Identification of Host Cellular Protein Substrates of SARS-COV-2 Main Protease

The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease-19 (COVID-19) being associated with severe pneumonia. Like with other viruses, the interaction of SARS-CoV-2 with host cell proteins is necessary for successful replication, and cleavage of cellular targets by the viral protease also may contribute to the pathogenesis, but knowledge about the human proteins that are processed by the main protease (3CLpro) of SARS-CoV-2 is still limited. We tested the prediction potentials of two different in silico methods for the identification of SARS-CoV-2 3CLpro cleavage sites in human proteins. Short stretches of homologous host-pathogen protein sequences (SSHHPS) that are present in SARS-CoV-2 polyprotein and human proteins were identified using BLAST analysis, and the NetCorona 1.0 webserver was used to successfully predict cleavage sites, although this method was primarily developed for SARS-CoV. Human C-terminal-binding protein 1 (CTBP1) was found to be cleaved in vitro by SARS-CoV-2 3CLpro, the existence of the cleavage site was proved experimentally by using a His₆-MBP-mEYFP recombinant substrate containing the predicted target sequence. Our results highlight both potentials and limitations of the tested algorithms. The identification of candidate host substrates of 3CLpro may help better develop an understanding of the molecular mechanisms behind the replication and pathogenesis of SARS-CoV-2.     

东海盆地油气勘探焦点问题探讨

东海盆地自西向东可分为 3个带 ,即西部断陷带、中部隆起带和东部断坳带 ,其构造格局各具特色 ,并且均有自身的烃源岩。西部断陷带由多个以古新世为发育高峰期的半地堑组成 ,主要烃源岩为下古新统湖相泥岩 ,该带有利的勘探区是丽水、椒江凹陷 ,围绕下古新统主力烃源岩寻找油气是丽水、椒江凹陷油气勘探的焦点 ;中部隆起带是中生界残留盆地的叠置区 ,主要烃源岩为侏罗系湖相泥岩 ,福州凹陷是该带较有利的勘探区 ,烃源岩的规模、潜力以及圈闭保存条件是福州凹陷油气勘探的焦点 ;东部断坳带是厚度逾万米的新生代沉积断坳区 ,主要烃源岩为始新世煤系地层 ,该带有利的勘探区是西湖凹陷 ,烃源岩和盖层条件是西湖凹陷油气勘探的关键。目前 ,东海盆地比较现实和最具勘探前景的地区是西湖凹陷及丽水、椒江凹陷     

dU/dt引发的MOSFET误导通分析

分析了MOSFET误导通产生的原因,分别从原理和理论推导两个方面作了分析,主要考虑了开关管中寄生参数对开关特性的影响。通过求解误导通发生的条件,得到哪些参数会导致误触发,最后给出了仿真。文中还提出如何避免MOSFET误导通产生,以及改进方法,对减少实际应用中MOSFET破坏性损坏有一定意义。     

CMOS光接收机主放大器设计

利用CMOS工艺设计一种用于SDH STM 4速率级(622 Mb/s)光纤用户网的光接收机放大电路。此电路由输入/输出缓冲、主放大单元、偏置补偿电路4部分组成。通过直接耦合技术提高增益,降低功耗;利用有源电感负载提高系统带宽。采用商用SmartSpice电路仿真软件和CSMC HJ 0.6μm工艺参数对该电路进行仿真。结果表明,该电路在5 V工作电压下中频增益为81 dB,3 dB带宽为470 MHz。     

高校摄录编播设备数字化改造探析

为了用有限的资金建设一个高标准、现代化的适合高校现代教育技术需要的数字化摄录编播系统，结合实际，详细介绍一种适合高校重大事件记录、课件制作及教学片拍摄、可以满足高校多种需求的数字化摄录编播设备的合理化配置。     

万家寨引黄工程北干1号隧洞Ⅳ标155-274型TBM主轴承总成组装概要

文中对万家寨引黄工程北干线1号隧洞Ⅳ标使用的155—274型TBM主轴承总成各部件组装过程及其相应的技术要求进行了概要的描述，目的是进一步熟悉155—274型TBM主轴承总成的各个部件并加深对主轴承工作原理的理解。     

基于ARM微处理器的故障记录系统

给出了在普通数据采集基础上的故障记录系统的设计方案,利用ARM微处理器实现了模拟信号较完整的故障波形记录和开关信号的事件顺序记录(SOE)。对LPC2106ARM系列处理器、ADS7871模/数转换器以及数据通信接口等内容进行了讨论,阐述了系统的工作原理、软件处理方式、系统通信。该设计方案电路简单,特别适用于电力系统。     

浅谈冲压模具的维修要领及常见故障的排除

介绍冲压模具修理和检查各环节的主要工作内容及维修要领 ,列举了常见故障的排除方法 ,通过总结实际生产中的修模经验 ,对缩短修模周期 ,降低成本 ,延长模具寿命有一定的参考价值。。     

糯扎渡水电站机组主保护方案

结合糯扎渡水电站大型机组的发电机结构、发电机中性点引出方式及主接线对发电机主保护方案进行了分析研究，提出了发变组主保护方案，并初步确定了发电机中性点CT配置方案。同时也对主要的后备保护及异常运行保护进行了分析，并提出了设置方案。     

A time series bootstrap procedure for interpolation intervals

A sieve bootstrap procedure for constructing interpolation intervals for a general class of linear processes is proposed. This sieve bootstrap provides consistent estimators of the conditional distribution of the missing values, given the observed data. A Monte Carlo experiment is used to show the finite sample properties of the sieve bootstrap and finally, the performance of the proposed method is illustrated with a real data example.