甲巯咪唑致中国人群胰岛素自身免疫综合征的临床特点分析

目的:探讨中国人群甲巯咪唑致胰岛素自身免疫综合征（IAS）的临床特点。方法:收集1985年1月1日至2019年6月30日国内外期刊公开发表的中国人群甲巯咪唑致IAS病例文献，进行回顾性分析。结果:共统计95例甲巯咪唑致IAS患者，男性发病年龄早于女性，性别比为1∶2.31；服用甲巯咪唑30 mg/d后在1月至3月内发生IAS最多，主要在夜间及凌晨以神经性低血糖为首发症状，血糖一般在2 mmol/L以下，胰岛素浓度常≥100 mU/ L，胰岛素自身抗体（IAA）阳性。胰腺影像学无明显异常；停用甲巯咪唑，给与对症及激素治疗后，症状逐渐缓解，激素治疗与非激素治疗低血糖消失时间无明显区别。结论:甲巯咪唑致IAS是一种临床上少见的自身免疫性疾病，服药期间如出现低血糖或高血糖现象，应及时就诊和处理，正确治疗后，一般预后良好。     

中文电子病历命名实体标注语料库构建

针对中文电子病历命名实体语料标注空白的现状,研究了中文电子病历命名实体标注语料库的构建。参考2010年美国国家集成生物与临床信息学研究中心(1282)给出的电子病历命名实体类型及修饰类型的定义,在专业医生的指导下制定了详尽的中文电子病历标注规范;通过对大量中文电子病历的分析,提出了一套完整的中文电子病历命名实体标注方案,而且采用预标注和正式标注的方法,建立了一定规模的中文电子病历命名实体标注语料库,其标注语料的一致性达到了92%以上。该工作对中文电子病历的命名实体识别及信息抽取研究提供了可靠的数据支持,对医疗知识挖掘也有重要意义。     

INDOLE-3-ACETIC ACID PRODUCTION BY GEL-ENTRAPPED BACILLUS THURINGIENSIS IN THE PRESENCE OF ROCK PHOSPHATE ORE

Bacillus thuringiensis in entrapped in k-carrageenan was capable of producing indole-3-acetic acid (IAA) in the presence of rock phosphate ore (RPO). After five repeated batch fermentation experiments, an average of 6.9 mg/L IAA was recorded in the presence of 1.5 g/L RPO and 4.7 mg/L IAA in the RPO-free control. The bacterium simultaneously solubilized phosphates with a maximum soluble P of 115 mg/L and 150 mg/L after the fourth batch cycle in 1.5 and 3.0 g/L RPO amendment, respectively. Addition of tryptophan to the media increased IAA production to 13.9 and 20.7 mg/L per batch on RPO-free and 1.5 g/L RPO-supplemented media with poor bacterial growth and RPO solubilization.     

海带生长素IAA分离纯化及高效液相色谱分析

对海带(Laminaria japonica Aresch.)中的生长素IAA的分离纯化条件进行优化,结果以超声法破碎细胞、采用体积分数95%乙醇浸提,乙醚萃取,硅胶脱色,然后通过Sephadex LH20柱层析为最佳条件,利用反向高效液相色谱(RP-HPLC)方法对IAA进行了定性和定量分析.实验表明:该方法操作简便、准确,重现性好,毒性较低,通过RP-HPLC结果可初步确定纯化产物中含有IAA,产量可达65～95μg/kg(鲜重).研究结果为进一步生物活性的研究和海带生长素及其他植物生长促进剂的开发和利用奠定了基础.     

Fe-chlorophyllin promotes the growth of wheat roots associated with nitric oxide generation

Effects of Fe-chlorophyllin on the growth of wheat root were investigated in this study. We found that Fe-chlorophyllin can promote root growth. The production of nitric oxide in wheat root was detected using DAF-2DA fluorescent emission. The intensity of fluorescent in the presence of 0.1 mg/L Fe-chlorophyllin was near to that observed with the positive control of sodium nitroprusside (SNP), the nitric oxide donor. IAA oxidase activity decreased with all treatments of Fe-chlorophyllin from 0.01 to 10 mg/L. At the relatively lower Fe-chlorophyllin concentration of 0.1 mg/L, the activity of IAA oxidase displayed a remarkable decrease, being 40.1% lower than the control. Meanwhile, Fe-chlorophyllin treatment could increase the activities of reactive oxygen scavenging enzymes, such as superoxide dismutase (SOD) and peroxidase (POD), as determined using non-denaturing polyacrylamide gel electrophoresis. These results indicate that Fe-chlorophyllin contributes to the growth of wheat root associated with nitric oxide generation.     

