利用HPLC-ELSD法测定烟用天然香精香料中10种水溶性糖

针对烟用天然香精香料样品，对YC/T 447—2012中糖的测定方法进行优化，建立了经水萃取后，直接利用HPLC-ELSD仪测定烟用天然香精香料中10种水溶性糖（包括鼠李糖、甜菊糖苷、果糖、山梨醇、甘露糖、半乳糖、葡萄糖、肌醇、蔗糖、麦芽糖）的分析方法。结果表明:①与0.02 mol/L NaOH溶液相比，利用水萃取后萃取溶液稳定性更好。②向流动相中添加一定量氨水后，各目标物保留时间更稳定。③ELSD气体流速为2.20 L/min，漂移管温度选择80 ℃时，各目标物峰面积稳定性更好。④10种水溶性糖的检出限在4.19~12.30 μg/mL之间，加标回收率在80.20%~106.00%之间，方法的日内、日间精密度均较好（日内RSD ≤ 4.05%、日间RSD ≤ 6.25%）。⑤采用所建立的分析方法对239种烟用天然香精香料中10种水溶性糖进行普查分析，其中检出率最高的5种糖类化合物为葡萄糖、果糖、蔗糖、麦芽糖、山梨醇。 

Quantification of 10 water-soluble sugars in natural tobacco flavors with HPLC-ELSD method

To quantify water-soluble sugars in natural tobacco flavors, the analytical method described in YC/T 447—2012 was optimized, and an HPLC-ELSD method with water extraction was developed to determine 10 water-soluble sugars in natural tobacco flavors, namely rhamnose, stevioside, fructose, sorbitol, mannose, galactose, glucose, inositol, sucrose, maltose. The results showed that: 1) The sample solution with water extraction presented better stability compared with the control method with 0.02 mol/L NaOH extraction. 2)The retention time of the analytes was more stable after adding aqueous ammonia into the mobile phase. 3)The stabilities of ELSD peak areas of the ananlytes were improved at a gas flow rate of 2.20 L/min and a drift tube temperature of 80 ℃. 4)The LODs of the 10 sugars were in the range of 4.19-12.30 μg/mL and the spiked recoveries ranged from 80.20% to 106.00% with good intra-day (RSD≤4.05%) and inter-day (RSD≤6.25%) precisions. 5) The contents of these sugars in 239 natural tobacco flavors were determined with the developed method, and the top five sugars were glucose, fructose, sucrose, maltose and sorbitol.