Glossopharyngeal nerve transection impairs unconditioned avoidance of diverse bitter stimuli in rats.

There is growing evidence of heterogeneity among responses to bitter stimuli at the peripheral, central and behavioral levels. For instance, the glossopharyngeal (GL) nerve and neurons receiving its projections are more responsive to bitter stimuli than the chorda tympani (CT) nerve, and this is particularly true for some bitter stimuli like PROP & cycloheximide that stimulate the GL to a far greater extent. Given this information, we hypothesized that cutting the GL would have a greater effect on behavioral avoidance of cycloheximide and PROP than quinine and denatonium, which also stimulate the CT, albeit to a lesser degree than salts and acids. Forty male SD rats were divided into four surgery groups: bilateral GL transection (GLX), chorda tympani transection (CTX), SHAM surgery, and combined transection (CTX + GLX). Postsurgical avoidance functions were generated for the four bitter stimuli using a brief-access test. GLX significantly compromised avoidance compared to both CTX and SHAM groups for all stimuli (p     

Optimum stacking sequence design of composite laminates for maximum buckling load capacity using parameter-less optimization algorithms

This paper presents a comparative study of the application of parameter-less meta-heuristic algorithms in optimum stacking sequence design of com of composite laminates for maximum buckling load capacity. Here, JAYA algorithm, along with Salp Swarm Algorithm, Colliding Bodies Optimization, Grey Wolf Optimizer, and Genetic Algorithm with standard setting and self-adaptive version are implemented to the problem of composite laminates with 64 graphite/epoxy plies with conventional ply angles, under several bi-axial cases and panel aspect ratios. Optimization objective is to maximize the buckling load of symmetric and balanced laminated plate. Statistical analysis are performed for six cases and the results are compared in terms of mean, standard deviation, the coefficient of variation, best and worst solutions, accompanied by the percentage of the independent runs that found the global optimum \(\left( {{R_{{\text{op}}}}} \right)\) and near global optimum \(\left( {{R_{{\text{no}}}}} \right)\). The Kruskal–Wallis nonparametric test is also utilized to make further confidence in the examinations. Numerical results show the high capability of the JAYA algorithm for maximizing the buckling capacity of composite plates.

     

Sodium taste detectability in rats is dependent of anion size: The psychophysical characteristics of the transcellular sodium taste transduction pathway.

There are two known sodium transduction pathways in the rat gustatory system. The transcellular pathway is blocked by amiloride, and the paracellular pathway is limited by the anion gluconate. The contribution of each pathway to sodium detection was assessed. Sodium gluconate (NaGlu) and NaCl thresholds did not differ, implying that the paracellular pathway is not necessary for normal sodium detection. Adding 100 μM amiloride raised both NaCl and NaGlu thresholds but did not abolish all performance to NaGlu, indicating that some chemical cue was present at high concentrations. Rats were also exposed to one of three NaCl diets (0.12%. 1.0%, or 6.0% NaCl) through maternal and ad lib intake from Embryonic Day 1 through testing in adulthood. No differences across dietary groups were found for NaCl or NaGlu threshold with or without amiloride. Thus, this developmental dietary treatment does not appear to affect taste sensitivity to sodium subserved through either transduction pathway. Collectively, these data suggest that the transcellular transduction pathway is both necessary and sufficient for normal sodium detection. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Amiloride-insensitive units of the chorda tympani nerve are necessary for normal ammonium chloride detectability in the rat.

In spite of its common use as a standard stimulus in peripheral nerve recordings, relatively little is known about the psychophysics of NH?Cl taste. Rats' detection threshold for this salt was tested under a variety of conditions, including amiloride (100 μM) treatment and bilateral chorda tympani (CT) nerve transection. Detectability was measured with a 2-lever operant discrimination procedure used previously to measure detection thresholds for NaCl and KCl. Although NH?Cl and KCl appear to share a common taste quality and transduction mechanism, the logistic function and threshold for NH?Cl were found to be more similar to those of NaCl than to those of KCl. Like that of KCl, however, the detection threshold for NH4Cl increased significantly with CT transection (0.54 log?? units, p     

Robust design optimization of laminated plates under uncertain bounded buckling loads

Structural and Multidisciplinary Optimization - In this paper, a new systematic approach is suggested for better exploration of given uncertain buckling loads in the problem of optimal designs of...     

Anion Size Does Not Compromise Sodium Recognition by Rats After Acute Sodium Depletion.

Amiloride-insensitive sodium taste transduction is severely limited by large anions (i.e., gluconate). We found that in a brief-access taste test, sodium-depleted rats exhibited similar levels of increased licking to several sodium salts regardless of anion but did not increase licking to nonsodium salts compared with water. The enhanced licking of sodium salts was abolished in the presence of amiloride. These results suggest that the amiloride-sensitive taste transduction pathway is not only necessary but that it is also sufficient for sodium identification in rats. Sodium-depleted rats tested with amiloride initiated significantly more trials than nondepleted rats; hence, appetitive behavior was mildly potentiated by depletion, even in the absence of a sodium taste cue. Overall, these findings provide compelling support for the primacy of the amiloride-sensitive taste transduction mechanism and its associated neural pathway in the recognition of the sodium cation. (PsycINFO Database Record (c) 2010 APA, all rights reserved)