Consensus design of repeat proteins

Consensus design is a valuable protein-engineering method that is based on statistical information derived from sequence alignments of homologous proteins. Recently, consensus design was adapted to repeat proteins. We discuss the potential of this novel repeat-based approach for the design of consensus repeat proteins and repeat protein libraries and summarize recent results from such experiments.     

Morphological and molecular characterization of Giardia isolated from the straw-necked ibis (Threskiornis spinicollis) in Western Australia

Following the first report of avian Giardia infection in Australia, isolates of the parasite recovered from naturally infected straw-necked ibis (Theskiornis spinicollis) were characterized using median body morphology, scanning electron microscopy, multilocus enzyme electrophoresis, random amplified polymorphic DNA (RAPD), and small subunit ribosomal RNA (SSU-rRNA) analyses. Results were compared with Giardia from other birds and mammals, and the extent of genetic diversity between a range of ibis isolates collected in Western Australia was determined. The ibis isolates of Giardia were genetically relatively homogeneous, which is in contrast to the extensive genetic heterogeneity often displayed by mammalian Giardia isolates. Morphologically, Giardia from ibis were similar to Giardia ardeae although they differed genetically and by the fact that the ibis isolates could not be established in in vitro culture. Sequence data of the DNA coding for the SSU-rRNA found a 96% homology between the ibis isolates from Western Australia and G. ardeae, suggesting that they represent distinct strains of the same species. In contrast, the ibis isolates were genetically and morphologically very different than Giardia duodenalis and Giardia muris from mammals.     

Experimentelle Untersuchung des Abwälzverhaltens von Beveloidverzahnungen mit lokal angepassten Verzahnungsdaten entlang der Zahnbreite

Forschung im Ingenieurwesen - Zur Übertragung von Drehmoment und Drehzahl in beliebiger Achsanordnung eignen sich bei kleinem Achswinkel bis 30° Beveloidverzahnungen. Besonders...     

Challenge of BALB/c mice with respiratory syncytial virus does not enhance the Th2 pathway induced after immunization with a recombinant G fusion protein, BBG2NA, in aluminum hydroxide

The polypeptide of aa 130-230 of the G protein (G2Na) of respiratory syncytial virus (RSV) was fused to BB, the albumin-binding region of streptococcal G protein, producing BBG2Na, which induced protective immune responses in rodent models. Evaluation of the immune response in mice immunized with BBG2Na in the adjuvant alhydrogel revealed high amounts of interleukin (IL)-5 and some IL-4 in splenocytes restimulated in vitro. This is compatible with a Th2 response. The activation of the Th2 pathway in such mice was further supported by the detection of IL-5 and G2Na-specific IgE in vivo. Of interest, in contrast to immunization with formalin-inactivated RSV, immunization of mice with BBG2Na followed by intranasal RSV challenge did not lead to increased production of IL-5- or G2Na-specific IgE. However, IgG1- and IgG2a-specific antibodies were boosted. These results demonstrate that the Th2 pathway is not enhanced by RSV challenge in BBG2Na-immunized mice.     

Analytical design method for beveloid gears with a small shaft angle and offset

Forschung im Ingenieurwesen - In addition to more sophisticated flank modifications, there is a discernible trend toward special gearing that is reflected in the increased use of beveloid...     

A molecular scanner to automate proteomic research and to display proteome images

Identification and characterization of all proteins expressed by a genome in biological samples represent major challenges in proteomics. Today's commonly used high-throughput approaches combine two-dimensional electrophoresis (2-DE) with peptide mass fingerprinting (PMF) analysis. Although automation is often possible, a number of limitations still adversely affect the rate of protein identification and annotation in 2-DE databases: the sequential excision process of pieces of gel containing protein; the enzymatic digestion step; the interpretation of mass spectra (reliability of identifications); and the manual updating of 2-DE databases. We present a highly automated method that generates a fully annoated 2-DE map. Using a parallel process, all proteins of a 2-DE are first simultaneously digested proteolytically and electro-transferred onto a poly(vinylidene difluoride) membrane. The membrane is then directly scanned by MALDI-TOF MS. After automated protein identification from the obtained peptide mass fingerprints using PeptIdent software (http://www.expasy.ch/tools/peptident.html + ++), a fully annotated 2-D map is created on-line. It is a multidimensional representation of a proteome that contains interpreted PMF data in addition to protein identification results. This "MS-imaging" method represents a major step toward the development of a clinical molecular scanner.     

How to adapt applications for the Cloud environment

The migration of existing applications to the Cloud requires adapting them to a new computing paradigm. Existing works have focused on migrating the whole application stack by means of virtualization and deployment on the Cloud, delegating the required adaptation effort to the level of resource management. With the proliferation of Cloud services allowing for more flexibility and better control over the application migration, the migration of individual application layers, or even individual architectural components to the Cloud, becomes possible. Towards this goal, in this work we focus on the challenges and solutions for each layer when migrating different parts of the application to the Cloud. We categorize different migration types and identify the potential impact and adaptation needs for each of these types on the application layers based on an exhaustive survey of the State of the Art. We also investigate various cross-cutting concerns that need to be considered for the migration of the application, and position them with respect to the identified migration types. Finally, we present some of the open research issues in the field and position our future work targeting these research questions.     

Motility of bacteria in microfluidic structures

Traditionally bacteria are cultivated on the surface of agar, an environment that is experimentally convenient, but it does not resemble the diverse micro-geometries of their natural habitat, such as abiotic (e.g., soil) and biotic (e.g., plant and other organisms) surfaces. In this work, microstructured environments were manufactured from a biocompatible polymer, polydimethylsiloxane, to test the motility behaviour of bacteria moving into complex, channel-like, closed geometries with different shapes and dimensions. The motile bacteria were imaged by light microscopy and observed in replicate trials. It was found that bacteria were capable of swimming through even narrow microchannels and that their swimming paths, velocity and modes were influenced by a combined effect of channel width and its complexity. These results show that the structured microfluidic environment can be used as a simple means to observe and quantify the movement and navigation behaviour of bacteria through geometrically heterogeneous environments.