In vitro evaluation of antifungal activity of soybean (Glycine max) seed coat proteins |
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| Authors: | PO Santos IS Santos VM Gomes OLT Machado KVS Fernandes J Xavier-Filho AEA Oliveira |
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| Affiliation: | aLaboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, 28013-602 Campos dos Goytacazes, RJ, Brazil;bLaboratório de Fisiologia e Bioquímica de Microrganismos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, 28013-602 Campos dos Goytacazes, RJ, Brazil |
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| Abstract: | Proteins in the soybean seed coat have previously been characterized; however, the function of these proteins is unknown. We show that a soybean seed coat protein fraction was able to inhibit the growth of Fusarium lateritium and Fusarium oxysporum phytopathogenic fungi. The antifungal fraction isolated by DEAE-Sepharose chromatography revealed the presence of peroxidase, vicilin and a 24 kDa protein homologous to acid phosphatases. Germination experiments revealed that both acid phosphatase and peroxidase were exuded during seed imbibition. We suggest that the set of seed coat antifungal proteins may help protect seeds from colonization by phytopathogenic fungi. |
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| Keywords: | Soybean Seed coat Phytopathogenic fungi |
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