventually can lead to success. By ‘trial-error-transcend,’ the City managed to build two essential roads while the developers received attractive investment opportunities in urban development. This mechanism cannot, however, be seen as a panacea for the local authorities due to constraints to replicate it and potential undesired ‘side effects.’     

Color preferences for different topics in connection to personal characteristics

Studies on color preferences are dependent on the topic and the relationships with personal characteristics, particularly personality, but these are seldom studied in one population. Therefore a questionnaire was collected from 1095 Dutch people asking for color preferences about different topics and relating them to personal characteristics. Color preferences regarding different topics show different patterns and significant differences were found between gender, age, education and personality such as being technical, being emotional or being a team player. Also, different colors were mentioned when asked for colors that stimulate to be quiet, energetic, and able to focus or creative. Probably, due to unconsciousness of contexts, many people had no color preference, a result that in the literature seldom is mentioned. Blue was the overall favorite color; however, most males chose for blue (25%) while most females had no color preference (18%). Black was the overall favorite color for clothing, mainly chosen by females (40%), while males primarily chose blue (27%). For building interiors subjects preferred white. For moods, subjects preferred white for being quiet or being able to focus, red for being energetic and had no color preference for being creative. It is concluded that color preferences are dependent upon the topic, and personal characteristics. The findings are important for architects, interior designers, fashion designers and product designers to have a basic idea of preferred colors for different objects by different types of people. © 2013 Wiley Periodicals, Inc. Col Res Appl, 40, 62–71, 2015     

Increasing inequalities in what we do online: A longitudinal cross sectional analysis of Internet activities among the Dutch population (2010 to 2013) over gender,age, education,and income

We investigate types of Internet activities among a representative sample of the Dutch population from 2010 to 2013. We examined usage patterns of seven types of Internet activities (i.e., information, news, personal development, commercial transaction, social interaction, leisure, and gaming) and related these patterns with gender, age, education, and income. Activities related to news, personal development, commercial transaction, and social interaction increased in popularity. For most capital enhancing activities, men, younger people, higher educated people, and people with higher than average incomes were prominent. These observations, however, are subject to change. The Internet seems to provide increasingly more capital-enhancing opportunities for those with higher education and income, which would accordingly reinforce their already strong positions in society.