Growth Promotion of Yunnan Pine Early Seedlings in Response to Foliar Application of IAA and IBA

A field experiment was conducted using a 3 × 3 orthogonal regression design to explore the growth promotion of one-year-old Yunnan pine seedlings (Pinus yunnanensis Franch.) in response to foliar application of IAA (indole-3-acetic acid) at rates of 0, 200 and 400 mg·L(-1) and IBA (indole-3-butyric acid) at rates of 0, 200 and 400 mg·L(-1) in order to promote the growth during the seedlings' early stage. The experiment was conducted at the Lufeng Village Forest Farm of Yiliang County in Kunming, Yunnan, China. The results showed that IAA and IBA were effective in growth promotion of Yunnan pine seedlings. The response of both growth increment and biomass accumulation to the concentration of IAA and IBA can be modeled using a bivariate surface response, and each growth index had a peak value. Growth indexes increased with the increase of the dosage of photohormones before reaching a peak value, and then decreased. The different growth indexes had various responses to the concentrations and ratio of IAA and IBA. The foliar application of IAA in combination with IBA showed the largest improvement on the biomass of the needles, followed by stems and roots. The higher ratio of IAA promoted stem diameter growth, root system development and biomass accumulation in the needles, while a higher ratio of IBA contributed to height growth and biomass accumulation in the stem. Based on the auxin effect equations on the different growth indexes and surface response, the optimum concentrations and the (IAA:IBA) ratios can be obtained. The optimum concentrations of IAA and IBA were 167 and 186, 310 and 217, 193 and 159, 191 and 221, and 206 and 186 mg·L(-1), with corresponding ratios of 1:1.11, 1:0.70, 1:0.82, 1:1.15 and 1:0.90, respectively, at the maximum seedling height and collar diameter growth as well as biomass accumulation at the root, stem and needle. The above growth indexes were 22.00%, 79.80%, 48.65%, 82.20% and 107.00% higher than the control treatment.     

Crinumin,a chymotrypsin-like but glycosylated serine protease from Crinum asiaticum: Purification and physicochemical characterisation

Plant latex could be a potential source of novel proteases usable in the food and feed industries because of broad substrate specificity with high stability in extreme conditions. Crinumin, a glycosylated serine protease with chymotrypsin-like activity was purified from the latex of Crinumasiaticum using cation-exchange column chromatography. Crinumin shows activity over a wide range of pH (4.5–11.5 and optimum at 8.5), temperature (75 °C and optimum at 70 °C) and is also functional against chaotrophs, organic solvents, and detergents, even after prolonged exposure. The molecular mass (67.7 kDa), extinction coefficient (17.7), isoelectric point (6.9), and numbers of tryptophan (13), tyrosine (24) and cysteine (15 with 7 disulphide bridges) residues were estimated. K_m of the enzyme was 31.7 μM with casein and 5 × 10⁴ μM with N-succinyl-l-phenylalanine-p-nitroanilide. Easy availability of the aqueous latex, simple purification procedure, high yield (33%), stability and activity in adverse conditions makes it applicable for the pharmaceutical and food industries.     

Neriifolin S, a dimeric serine protease from Euphorbia neriifolia Linn.: Purification and biochemical characterisation

A dimeric serine protease Neriifolin S of molecular mass 94 kDa with milk clotting activity has been purified from the latex of Euphorbia neriifolia by anion exchange and size-exclusion chromatography. It hydrolyses peptidyl substrates l-Ala-pNA with highest affinity (K_m of 0.195 mM) and physiological efficiency (K_cat/K_m of 144.5 mM s). Enzyme belongs to the class of neutral proteases with pI value of 6.8, optimal proteolytic activity displayed at pH 9.5 and temperature 45 °C. Its proteolytic activity is strongly stimulated in the presence of Ca⁺² ions and exclusively inhibited by serine protease inhibitors. Enzyme is fairly stable toward chemical denaturants, pH and temperature. The apparent T_m, was found to be 65 °C. Thermal inactivation follow first order kinetics with activation energy (Ea), activation enthalpy (ΔH∗), free energy change (ΔG∗) and entropy (ΔS∗) of 27.54 kJ mol⁻¹, 24.89 kJ mol⁻¹, −82.34 kJ mol⁻¹ and 337.20 J mol⁻¹ K⁻¹.