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《分离科学与技术》2012,47(5):708-719
The forward extraction (FE) and backward extraction (BE) of humanized IgG4 Fab fragments using HDEHP-isooctane and -corn oil reverse micelles (RMs) with/without a non-ionic (Brij 30) and counterionic (TOMAC) surfactant, respectively, was evaluated, and both solvents were equally effective. Interphase precipitate formation during FE caused Fab fragment loss; however, acetone precipitation with an ethanol wash step enabled true percentage extractions to be determined. Brij 30 and TOMAC influenced extraction; TOMAC mainly increased BE at pH 8 with Brij 30 in FE. The highest extraction yields were without Brij 30 and TOMAC for isooctane and with TOMAC for corn oil. Water content analysis showed RMs formed in both solvents with/without Brij 30 and TOMAC, confirming extraction feasibility. 相似文献
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探讨了溶剂萃取工艺中萃取剂、有机溶剂的选择;水相pH值、萃取剂浓度、离子强度、相比的最佳工艺条件的确定.实验得到的萃取率可达99%以上,达到了治理环境,废物回收利用的目的. 相似文献
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研究了用三辛基甲基氯化铵(TOMAC)/正辛醇/异辛烷反胶团相转移法萃取细胞色素C时,水相pH值、离子强度、正辛醇含量、相体积比、搅拌时间等因素的影响。结果表明,正辛醇的量、水相pH值是萃取过程的关键因素,在低离子强度(CKCl=0.05mol/L)、高pH值(pH=13.66)、含正辛醇体积分数为0.85%的情况下,萃取率接近100%。 相似文献
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研究了三辛基甲基氯化铵(TOMAC)为载体的大块液膜体系处理含镍(Ⅱ)废水.应用正交实验考察了料液相中金属离子浓度、载体浓度、反萃剂浓度、料液相pH对镍(Ⅱ)迁移的影响.结果表明,镍(Ⅱ)迁移的最优方案为料液相中金属离子浓度为0.03mol/L,载体浓度为6%,反萃剂浓度为0.05mol/L,料液相pH为10.在最优条件下,迁移时间180min,迁移率可达53.92%.因素的影响主次为镍(Ⅱ)浓度〉料液相pH〉载体浓度〉反萃剂浓度. 相似文献
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